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- W3043092316 abstract "Single-cell RNA sequencing (scRNA-seq) allows for unbiased transcriptomic characterization at high throughput and has been rapidly adopted by scientists across disciplines. 1 Hedlund E. Deng Q. Single-cell RNA sequencing: technical advancements and biological applications. Mol Aspects Med. 2018; 59: 36-46 Crossref PubMed Scopus (169) Google Scholar Since it was first described in 2009, scRNA-seq has been leveraged to study cell heterogeneity, lineage specification, fate determination, and tissue remodeling, including both kidney development and fibrosis. 2 Wu H. Humphreys B.D. The promise of single-cell RNA sequencing for kidney disease investigation. Kidney Int. 2017; 92: 1334-1342 Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar ,3 Lindström N.O. De Sena Brandine G. Tran T. et al. Progressive recruitment of mesenchymal progenitors reveals a time-dependent process of cell fate acquisition in mouse and human nephrogenesis. Dev Cell. 2018; 45: 651-660.e4 Abstract Full Text Full Text PDF PubMed Scopus (108) Google Scholar Technological advances have vastly improved the numbers of cells analyzed and the sensitivity of gene detection while dramatically reducing costs. 1 Hedlund E. Deng Q. Single-cell RNA sequencing: technical advancements and biological applications. Mol Aspects Med. 2018; 59: 36-46 Crossref PubMed Scopus (169) Google Scholar For example, commonly used microfluidics devices are now able to process 80,000 cells (>3000 genes per cell) in a 1-day laboratory workflow. 2 Wu H. Humphreys B.D. The promise of single-cell RNA sequencing for kidney disease investigation. Kidney Int. 2017; 92: 1334-1342 Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar" @default.
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- W3043092316 date "2020-12-01" @default.
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- W3043092316 title "Surveying the human single-cell landscape" @default.
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- W3043092316 doi "https://doi.org/10.1016/j.kint.2020.06.027" @default.
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