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• W3043288438 abstract "Growing cells is the typical mode of operation in many aspects of biotechnology and metabolic engineering. This comes about due to cell growth processes creating a driving force that pull metabolic flux along different metabolic pathways, that indirectly help move substrate to product. But, there is an alternative mode of operation that uses resting (non-growing) cells to achieve similar or even higher productivities. In general, resting cells are provided with carbon substrates for biocatalytic reactions but starved of nitrogen or phosphorus. Such resting cells have been usefully employed in many forms of biocatalysis and biotransformation, with or without cofactor regeneration. However, much remains unknown about the transcriptome and metabolome of resting cells in biotransformation settings. This short writeup provides the backdrop of resting cells in biocatalysis, documents their use in biotransformation with application examples, and identifies research gaps that could be filled with contemporary RNA-seq and mass spectrometry proteomics technology. Overall, utility of resting cells in biocatalysis and the extant knowledge gap in their fundamental physiology are highlighted in this resource." @default.
• W3043288438 created "2020-07-23" @default.
• W3043288438 creator A5004074519 @default.
• W3043288438 date "2020-07-17" @default.
• W3043288438 modified "2023-10-16" @default.
• W3043288438 title "Metabolism of Non-growing Cells and Their Application in Biotransformation" @default.
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• W3043288438 doi "https://doi.org/10.20944/preprints202007.0402.v1" @default.
• W3043288438 hasPublicationYear "2020" @default.
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