FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3042812104> ?p ?o ?g. }

• W3042812104 endingPage "5905" @default.
• W3042812104 startingPage "5905" @default.
• W3042812104 abstract "Mesenchymal stem cells derived from human bone marrow (hBM-MSCs) are utilized in tendon tissue-engineering protocols while extra-embryonic cord-derived, including from Wharton’s Jelly (hWJ-MSCs), are emerging as useful alternatives. To explore the tenogenic responsiveness of hBM-MSCs and hWJ-MSCs to human Growth Differentiation Factor 5 (hGDF-5) we supplemented each at doses of 1, 10, and 100 ng/mL of hGDF-5 and determined proliferation, morphology and time-dependent expression of tenogenic markers. We evaluated the expression of collagen types 1 (COL1A1) and 3 (COL3A1), Decorin (DCN), Scleraxis-A (SCX-A), Tenascin-C (TNC) and Tenomodulin (TNMD) noting the earliest and largest increase with 100 ng/mL. With 100 ng/mL, hBM-MSCs showed up-regulation of SCX-A (1.7-fold) at Day 1, TNC (1.3-fold) and TNMD (12-fold) at Day 8. hWJ-MSCs, at the same dose, showed up-regulation of COL1A1 (3-fold), DCN (2.7-fold), SCX-A (3.8-fold) and TNC (2.3-fold) after three days of culture. hWJ-MSCs also showed larger proliferation rate and marked aggregation into a tubular-shaped system at Day 7 (with 100 ng/mL of hGDF-5). Simultaneous to this, we explored the expression of pro-inflammatory (IL-6, TNF, IL-12A, IL-1β) and anti-inflammatory (IL-10, TGF-β1) cytokines across for both cell types. hBM-MSCs exhibited a better balance of pro-inflammatory and anti-inflammatory cytokines up-regulating IL-1β (11-fold) and IL-10 (10-fold) at Day 8; hWJ-MSCs, had a slight expression of IL-12A (1.5-fold), but a greater up-regulation of IL-10 (2.5-fold). Type 1 collagen and tenomodulin proteins, detected by immunofluorescence, confirming the greater protein expression when 100 ng/mL were supplemented. In the same conditions, both cell types showed specific alignment and shape modification with a length/width ratio increase, suggesting their response in activating tenogenic commitment events, and they both potential use in 3D in vitro tissue-engineering protocols." @default.
• W3042812104 created "2020-07-23" @default.
• W3042812104 creator A5024159880 @default.
• W3042812104 creator A5026122857 @default.
• W3042812104 creator A5030963147 @default.
• W3042812104 creator A5032805948 @default.
• W3042812104 creator A5073277207 @default.
• W3042812104 creator A5076775300 @default.
• W3042812104 creator A5087970528 @default.
• W3042812104 creator A5089940496 @default.
• W3042812104 date "2020-08-17" @default.
• W3042812104 modified "2023-10-05" @default.
• W3042812104 title "Dose-Response Tendon-Specific Markers Induction by Growth Differentiation Factor-5 in Human Bone Marrow and Umbilical Cord Mesenchymal Stem Cells" @default.
• W3042812104 cites W11651262 @default.
• W3042812104 cites W1491780525 @default.
• W3042812104 cites W1535485604 @default.
• W3042812104 cites W1557197409 @default.
• W3042812104 cites W1593255198 @default.
• W3042812104 cites W1963698651 @default.
• W3042812104 cites W1964368920 @default.
• W3042812104 cites W1964474774 @default.
• W3042812104 cites W1968323108 @default.
• W3042812104 cites W1970092955 @default.
• W3042812104 cites W1972930867 @default.
• W3042812104 cites W1974211526 @default.
• W3042812104 cites W1976906682 @default.
• W3042812104 cites W1996789930 @default.
• W3042812104 cites W2011074068 @default.
• W3042812104 cites W2012854380 @default.
• W3042812104 cites W2015642650 @default.
• W3042812104 cites W2016266407 @default.
• W3042812104 cites W2025112342 @default.
• W3042812104 cites W2025371271 @default.
• W3042812104 cites W2026513501 @default.
• W3042812104 cites W2046434476 @default.
• W3042812104 cites W2058400259 @default.
• W3042812104 cites W2058838426 @default.
• W3042812104 cites W2068022326 @default.
• W3042812104 cites W2071824292 @default.
• W3042812104 cites W2073007624 @default.
• W3042812104 cites W2080603184 @default.
• W3042812104 cites W2080605514 @default.
• W3042812104 cites W2083156208 @default.
• W3042812104 cites W2083375836 @default.
• W3042812104 cites W2084241194 @default.
• W3042812104 cites W2092054789 @default.
• W3042812104 cites W2095335313 @default.
• W3042812104 cites W2111174701 @default.
• W3042812104 cites W2115444861 @default.
• W3042812104 cites W2119610794 @default.
• W3042812104 cites W2128382826 @default.
• W3042812104 cites W2137188329 @default.
• W3042812104 cites W2138581811 @default.
• W3042812104 cites W2139779507 @default.
• W3042812104 cites W2142666026 @default.
• W3042812104 cites W2161006376 @default.
• W3042812104 cites W2168420558 @default.
• W3042812104 cites W2168838619 @default.
• W3042812104 cites W2186225470 @default.
• W3042812104 cites W2207538459 @default.
• W3042812104 cites W2269069106 @default.
• W3042812104 cites W2337861353 @default.
• W3042812104 cites W2409820086 @default.
• W3042812104 cites W2520593983 @default.
• W3042812104 cites W2581109444 @default.
• W3042812104 cites W2595526293 @default.
• W3042812104 cites W2614314413 @default.
• W3042812104 cites W2778968189 @default.
• W3042812104 cites W2807799059 @default.
• W3042812104 cites W2888926280 @default.
• W3042812104 cites W2914507740 @default.
• W3042812104 cites W2919207177 @default.
• W3042812104 cites W2940015059 @default.
• W3042812104 cites W2941661312 @default.
• W3042812104 cites W2952194887 @default.
• W3042812104 cites W3012071677 @default.
• W3042812104 cites W3013996114 @default.
• W3042812104 cites W3025949689 @default.
• W3042812104 cites W3026290253 @default.
• W3042812104 cites W3037256590 @default.
• W3042812104 cites W3043218165 @default.
• W3042812104 cites W3085647301 @default.
• W3042812104 cites W4242428523 @default.
• W3042812104 cites W4249556434 @default.
• W3042812104 doi "https://doi.org/10.3390/ijms21165905" @default.
• W3042812104 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7460605" @default.
• W3042812104 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32824547" @default.
• W3042812104 hasPublicationYear "2020" @default.
• W3042812104 type Work @default.
• W3042812104 sameAs 3042812104 @default.
• W3042812104 citedByCount "19" @default.
• W3042812104 countsByYear W30428121042020 @default.
• W3042812104 countsByYear W30428121042021 @default.
• W3042812104 countsByYear W30428121042022 @default.
• W3042812104 countsByYear W30428121042023 @default.
• W3042812104 crossrefType "journal-article" @default.
• W3042812104 hasAuthorship W3042812104A5024159880 @default.
• W3042812104 hasAuthorship W3042812104A5026122857 @default.

• next