FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3043077928> ?p ?o ?g. }

• W3043077928 abstract "Purpose: Oral lichen planus (OLP) is a chronic condition characterised by T cell mediated destructions that is currently of unknown cause. OLP can be variably symptomatic with some patients experiencing no symptoms and others requiring extensive symptomatic management. Candida spp. can be found in association with OLP and due to this prophylactic treatment for Candida spp. is usually accounted for in the symptomatic management of OLP. This is despite current evidence not supporting concurrent use of antifungal therapy in the management of OLP with topical steroids.A potential hypothesis for the cause of OLP is an interaction of host genetic susceptibility combined with an environmental trigger that initiates disease in the susceptible host. Another equally likely hypothesis is that OLP is a true autoimmune condition with autoimmunity directed against a currently unknown epithelial autoantigen. The oral cavity represents a unique microenvironment that plays host to many commensal and opportunistic microorganisms. The oral microbiota, specifically Candida spp., could act as an aetiological trigger for the chronic T-cell mediated inflammation the defines OLP, specifically through activation of mucosal associated invariant (MAIT) cells. The role of Candida in the aetiopathogenesis and symptomatic management of OLP is currently unknown.Hypothesis and Aim: The overall hypothesis was that Candida may play an aetiological role in the OLP disease process exerting an effect on T cells and cytokine expression and that adjunctive treatment is required in the symptomatic management of OLP. The overall aim of this study was to determine whether Candida plays an aetiological role in OLP as well as determine if specific treatment of Candida is required in symptomatic patients with OLP.Materials and Methods: 14 control and 7 OLP test patients, 3 assigned to the placebo and 4 assigned to the antifungal treatment group, completed the clinical study. Assessments of clinical appearance, symptoms, Candida, salivary acetaldehyde and medication use were made at 0, 6 and 12 weeks for OLP patients with assessments of Candida and salivary acetaldehyde made at baseline only for controls. 20 random OLP formalin fixed paraffin embedded (FFPE) samples were stained using a fluorescent multiplex immunohistochemistry (mIHC) protocol for the markers cluster of differentiation (CD)3, CD8, DAPI, interleukin 18 receptor 1 (IL18R1), CD161, MR-1 and T cell receptor (TCR) V alpha 7.2. The slides were scanned with the Vectra Automated Multispectral Imaging System (PerkinElmer, USA) to generate multispectral images (MSI). The MSI were then analysed with tissue segmentation and single antibody algorithms for both HALO (Indica Labs, USA) and inForm 2.4.1 (PerkinElmer, USA) to validate a method for quantitative analysis. Following validation of HALO (Indica Labs, USA) for quantitative analysis the above process was repeated on 89 FFPE biopsy tissue samples from 73 patients with OLP (28 asymptomatic, 30 symptomatic and 16 samples with concurrent Candida (9 symptomatic and 7 asymptomatic), for comparison with 15 patient samples of fibroepithelial polyp (FEP). All samples were tested for presence of Candida with periodic acid-Schiff (PAS) staining. A BioPlex assay was performed to measure the cytokines interferon gamma, tumour necrosis factor alpha, interleukin (IL) 17A, IL-18, IL-12p40, IL-12p70, IL-22 and IL-23. Supernatant for this experiment was collected at 8, 12 and 24 hours following prior incubation of peripheral blood mononuclear cells (PBMC) in PBMC media supplemented with either 10% v/v effluent derived from C. albicans biofilms or 10% v/v artificial salivary media (ASM). In addition, some wells were supplemented with either CD28 and/or phorbol 12-myristate 13-acetate (PMA)/Ionomycin. Flow cytometry was performed using TCRV alpha 7.2, CD3, CD161, CD218a, CD4, CD8 and CD45 to define MAIT cells and T cell subsets. Prior to performing flow cytometry PBMC were incubated for 6 hours in PBMC media supplemented with either effluent derived from C. albicans biofilms or 10% v/v ASM with or without CD28.Results: Results of this study showed no significant differences existed between the control group and the OLP test group at baseline with respect levels of salivary acetaldehyde, and Candida colony forming units (CFU). Downward trends were noted in both groups with respect to clinical appearance and subjective analysis of symptoms from baseline to 12 weeks. Trends noted from assessment of CFU and salivary acetylaldehyde levels between the test groups should be viewed with caution due low levels