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- W3043102034 endingPage "127568" @default.
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- W3043102034 abstract "Escherichia coli O157:H7 makes a major threat to human health. Aiming to detect Escherichia coli O157:H7 sensitively, hybridization chain reaction signal amplified immunoassay (immuno-HCR) based on contact quenching (CQ) and fluorescence resonance energy transfer (FRET) was developed. The background of the new designed HCR hairpins (CQ-FRET hairpins) was reduced by contact-quenching fluorescein (FAM) and breaking FRET from donor (FAM) to acceptor (Cy5). The F/F0 ratio of CQ-FRET hairpins (37.02) was obviously higher than that of two other common HCR fluorescent hairpins (CQ hairpins, 21.45; FRET hairpins, 4.61). The limit of detection of the assay was 3.5 × 101 CFU/mL and obviously lower than that of CQ hairpins based immuno-HCR (3.28 × 103 CFU/mL) and FRET hairpins based immuno-HCR (6.49 × 104 CFU/mL). The proposed low fluorescent background immuno-HCR with high sensitivity which was verified in contaminated milk samples could be potentially used in the detection of various pathogens." @default.
- W3043102034 created "2020-07-23" @default.
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- W3043102034 date "2021-01-01" @default.
- W3043102034 modified "2023-10-18" @default.
- W3043102034 title "Immuno-HCR based on contact quenching and fluorescence resonance energy transfer for sensitive and low background detection of Escherichia coli O157:H7" @default.
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- W3043102034 doi "https://doi.org/10.1016/j.foodchem.2020.127568" @default.
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