FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3043257292> ?p ?o ?g. }

• W3043257292 endingPage "112639" @default.
• W3043257292 startingPage "112639" @default.
• W3043257292 abstract "Ficellomycin is an aziridine-containing antibiotic, produced by Streptomyces ficellus. Based on the newly identified ficellomycin gene cluster and the assigned functions of its genes, a possible pathway for aziridine ring formation in ficellomycin was proposed, which is a complex process involving at least 3 enzymatic steps. To obtain support for the proposed mechanism, the targeted genes encoding sulfate adenylyltransferase, adenylsulfate kinase, and a putative sulfotransferase were respectively disrupted and the subsequent analysis of their fermentation products revealed that all the three genes were involved in aziridine formation. To further confirm the mechanism, the key gene encoding a putative sulfotransferase was over expressed in Escherichia coli Rosseta (DE3). Enzyme assays indicated that the expressed sulfotransferase could specifically transfer a sulfo group from 3′-phosphoadenosine-5′-phosphosulfate (PAPS) onto the hydroxyl group of (R)-(−)-2-pyrrolidinemethanol. This introduces a good leaving group in the form of the sulfated hydroxyl moiety, which is then converted into an aziridine ring through an intramolecular nucleophilic attack by the adjacent secondary amine. The sulfation/intramolecular cyclization reaction sequence maybe a general strategy for aziridine biosynthesis in microorganisms. Discovery of this mechanism revealed an enzyme-catalyzed route for the synthesis of aziridine-containing reagents and provided an important insight into the functional diversity of sulfotransferases." @default.
• W3043257292 created "2020-07-23" @default.
• W3043257292 creator A5015356629 @default.
• W3043257292 creator A5017947172 @default.
• W3043257292 creator A5031084065 @default.
• W3043257292 creator A5033632697 @default.
• W3043257292 creator A5060741317 @default.
• W3043257292 creator A5069302790 @default.
• W3043257292 creator A5078500829 @default.
• W3043257292 creator A5080081812 @default.
• W3043257292 creator A5087592842 @default.
• W3043257292 date "2020-10-01" @default.
• W3043257292 modified "2023-09-26" @default.
• W3043257292 title "Insight into enzyme-catalyzed aziridine formation mechanism in ficellomycin biosynthesis" @default.
• W3043257292 cites W1978535965 @default.
• W3043257292 cites W1986670170 @default.
• W3043257292 cites W1988070315 @default.
• W3043257292 cites W1991639205 @default.
• W3043257292 cites W1992840623 @default.
• W3043257292 cites W1994332632 @default.
• W3043257292 cites W2009810780 @default.
• W3043257292 cites W2009956300 @default.
• W3043257292 cites W2010359858 @default.
• W3043257292 cites W2017035988 @default.
• W3043257292 cites W2025992880 @default.
• W3043257292 cites W2029257339 @default.
• W3043257292 cites W2030849279 @default.
• W3043257292 cites W2030947759 @default.
• W3043257292 cites W2036333800 @default.
• W3043257292 cites W2052603675 @default.
• W3043257292 cites W2053804592 @default.
• W3043257292 cites W2056074643 @default.
• W3043257292 cites W2065249752 @default.
• W3043257292 cites W2069200567 @default.
• W3043257292 cites W2085927993 @default.
• W3043257292 cites W2090743507 @default.
• W3043257292 cites W2105742274 @default.
• W3043257292 cites W2131019078 @default.
• W3043257292 cites W2134732744 @default.
• W3043257292 cites W2157270500 @default.
• W3043257292 cites W2167832449 @default.
• W3043257292 cites W2317917488 @default.
• W3043257292 cites W2318594221 @default.
• W3043257292 cites W2329615575 @default.
• W3043257292 cites W2581180674 @default.
• W3043257292 cites W2755812181 @default.
• W3043257292 cites W2795358780 @default.
• W3043257292 cites W4229703458 @default.
• W3043257292 doi "https://doi.org/10.1016/j.ejmech.2020.112639" @default.
• W3043257292 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32712437" @default.
• W3043257292 hasPublicationYear "2020" @default.
• W3043257292 type Work @default.
• W3043257292 sameAs 3043257292 @default.
• W3043257292 citedByCount "6" @default.
• W3043257292 countsByYear W30432572922021 @default.
• W3043257292 countsByYear W30432572922022 @default.
• W3043257292 countsByYear W30432572922023 @default.
• W3043257292 crossrefType "journal-article" @default.
• W3043257292 hasAuthorship W3043257292A5015356629 @default.
• W3043257292 hasAuthorship W3043257292A5017947172 @default.
• W3043257292 hasAuthorship W3043257292A5031084065 @default.
• W3043257292 hasAuthorship W3043257292A5033632697 @default.
• W3043257292 hasAuthorship W3043257292A5060741317 @default.
• W3043257292 hasAuthorship W3043257292A5069302790 @default.
• W3043257292 hasAuthorship W3043257292A5078500829 @default.
• W3043257292 hasAuthorship W3043257292A5080081812 @default.
• W3043257292 hasAuthorship W3043257292A5087592842 @default.
• W3043257292 hasConcept C155138218 @default.
• W3043257292 hasConcept C178790620 @default.
• W3043257292 hasConcept C181199279 @default.
• W3043257292 hasConcept C185592680 @default.
• W3043257292 hasConcept C2775904581 @default.
• W3043257292 hasConcept C2778544067 @default.
• W3043257292 hasConcept C2780378348 @default.
• W3043257292 hasConcept C553450214 @default.
• W3043257292 hasConcept C55493867 @default.
• W3043257292 hasConcept C71240020 @default.
• W3043257292 hasConceptScore W3043257292C155138218 @default.
• W3043257292 hasConceptScore W3043257292C178790620 @default.
• W3043257292 hasConceptScore W3043257292C181199279 @default.
• W3043257292 hasConceptScore W3043257292C185592680 @default.
• W3043257292 hasConceptScore W3043257292C2775904581 @default.
• W3043257292 hasConceptScore W3043257292C2778544067 @default.
• W3043257292 hasConceptScore W3043257292C2780378348 @default.
• W3043257292 hasConceptScore W3043257292C553450214 @default.
• W3043257292 hasConceptScore W3043257292C55493867 @default.
• W3043257292 hasConceptScore W3043257292C71240020 @default.
• W3043257292 hasFunder F4320321001 @default.
• W3043257292 hasFunder F4320323993 @default.
• W3043257292 hasLocation W30432572921 @default.
• W3043257292 hasOpenAccess W3043257292 @default.
• W3043257292 hasPrimaryLocation W30432572921 @default.
• W3043257292 hasRelatedWork W1975340112 @default.
• W3043257292 hasRelatedWork W2000259043 @default.
• W3043257292 hasRelatedWork W2063679949 @default.
• W3043257292 hasRelatedWork W2273202453 @default.
• W3043257292 hasRelatedWork W2739100764 @default.
• W3043257292 hasRelatedWork W2886656117 @default.

• next