of detection at baseline and the wide spread of data. Minor variability between the tissue segmentation algorithms with the trained algorithm for inForm 2.4.1 (PerkinElmer, USA) being the slightly less variable of the two. For quantitative cell analysis and identification of single antibody positive cells HALO (Indica Labs, USA) proved to be the least variable of the two trained algorithms. The presence of MAIT cell phenotypes were confirmed within the subepithelial infiltrate of OLP. Reduced MAIT cell phenotype expression was noted in the presence of Candida and/or symptoms in OLP with decreased expression of CD161 noted in the presence of symptoms whilst decreased expression of TCRV alpha 7.2 was noted in the presence of Candida. Presence of PMA/Ionomycin and Candida effluent were factors that increased the expression of interferon gamma, tumour necrosis factor alpha, IL-17A, IL-18, IL-22 and IL-23, cytokines that are associated with MAIT cell activation. Across all timepoints the presence of Candida effluent and CD28 resulted in upregulation of IL-18 and tumour necrosis factor alpha. MAIT cells were not significantly affected by the presence of either effluent or CD28 suggesting that neither Candida effluent nor CD28 alone or the combination of the two were shown to induce MAIT cell proliferation.Conclusion: Adjunctive treatment of symptomatic OLP with a topical antifungal did not significantly affect the presence of symptoms, erythema, CFU, Candida spp. or production of salivary acetaldehyde. HALO (Indica Labs, USA) was shown to be the more reliable program for mIHC quantitative cell analysis in FFPE OLP tissue. Analysis of mIHC in OLP FFPE tissue identified MAIT cells within the OLP inflammatory infiltrate with decreased expression of CD161 and TCRV alpha 7.2 noted in the presence of symptoms and Candida respectively. Finally, Candida effluent was unable to induce proliferation of MAIT cells in PBMC. However, cytokines associated MAIT cell activation and OLP, specifically interferon gamma, tumour necrosis factor alpha, IL-17A, IL-18, IL-22 and IL-23, were shown to be upregulated in the presence of Candida effluent derived from C. albicans biofilm." @default.
• W3043077928 created "2020-07-23" @default.
• W3043077928 creator A5063856730 @default.
• W3043077928 date "2019-01-01" @default.
• W3043077928 modified "2023-09-23" @default.
• W3043077928 title "The Role of Candida in Oral Lichen Planus (OLP)" @default.
• W3043077928 hasPublicationYear "2019" @default.
• W3043077928 type Work @default.
• W3043077928 sameAs 3043077928 @default.
• W3043077928 citedByCount "0" @default.
• W3043077928 crossrefType "dissertation" @default.
• W3043077928 hasAuthorship W3043077928A5063856730 @default.
• W3043077928 hasConcept C120149898 @default.
• W3043077928 hasConcept C16005928 @default.
• W3043077928 hasConcept C18903297 @default.
• W3043077928 hasConcept C2776201145 @default.
• W3043077928 hasConcept C71924100 @default.
• W3043077928 hasConcept C86803240 @default.
• W3043077928 hasConceptScore W3043077928C120149898 @default.
• W3043077928 hasConceptScore W3043077928C16005928 @default.
• W3043077928 hasConceptScore W3043077928C18903297 @default.
• W3043077928 hasConceptScore W3043077928C2776201145 @default.
• W3043077928 hasConceptScore W3043077928C71924100 @default.
• W3043077928 hasConceptScore W3043077928C86803240 @default.
• W3043077928 hasLocation W30430779281 @default.
• W3043077928 hasOpenAccess W3043077928 @default.
• W3043077928 hasPrimaryLocation W30430779281 @default.
• W3043077928 hasRelatedWork W2057921425 @default.
• W3043077928 hasRelatedWork W2106456109 @default.
• W3043077928 hasRelatedWork W2173122479 @default.
• W3043077928 hasRelatedWork W2188534493 @default.
• W3043077928 hasRelatedWork W2364547466 @default.
• W3043077928 hasRelatedWork W2375534637 @default.
• W3043077928 hasRelatedWork W2389180826 @default.
• W3043077928 hasRelatedWork W2392053734 @default.
• W3043077928 hasRelatedWork W2474489209 @default.
• W3043077928 hasRelatedWork W2516415303 @default.
• W3043077928 hasRelatedWork W2527722260 @default.
• W3043077928 hasRelatedWork W2797273526 @default.
• W3043077928 hasRelatedWork W2901500427 @default.
• W3043077928 hasRelatedWork W2966469007 @default.
• W3043077928 hasRelatedWork W2994866323 @default.
• W3043077928 hasRelatedWork W3005051972 @default.
• W3043077928 hasRelatedWork W3094262830 @default.
• W3043077928 hasRelatedWork W3129004991 @default.
• W3043077928 hasRelatedWork W3140956622 @default.
• W3043077928 hasRelatedWork W3143042789 @default.
• W3043077928 isParatext "false" @default.
• W3043077928 isRetracted "false" @default.
• W3043077928 magId "3043077928" @default.
• W3043077928 workType "dissertation" @default.