Matches in SemOpenAlex for { <https://semopenalex.org/work/W3043018178> ?p ?o ?g. }
- W3043018178 endingPage "105707" @default.
- W3043018178 startingPage "105707" @default.
- W3043018178 abstract "14-3-3 protein isoforms regulate multiple processes in eukaryotes, including apoptosis and cell division. 14-3-3 proteins preferentially recognize phosphorylated unstructured motifs, justifying the protein-peptide binding approach to study 14-3-3/phosphotarget complexes. Tethering of human 14-3-3σ with partner phosphopeptides via a short linker has provided structural information equivalent to the use of synthetic phosphopeptides, simultaneously facilitating purification and crystallization. Nevertheless, the broader applicability to other 14-3-3 isoforms and phosphopeptides was unclear. Here, we designed a novel 14-3-3ζ chimera with a conserved phosphopeptide from BAD, whose complex with 14-3-3 is a gatekeeper of apoptosis regulation. The chimera could be bacterially expressed and purified without affinity tags. Co-expressed PKA efficiently phosphorylates BAD within the chimera and blocks its interaction with a known 14-3-3 phosphotarget, suggesting occupation of the 14-3-3 grooves by the tethered BAD phosphopeptide. Efficient crystallization of the engineered protein suggests suitability of the “chimeric” approach for studies of other relevant 14-3-3 complexes." @default.
- W3043018178 created "2020-07-23" @default.
- W3043018178 creator A5007825064 @default.
- W3043018178 creator A5026956570 @default.
- W3043018178 creator A5027061402 @default.
- W3043018178 creator A5029361837 @default.
- W3043018178 creator A5075390470 @default.
- W3043018178 date "2020-11-01" @default.
- W3043018178 modified "2023-10-18" @default.
- W3043018178 title "Design, expression, purification and crystallization of human 14-3-3ζ protein chimera with phosphopeptide from proapoptotic protein BAD" @default.
- W3043018178 cites W141855200 @default.
- W3043018178 cites W1547653016 @default.
- W3043018178 cites W1762454095 @default.
- W3043018178 cites W1963754786 @default.
- W3043018178 cites W1967294399 @default.
- W3043018178 cites W1970507353 @default.
- W3043018178 cites W1973312670 @default.
- W3043018178 cites W1974268113 @default.
- W3043018178 cites W1975689520 @default.
- W3043018178 cites W1975760429 @default.
- W3043018178 cites W1976062660 @default.
- W3043018178 cites W1978981437 @default.
- W3043018178 cites W1990032575 @default.
- W3043018178 cites W1990425248 @default.
- W3043018178 cites W2007486260 @default.
- W3043018178 cites W2010752097 @default.
- W3043018178 cites W2013452582 @default.
- W3043018178 cites W2015104840 @default.
- W3043018178 cites W2016269899 @default.
- W3043018178 cites W2017991274 @default.
- W3043018178 cites W2024923170 @default.
- W3043018178 cites W2027077560 @default.
- W3043018178 cites W2028541974 @default.
- W3043018178 cites W2030573603 @default.
- W3043018178 cites W2034359860 @default.
- W3043018178 cites W2037449911 @default.
- W3043018178 cites W2037481810 @default.
- W3043018178 cites W2044694582 @default.
- W3043018178 cites W2045022514 @default.
- W3043018178 cites W2048059179 @default.
- W3043018178 cites W2050054339 @default.
- W3043018178 cites W2051113354 @default.
- W3043018178 cites W2058285654 @default.
- W3043018178 cites W2065791607 @default.
- W3043018178 cites W2066324232 @default.
- W3043018178 cites W2070649124 @default.
- W3043018178 cites W2071075259 @default.
- W3043018178 cites W2073061210 @default.
- W3043018178 cites W2076928318 @default.
- W3043018178 cites W2078290510 @default.
- W3043018178 cites W2091816419 @default.
- W3043018178 cites W2094303284 @default.
- W3043018178 cites W2097767970 @default.
- W3043018178 cites W2099567270 @default.
- W3043018178 cites W2100059462 @default.
- W3043018178 cites W2100322860 @default.
- W3043018178 cites W2100837269 @default.
- W3043018178 cites W2102736458 @default.
- W3043018178 cites W2110844810 @default.
- W3043018178 cites W2122772601 @default.
- W3043018178 cites W2124443432 @default.
- W3043018178 cites W2128113729 @default.
- W3043018178 cites W2130408813 @default.
- W3043018178 cites W2131644398 @default.
- W3043018178 cites W2135116050 @default.
- W3043018178 cites W2138892400 @default.
- W3043018178 cites W2156016194 @default.
- W3043018178 cites W2158730778 @default.
- W3043018178 cites W2162404348 @default.
- W3043018178 cites W2164201106 @default.
- W3043018178 cites W2167630501 @default.
- W3043018178 cites W2206462941 @default.
- W3043018178 cites W2254034218 @default.
- W3043018178 cites W2577978946 @default.
- W3043018178 cites W2620975934 @default.
- W3043018178 cites W2763148184 @default.
- W3043018178 cites W2765854972 @default.
- W3043018178 cites W2768679340 @default.
- W3043018178 cites W2768995425 @default.
- W3043018178 cites W2806534677 @default.
- W3043018178 cites W2807814263 @default.
- W3043018178 cites W2896536135 @default.
- W3043018178 cites W2926185942 @default.
- W3043018178 cites W2949014628 @default.
- W3043018178 cites W2951997564 @default.
- W3043018178 cites W2980976402 @default.
- W3043018178 cites W3002983131 @default.
- W3043018178 cites W3015748930 @default.
- W3043018178 cites W3041244866 @default.
- W3043018178 doi "https://doi.org/10.1016/j.pep.2020.105707" @default.
- W3043018178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32682909" @default.
- W3043018178 hasPublicationYear "2020" @default.
- W3043018178 type Work @default.
- W3043018178 sameAs 3043018178 @default.
- W3043018178 citedByCount "6" @default.
- W3043018178 countsByYear W30430181782020 @default.
- W3043018178 countsByYear W30430181782021 @default.
- W3043018178 countsByYear W30430181782022 @default.