Matches in SemOpenAlex for { <https://semopenalex.org/work/W3043205850> ?p ?o ?g. }
- W3043205850 abstract "Abstract Aberrant DNA methylation in the region surrounding the transcription start site is a hallmark of gene silencing in cancer. Currently approved demethylating agents lack specificity and exhibit high toxicity. Herein we show, using the p16 gene as an example, that targeted demethylation of the promoter-exon 1-intron 1 (PrExI) region initiates an epigenetic wave of local chromatin remodeling and distal long-range interactions, culminating in gene-locus specific activation. Through development of CRISPR-DiR (DNMT1-interacting RNA), in which ad hoc edited guides block methyltransferase activity in a locus-specific fashion, we demonstrate that demethylation is coupled to epigenetic and topological changes. These results suggest the existence of a specialized “demethylation firing center (DFC)” which can be switched on by an adaptable and selective RNA-mediated approach for locus-specific transcriptional activation. One Sentence Summary Locus demethylation via CRISPR-DiR reshapes chromatin structure and specifically reactivates its cognate gene." @default.
- W3043205850 created "2020-07-23" @default.
- W3043205850 creator A5015800351 @default.
- W3043205850 creator A5022475557 @default.
- W3043205850 creator A5034956774 @default.
- W3043205850 creator A5051795758 @default.
- W3043205850 creator A5054735033 @default.
- W3043205850 creator A5056687452 @default.
- W3043205850 creator A5057599356 @default.
- W3043205850 creator A5058977705 @default.
- W3043205850 creator A5069595860 @default.
- W3043205850 creator A5071388367 @default.
- W3043205850 creator A5086617137 @default.
- W3043205850 creator A5089650704 @default.
- W3043205850 date "2020-07-17" @default.
- W3043205850 modified "2023-10-04" @default.
- W3043205850 title "Targeted intragenic demethylation initiates chromatin rewiring for gene activation" @default.
- W3043205850 cites W1504303645 @default.
- W3043205850 cites W1529905521 @default.
- W3043205850 cites W1643500256 @default.
- W3043205850 cites W1980051146 @default.
- W3043205850 cites W1984827465 @default.
- W3043205850 cites W2014677321 @default.
- W3043205850 cites W2029402659 @default.
- W3043205850 cites W2031842191 @default.
- W3043205850 cites W2039126025 @default.
- W3043205850 cites W2040257851 @default.
- W3043205850 cites W2043355615 @default.
- W3043205850 cites W2056495027 @default.
- W3043205850 cites W2075230442 @default.
- W3043205850 cites W2082540661 @default.
- W3043205850 cites W2098651180 @default.
- W3043205850 cites W2101749257 @default.
- W3043205850 cites W2102029982 @default.
- W3043205850 cites W2102619694 @default.
- W3043205850 cites W2108234281 @default.
- W3043205850 cites W2123032740 @default.
- W3043205850 cites W2123973342 @default.
- W3043205850 cites W2127132611 @default.
- W3043205850 cites W2127862779 @default.
- W3043205850 cites W2131374955 @default.
- W3043205850 cites W2133452708 @default.
- W3043205850 cites W2135169407 @default.
- W3043205850 cites W2137505881 @default.
- W3043205850 cites W2142515819 @default.
- W3043205850 cites W2170918883 @default.
- W3043205850 cites W2286869347 @default.
- W3043205850 cites W2336531194 @default.
- W3043205850 cites W2341539131 @default.
- W3043205850 cites W2416786266 @default.
- W3043205850 cites W2439189013 @default.
- W3043205850 cites W2551455897 @default.
- W3043205850 cites W2584914264 @default.
- W3043205850 cites W2754435174 @default.
- W3043205850 cites W2953219069 @default.
- W3043205850 cites W2965516429 @default.
- W3043205850 cites W2968658941 @default.
- W3043205850 cites W2973580032 @default.
- W3043205850 cites W2990785980 @default.
- W3043205850 cites W3125290545 @default.
- W3043205850 cites W4247053599 @default.
- W3043205850 doi "https://doi.org/10.1101/2020.07.16.205922" @default.
- W3043205850 hasPublicationYear "2020" @default.
- W3043205850 type Work @default.
- W3043205850 sameAs 3043205850 @default.
- W3043205850 citedByCount "4" @default.
- W3043205850 countsByYear W30432058502021 @default.
- W3043205850 countsByYear W30432058502023 @default.
- W3043205850 crossrefType "posted-content" @default.
- W3043205850 hasAuthorship W3043205850A5015800351 @default.
- W3043205850 hasAuthorship W3043205850A5022475557 @default.
- W3043205850 hasAuthorship W3043205850A5034956774 @default.
- W3043205850 hasAuthorship W3043205850A5051795758 @default.
- W3043205850 hasAuthorship W3043205850A5054735033 @default.
- W3043205850 hasAuthorship W3043205850A5056687452 @default.
- W3043205850 hasAuthorship W3043205850A5057599356 @default.
- W3043205850 hasAuthorship W3043205850A5058977705 @default.
- W3043205850 hasAuthorship W3043205850A5069595860 @default.
- W3043205850 hasAuthorship W3043205850A5071388367 @default.
- W3043205850 hasAuthorship W3043205850A5086617137 @default.
- W3043205850 hasAuthorship W3043205850A5089650704 @default.
- W3043205850 hasBestOaLocation W30432058501 @default.
- W3043205850 hasConcept C104317684 @default.
- W3043205850 hasConcept C119056186 @default.
- W3043205850 hasConcept C150194340 @default.
- W3043205850 hasConcept C167227067 @default.
- W3043205850 hasConcept C190727270 @default.
- W3043205850 hasConcept C202199734 @default.
- W3043205850 hasConcept C41091548 @default.
- W3043205850 hasConcept C54355233 @default.
- W3043205850 hasConcept C83640560 @default.
- W3043205850 hasConcept C84597430 @default.
- W3043205850 hasConcept C86803240 @default.
- W3043205850 hasConceptScore W3043205850C104317684 @default.
- W3043205850 hasConceptScore W3043205850C119056186 @default.
- W3043205850 hasConceptScore W3043205850C150194340 @default.
- W3043205850 hasConceptScore W3043205850C167227067 @default.
- W3043205850 hasConceptScore W3043205850C190727270 @default.
- W3043205850 hasConceptScore W3043205850C202199734 @default.
- W3043205850 hasConceptScore W3043205850C41091548 @default.