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- W3042965504 abstract "Abstract Red blood cell (RBC) invasion by malaria merozoites involves formation of a parasitophorous vacuole into which the parasite moves. The vacuole membrane seals and pinches off behind the parasite through an unknown mechanism, enclosing the parasite within the RBC. During invasion, several parasite surface proteins are shed by a membrane-bound protease called SUB2. Here we show that genetic depletion of SUB2 abolishes shedding of a range of parasite proteins, identifying previously unrecognized SUB2 substrates. Interaction of SUB2-null merozoites with RBCs leads to either abortive invasion with rapid RBC lysis, or successful entry but developmental arrest. Selective failure to shed the most abundant SUB2 substrate, MSP1, reduces intracellular replication, whilst conditional ablation of the substrate AMA1 produces host RBC lysis. We conclude that SUB2 activity is critical for host RBC membrane sealing following parasite internalisation and for correct functioning of merozoite surface proteins. Key highlights Many malaria parasite surface proteins are shed by SUB2 during RBC invasion SUB2-null merozoites either induce rapid host RBC lysis, or invade then die Merozoite surface protein shedding is crucial for host RBC membrane sealing" @default.
- W3042965504 created "2020-07-23" @default.
- W3042965504 creator A5001119142 @default.
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- W3042965504 creator A5086755867 @default.
- W3042965504 date "2020-07-17" @default.
- W3042965504 modified "2023-10-14" @default.
- W3042965504 title "The malaria parasite sheddase SUB2 governs host red blood cell membrane sealing at invasion" @default.
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- W3042965504 cites W1895469649 @default.
- W3042965504 cites W1903847800 @default.
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- W3042965504 cites W1970883558 @default.
- W3042965504 cites W1974976084 @default.
- W3042965504 cites W1977364656 @default.
- W3042965504 cites W1980084408 @default.
- W3042965504 cites W1985652790 @default.
- W3042965504 cites W1991073542 @default.
- W3042965504 cites W1991465666 @default.
- W3042965504 cites W1994057928 @default.
- W3042965504 cites W2006741973 @default.
- W3042965504 cites W2010852041 @default.
- W3042965504 cites W2019697277 @default.
- W3042965504 cites W2025676593 @default.
- W3042965504 cites W2027825236 @default.
- W3042965504 cites W2028343031 @default.
- W3042965504 cites W2034720263 @default.
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- W3042965504 cites W2072900960 @default.
- W3042965504 cites W2073241690 @default.
- W3042965504 cites W2074089440 @default.
- W3042965504 cites W2074862264 @default.
- W3042965504 cites W2081626688 @default.
- W3042965504 cites W2082990282 @default.
- W3042965504 cites W2083385879 @default.
- W3042965504 cites W2084236168 @default.
- W3042965504 cites W2086376836 @default.
- W3042965504 cites W2087585115 @default.
- W3042965504 cites W2089035318 @default.
- W3042965504 cites W2090036244 @default.
- W3042965504 cites W2091304320 @default.
- W3042965504 cites W2098620195 @default.
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- W3042965504 cites W2112931002 @default.
- W3042965504 cites W2116741676 @default.
- W3042965504 cites W2120045745 @default.
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- W3042965504 cites W2140878929 @default.
- W3042965504 cites W2143096716 @default.
- W3042965504 cites W2143520814 @default.
- W3042965504 cites W2147295137 @default.
- W3042965504 cites W2155002191 @default.
- W3042965504 cites W2155966745 @default.
- W3042965504 cites W2162684905 @default.
- W3042965504 cites W2165626262 @default.
- W3042965504 cites W2168138163 @default.
- W3042965504 cites W2171236556 @default.
- W3042965504 cites W2208235309 @default.
- W3042965504 cites W2278831064 @default.
- W3042965504 cites W2328799122 @default.
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- W3042965504 cites W2810008525 @default.
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- W3042965504 doi "https://doi.org/10.1101/2020.07.15.205732" @default.
- W3042965504 hasPublicationYear "2020" @default.
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