FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044015075> ?p ?o ?g. }

• W3044015075 endingPage "158768" @default.
• W3044015075 startingPage "158768" @default.
• W3044015075 abstract "Phosphatidic acid (PA) consists of various molecular species that have different fatty acyl chains at the sn-1 and sn-2 positions; and consequently, mammalian cells contain at least 50 structurally distinct PA molecular species. However, the different roles of each PA species are poorly understood. In the present study, we attempted to identify dipalmitoyl (16:0/16:0)-PA-binding proteins from mouse skeletal muscle using liposome precipitation and tandem mass spectrometry analysis. We identified L-lactate dehydrogenase (LDH) A, which catalyzes conversion of pyruvate to lactate and is a key checkpoint of anaerobic glycolysis critical for tumor growth, as a 16:0/16:0-PA-binding protein. LDHA did not substantially associate with other phospholipids, such as phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, phosphoinositides and cardiolipin at physiological pH (7.4), indicating that LDHA specifically bound to PA. Interestingly, 18:0/18:0-, 18:0/20:4- and 18:0/22:6-PA also interacted with LDHA, and their binding activities were stronger than 16:0/16:0-PA at pH 7.4. Moreover, circular dichroism spectrometry showed that 18:0/20:4- and 18:0/22:6-PA, but not 16:0/16:0- or 18:0/18:0-PA, significantly reduced the α-helical structure of LDHA. Furthermore, 18:0/20:4- and 18:0/22:6-PA attenuated LDH activity. Taken together, we demonstrated for the first time that LDHA is a PA-binding protein and is a unique PA-binding protein that is structurally and functionally controlled by associating with 18:0/20:4- and 18:0/22:6-PA." @default.
• W3044015075 created "2020-07-29" @default.
• W3044015075 creator A5018843945 @default.
• W3044015075 creator A5052438029 @default.
• W3044015075 date "2020-10-01" @default.
• W3044015075 modified "2023-09-25" @default.
• W3044015075 title "Polyunsaturated fatty acid-containing phosphatidic acids selectively interact with L-lactate dehydrogenase A and induce its secondary structural change and inactivation" @default.
• W3044015075 cites W1486424890 @default.
• W3044015075 cites W1544573343 @default.
• W3044015075 cites W1920706136 @default.
• W3044015075 cites W1935974026 @default.
• W3044015075 cites W1965748088 @default.
• W3044015075 cites W1965881649 @default.
• W3044015075 cites W1967385667 @default.
• W3044015075 cites W1982501876 @default.
• W3044015075 cites W1986486541 @default.
• W3044015075 cites W1988865934 @default.
• W3044015075 cites W1992117451 @default.
• W3044015075 cites W1992413985 @default.
• W3044015075 cites W1993159760 @default.
• W3044015075 cites W1995635092 @default.
• W3044015075 cites W1995763262 @default.
• W3044015075 cites W2001766446 @default.
• W3044015075 cites W2013706577 @default.
• W3044015075 cites W2025938842 @default.
• W3044015075 cites W2026653301 @default.
• W3044015075 cites W2033131480 @default.
• W3044015075 cites W2035998354 @default.
• W3044015075 cites W2044921355 @default.
• W3044015075 cites W2054573680 @default.
• W3044015075 cites W2055289589 @default.
• W3044015075 cites W2056801079 @default.
• W3044015075 cites W2057757186 @default.
• W3044015075 cites W2059672809 @default.
• W3044015075 cites W2060611693 @default.
• W3044015075 cites W2064262345 @default.
• W3044015075 cites W2066159172 @default.
• W3044015075 cites W2068050569 @default.
• W3044015075 cites W2075173556 @default.
• W3044015075 cites W2077907202 @default.
• W3044015075 cites W2079724447 @default.
• W3044015075 cites W2083719876 @default.
• W3044015075 cites W2084809668 @default.
• W3044015075 cites W2085753216 @default.
• W3044015075 cites W2086850937 @default.
• W3044015075 cites W2087700852 @default.
• W3044015075 cites W2094961458 @default.
• W3044015075 cites W2097259163 @default.
• W3044015075 cites W2101246344 @default.
• W3044015075 cites W2109195162 @default.
• W3044015075 cites W2111525938 @default.
• W3044015075 cites W2112239959 @default.
• W3044015075 cites W2115769774 @default.
• W3044015075 cites W2117953915 @default.
• W3044015075 cites W2120621064 @default.
• W3044015075 cites W2130323165 @default.
• W3044015075 cites W2135890751 @default.
• W3044015075 cites W2136090166 @default.
• W3044015075 cites W2140260269 @default.
• W3044015075 cites W2143320896 @default.
• W3044015075 cites W2163471981 @default.
• W3044015075 cites W2164362108 @default.
• W3044015075 cites W2166817392 @default.
• W3044015075 cites W2281848173 @default.
• W3044015075 cites W2303572581 @default.
• W3044015075 cites W2312774930 @default.
• W3044015075 cites W2351568661 @default.
• W3044015075 cites W2461823623 @default.
• W3044015075 cites W2511443137 @default.
• W3044015075 cites W2521280158 @default.
• W3044015075 cites W2580097497 @default.
• W3044015075 cites W2587752484 @default.
• W3044015075 cites W2739343101 @default.
• W3044015075 cites W2753406012 @default.
• W3044015075 cites W2792142152 @default.
• W3044015075 cites W2885458606 @default.
• W3044015075 cites W2889229834 @default.
• W3044015075 cites W2903631224 @default.
• W3044015075 cites W2936485785 @default.
• W3044015075 cites W2943929191 @default.
• W3044015075 cites W2987848499 @default.
• W3044015075 cites W3009673279 @default.
• W3044015075 cites W3010726049 @default.
• W3044015075 doi "https://doi.org/10.1016/j.bbalip.2020.158768" @default.
• W3044015075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32717303" @default.
• W3044015075 hasPublicationYear "2020" @default.
• W3044015075 type Work @default.
• W3044015075 sameAs 3044015075 @default.
• W3044015075 citedByCount "6" @default.
• W3044015075 countsByYear W30440150752020 @default.
• W3044015075 countsByYear W30440150752021 @default.
• W3044015075 countsByYear W30440150752022 @default.
• W3044015075 crossrefType "journal-article" @default.
• W3044015075 hasAuthorship W3044015075A5018843945 @default.
• W3044015075 hasAuthorship W3044015075A5052438029 @default.
• W3044015075 hasConcept C133571119 @default.
• W3044015075 hasConcept C181199279 @default.
• W3044015075 hasConcept C185592680 @default.

• next