Matches in SemOpenAlex for { <https://semopenalex.org/work/W3043469558> ?p ?o ?g. }
- W3043469558 endingPage "9128" @default.
- W3043469558 startingPage "9115" @default.
- W3043469558 abstract "Eight related alcohol dehydrogenases, which had been originally isolated by laboratory evolution of ADH-A from Rhodococcus ruber DSM44541 for modified substrate scopes, were together with their parent wild-type subjected to biochemical characterization of possible activities with a panel of chiral alcohols and pro-chiral ketones. Determinations of rates of catalyzed alcohol oxidations and ketone reductions, and of cofactor release, pointed out to the role of a W295A substitution as being decisive in steering enantioselectivity in the oxidation of arylated 1-methyl substituted alcohols. Molecular dynamics simulations of enzyme–substrate interactions in the Michaelis complexes of wild-type ADH-A and a Y294F/W295A double mutant could rationalize the experimentally observed shift in enantioselectivity and differences in catalytic activity with 4-phenyl-2-butanol. Finally, we present herein evidence for apparent interdependency between substrate/product and the cofactor in the ternary complex, which directly affects the NADH dissociation rates; therefore, this substrate-coenzyme crosstalk plays a direct role in determining the turnover rates." @default.
- W3043469558 created "2020-07-23" @default.
- W3043469558 creator A5033662600 @default.
- W3043469558 creator A5067743846 @default.
- W3043469558 creator A5076761253 @default.
- W3043469558 creator A5079060690 @default.
- W3043469558 date "2020-07-16" @default.
- W3043469558 modified "2023-10-16" @default.
- W3043469558 title "The Role of Substrate-Coenzyme Crosstalk in Determining Turnover Rates in <i>Rhodococcus ruber</i> Alcohol Dehydrogenase" @default.
- W3043469558 cites W158661334 @default.
- W3043469558 cites W1757079284 @default.
- W3043469558 cites W1954202239 @default.
- W3043469558 cites W1969335533 @default.
- W3043469558 cites W1969492379 @default.
- W3043469558 cites W1970968922 @default.
- W3043469558 cites W1976499671 @default.
- W3043469558 cites W1976836655 @default.
- W3043469558 cites W1984180361 @default.
- W3043469558 cites W1986056153 @default.
- W3043469558 cites W1986361066 @default.
- W3043469558 cites W1987696643 @default.
- W3043469558 cites W1987818912 @default.
- W3043469558 cites W1996054932 @default.
- W3043469558 cites W2000498353 @default.
- W3043469558 cites W2003620627 @default.
- W3043469558 cites W2015561777 @default.
- W3043469558 cites W2015688756 @default.
- W3043469558 cites W2016025162 @default.
- W3043469558 cites W2018154356 @default.
- W3043469558 cites W2019051049 @default.
- W3043469558 cites W2021150166 @default.
- W3043469558 cites W2029961407 @default.
- W3043469558 cites W2031117172 @default.
- W3043469558 cites W2035266068 @default.
- W3043469558 cites W2038555855 @default.
- W3043469558 cites W2038758364 @default.
- W3043469558 cites W2062239628 @default.
- W3043469558 cites W2063597962 @default.
- W3043469558 cites W2077680621 @default.
- W3043469558 cites W2086361102 @default.
- W3043469558 cites W2106140689 @default.
- W3043469558 cites W2110198171 @default.
- W3043469558 cites W2113723064 @default.
- W3043469558 cites W2114842134 @default.
- W3043469558 cites W2119414767 @default.
- W3043469558 cites W2125712194 @default.
- W3043469558 cites W2128312954 @default.
- W3043469558 cites W2134441341 @default.
- W3043469558 cites W2135900956 @default.
- W3043469558 cites W2142363369 @default.
- W3043469558 cites W2142623223 @default.
- W3043469558 cites W2147993766 @default.
- W3043469558 cites W2148612893 @default.
- W3043469558 cites W2149263899 @default.
- W3043469558 cites W2152075687 @default.
- W3043469558 cites W2160719116 @default.
- W3043469558 cites W2242726375 @default.
- W3043469558 cites W2285619533 @default.
- W3043469558 cites W2328198700 @default.
- W3043469558 cites W2330799739 @default.
- W3043469558 cites W2400423509 @default.
- W3043469558 cites W2404280981 @default.
- W3043469558 cites W2512259981 @default.
- W3043469558 cites W2566018625 @default.
- W3043469558 cites W2571341234 @default.
- W3043469558 cites W2606937441 @default.
- W3043469558 cites W2611358696 @default.
- W3043469558 cites W2613083880 @default.
- W3043469558 cites W2735208100 @default.
- W3043469558 cites W2737721824 @default.
- W3043469558 cites W2743269523 @default.
- W3043469558 cites W2787148031 @default.
- W3043469558 cites W2875972510 @default.
- W3043469558 cites W2951925237 @default.
- W3043469558 cites W2952706713 @default.
- W3043469558 cites W4239792147 @default.
- W3043469558 cites W4255677297 @default.
- W3043469558 doi "https://doi.org/10.1021/acscatal.0c01654" @default.
- W3043469558 hasPublicationYear "2020" @default.
- W3043469558 type Work @default.
- W3043469558 sameAs 3043469558 @default.
- W3043469558 citedByCount "7" @default.
- W3043469558 countsByYear W30434695582020 @default.
- W3043469558 countsByYear W30434695582021 @default.
- W3043469558 countsByYear W30434695582022 @default.
- W3043469558 countsByYear W30434695582023 @default.
- W3043469558 crossrefType "journal-article" @default.
- W3043469558 hasAuthorship W3043469558A5033662600 @default.
- W3043469558 hasAuthorship W3043469558A5067743846 @default.
- W3043469558 hasAuthorship W3043469558A5076761253 @default.
- W3043469558 hasAuthorship W3043469558A5079060690 @default.
- W3043469558 hasBestOaLocation W30434695581 @default.
- W3043469558 hasConcept C161790260 @default.
- W3043469558 hasConcept C181199279 @default.
- W3043469558 hasConcept C185592680 @default.
- W3043469558 hasConcept C18903297 @default.
- W3043469558 hasConcept C191503008 @default.
- W3043469558 hasConcept C197957613 @default.