FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3043505325> ?p ?o ?g. }

• W3043505325 abstract "Abstract In neuronal exocytosis, SNARE assembly into a stable four-helix bundle drives membrane fusion. Previous studies have revealed that the SM protein Munc18-1 plays a critical role for precise SNARE assembly with the help of Munc13-1, but the underlying mechanism remains unclear. Here, we used single-molecule FRET assays with a nanodisc membrane reconstitution system to investigate the conformational dynamics of SNARE/Munc18-1 complexes in multiple intermediate steps towards the SNARE complex. We found that single Munc18-1 proteins induce the closed conformation of syntaxin-1 not only in the free syntaxin-1 but also in the t-SNARE (syntaxin-1/SNAP-25) complex. These results implicate that Munc18-1 may act as a gatekeeper for both binary and ternary SNARE complex formation by locking the syntaxin-1 in a cleft of Munc18-1. Furthermore, the kinetic analysis of the opening/closing transition reveals that the closed syntaxin-1 in the syntaxin-1/SNAP-25/Munc18-1 complex is less stable than that in the closed syntaxin-1/Munc18-1 complex, which is manifested by the infrequent closing transition, indicating that the conformational equilibrium of the ternary complex is biased toward the open conformation of syntaxin-1 compared with the binary complex." @default.
• W3043505325 created "2020-07-23" @default.
• W3043505325 creator A5007579590 @default.
• W3043505325 creator A5046827465 @default.
• W3043505325 creator A5051046735 @default.
• W3043505325 creator A5063141275 @default.
• W3043505325 creator A5064410082 @default.
• W3043505325 creator A5067749153 @default.
• W3043505325 creator A5083190409 @default.
• W3043505325 creator A5088996540 @default.
• W3043505325 date "2020-07-15" @default.
• W3043505325 modified "2023-10-14" @default.
• W3043505325 title "Munc18-1 induces conformational changes of syntaxin-1 in multiple intermediates for SNARE assembly" @default.
• W3043505325 cites W1497380575 @default.
• W3043505325 cites W1822005186 @default.
• W3043505325 cites W1822252127 @default.
• W3043505325 cites W1978098224 @default.
• W3043505325 cites W1999213641 @default.
• W3043505325 cites W2000302106 @default.
• W3043505325 cites W2013617213 @default.
• W3043505325 cites W2024136850 @default.
• W3043505325 cites W2024251756 @default.
• W3043505325 cites W2031212561 @default.
• W3043505325 cites W2033788106 @default.
• W3043505325 cites W2048465987 @default.
• W3043505325 cites W2061387029 @default.
• W3043505325 cites W2067354100 @default.
• W3043505325 cites W2076220928 @default.
• W3043505325 cites W2086919645 @default.
• W3043505325 cites W2094779328 @default.
• W3043505325 cites W2098744542 @default.
• W3043505325 cites W2101097180 @default.
• W3043505325 cites W2120360272 @default.
• W3043505325 cites W2127017351 @default.
• W3043505325 cites W2128978059 @default.
• W3043505325 cites W2142844453 @default.
• W3043505325 cites W2153569460 @default.
• W3043505325 cites W2159529647 @default.
• W3043505325 cites W2166747217 @default.
• W3043505325 cites W2235178287 @default.
• W3043505325 cites W2272549607 @default.
• W3043505325 cites W2441542994 @default.
• W3043505325 cites W2581328787 @default.
• W3043505325 cites W2612109171 @default.
• W3043505325 cites W2741524102 @default.
• W3043505325 cites W2910595951 @default.
• W3043505325 cites W2951496883 @default.
• W3043505325 cites W2996914143 @default.
• W3043505325 doi "https://doi.org/10.1038/s41598-020-68476-3" @default.
• W3043505325 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7363831" @default.
• W3043505325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32669573" @default.
• W3043505325 hasPublicationYear "2020" @default.
• W3043505325 type Work @default.
• W3043505325 sameAs 3043505325 @default.
• W3043505325 citedByCount "8" @default.
• W3043505325 countsByYear W30435053252021 @default.
• W3043505325 countsByYear W30435053252022 @default.
• W3043505325 countsByYear W30435053252023 @default.
• W3043505325 crossrefType "journal-article" @default.
• W3043505325 hasAuthorship W3043505325A5007579590 @default.
• W3043505325 hasAuthorship W3043505325A5046827465 @default.
• W3043505325 hasAuthorship W3043505325A5051046735 @default.
• W3043505325 hasAuthorship W3043505325A5063141275 @default.
• W3043505325 hasAuthorship W3043505325A5064410082 @default.
• W3043505325 hasAuthorship W3043505325A5067749153 @default.
• W3043505325 hasAuthorship W3043505325A5083190409 @default.
• W3043505325 hasAuthorship W3043505325A5088996540 @default.
• W3043505325 hasBestOaLocation W30435053251 @default.
• W3043505325 hasConcept C103038307 @default.
• W3043505325 hasConcept C12554922 @default.
• W3043505325 hasConcept C130316041 @default.
• W3043505325 hasConcept C148785051 @default.
• W3043505325 hasConcept C175969161 @default.
• W3043505325 hasConcept C181199279 @default.
• W3043505325 hasConcept C185592680 @default.
• W3043505325 hasConcept C2778256703 @default.
• W3043505325 hasConcept C2908790754 @default.
• W3043505325 hasConcept C41625074 @default.
• W3043505325 hasConcept C53728453 @default.
• W3043505325 hasConcept C55493867 @default.
• W3043505325 hasConcept C65972015 @default.
• W3043505325 hasConcept C79061702 @default.
• W3043505325 hasConcept C86756495 @default.
• W3043505325 hasConcept C86803240 @default.
• W3043505325 hasConcept C95444343 @default.
• W3043505325 hasConceptScore W3043505325C103038307 @default.
• W3043505325 hasConceptScore W3043505325C12554922 @default.
• W3043505325 hasConceptScore W3043505325C130316041 @default.
• W3043505325 hasConceptScore W3043505325C148785051 @default.
• W3043505325 hasConceptScore W3043505325C175969161 @default.
• W3043505325 hasConceptScore W3043505325C181199279 @default.
• W3043505325 hasConceptScore W3043505325C185592680 @default.
• W3043505325 hasConceptScore W3043505325C2778256703 @default.
• W3043505325 hasConceptScore W3043505325C2908790754 @default.
• W3043505325 hasConceptScore W3043505325C41625074 @default.
• W3043505325 hasConceptScore W3043505325C53728453 @default.
• W3043505325 hasConceptScore W3043505325C55493867 @default.
• W3043505325 hasConceptScore W3043505325C65972015 @default.
• W3043505325 hasConceptScore W3043505325C79061702 @default.
• W3043505325 hasConceptScore W3043505325C86756495 @default.

• next