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• W3043689506 abstract "Light-sheet microscopy (LSM) has emerged as a powerful tool for high-speed volumetric imaging of live model organisms and large optically cleared specimens. When designing cleared-tissue LSM systems with certain desired imaging specifications (e.g. resolution, contrast, and working distance), various design parameters must be taken into consideration. In order to elucidate some of the key design tradeoffs for LSM systems, we present a diffraction-based analysis of single- and dual-objective LSM configurations using simulations of LSM point spread functions. We assume Gaussian illumination is utilized. Specifically, we analyze the effects of the illumination and collection numerical aperture (NA), as well as their crossing angle, on spatial resolution and contrast. Assuming an open-top light-sheet (OTLS) architecture, we constrain these parameters based on fundamental geometric considerations as well as those imposed by currently available microscope objectives. In addition to revealing the performance tradeoffs of various single- and dual-objective LSM configurations, our analysis showcases the potential advantages of a novel, non-orthogonal dual-objective (NODO) architecture, especially for moderate-resolution imaging applications (collection NA of 0.5 to 0.8)." @default.
• W3043689506 created "2020-07-23" @default.
• W3043689506 creator A5014254460 @default.
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• W3043689506 date "2020-07-24" @default.
• W3043689506 modified "2023-10-11" @default.
• W3043689506 title "Performance tradeoffs for single- and dual-objective open-top light-sheet microscope designs: a simulation-based analysis" @default.
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• W3043689506 doi "https://doi.org/10.1364/boe.397052" @default.
• W3043689506 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7449713" @default.
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• W3043689506 hasPublicationYear "2020" @default.
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