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• W3043650295 startingPage "112829" @default.
• W3043650295 abstract "To evaluate the analytical performance of our previously developed chemiluminescence immunoassay (CLIA) kit for the detection of procalcitonin (PCT) and compare with the results obtained using the Vidas B.R.A.H.M.S. PCT™ test (PCT-V). Our laboratory previously designed a novel CLIA kit and supporting instrument (AE-180) for the detection of PCT. We analyzed the clinical performance of this system, including the imprecision, limit of detection, and linearity of analyses of 305 serum specimens. The results were compared with measurements of the same serum samples obtained with PCT-V. The limit of detection and blank of our kit were 0.0075 and 0.0039 ng/mL, respectively. The intra- and inter-assay coefficient of variation of the kit were both between 0.8% and 3.9%. The equation of linearity was found to be y = 1.03× + 0.06 (r = 0.99) for concentrations in the range of 0.01–110 ng/mL. The correlation coefficient with the results of PCT-V was 0.995, and the equation obtained for Passing and Bablok regression analysis was 1.061 for our CLIA PCT kit and − 0.003 for PCT-V. Our kit slightly overestimated the concentration according to comparison with PCT-V results. The kit that was previously developed in our laboratory for the measurement of serum PCT concentration using CLIA technology shows excellent performance, just that the functional sensitivity is not as good as the PCT-V; therefore, we suggest that this kit is suitable for clinical use." @default.
• W3043650295 created "2020-07-23" @default.
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• W3043650295 date "2020-09-01" @default.
• W3043650295 modified "2023-10-15" @default.
• W3043650295 title "Development of a novel chemiluminescence immunoassay for the detection of procalcitonin" @default.
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• W3043650295 doi "https://doi.org/10.1016/j.jim.2020.112829" @default.
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