Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044054966> ?p ?o ?g. }
- W3044054966 endingPage "107739" @default.
- W3044054966 startingPage "107739" @default.
- W3044054966 abstract "Mycobacteria is important microbial cell factory in the steroid industry, and there is an urgent need to develop synthetic biology toolkit to regulate the expression level of genes in mycobacterial strains. In this study, we first evaluated the adaptation of seven promoters in Mycobacterium neoaurum ATCC 25795, including trp, tac, trc, and tic from Escherichia coli, pm2 from Bacillus subtilis, and hsp60, hsp70 from Mycobacterium. In order to obtain stronger promoters, 10 new artificial promoters were obtained by retaining the core regions with appropriate truncation, and by concatenating these promoters to increase the sigma factor recognition region. Secondly, we constructed a ribosomal binding sites (RBS) library (with 49 RBSs) based on the RBS Calculator-predicted and iGEM design, and verified their regulation on genes expression in Mycobacterium. Since AU-rich sequences increase mRNA stability and/or translation efficiency, we obtained 38 strong RBS and 11 weak RBS compared to the control (the RBS of the plasmid pMV261). Eventually, we chose a high-strength promoter (CP6) and a medium-strength RBS (R1) to drive the overexpression of the genes encoding 3-ketosteroid-9α-hydroxylase (kshA and kshB) in M. neoaurum, leading to a production titer of 9α-hydroxy-androst-4-ene-3,17-dione (9-OHAD) of 5.72 ± 0.05 g/L (with 13.0 g/L phytosterols as the substrates), a significant increase in comparison to the original strain (4.58 ± 0.02 g/L)." @default.
- W3044054966 created "2020-07-29" @default.
- W3044054966 creator A5030519104 @default.
- W3044054966 creator A5077155551 @default.
- W3044054966 creator A5081040608 @default.
- W3044054966 date "2020-10-01" @default.
- W3044054966 modified "2023-10-13" @default.
- W3044054966 title "Engineering mycobacteria artificial promoters and ribosomal binding sites for enhanced sterol production" @default.
- W3044054966 cites W1528872009 @default.
- W3044054966 cites W1559169404 @default.
- W3044054966 cites W1708105888 @default.
- W3044054966 cites W186350300 @default.
- W3044054966 cites W1941794513 @default.
- W3044054966 cites W1943504827 @default.
- W3044054966 cites W1968790539 @default.
- W3044054966 cites W1969989079 @default.
- W3044054966 cites W1976799792 @default.
- W3044054966 cites W1981580562 @default.
- W3044054966 cites W1981584314 @default.
- W3044054966 cites W1990951667 @default.
- W3044054966 cites W1994145955 @default.
- W3044054966 cites W1994800487 @default.
- W3044054966 cites W1995481544 @default.
- W3044054966 cites W2005109861 @default.
- W3044054966 cites W2015476615 @default.
- W3044054966 cites W2016233564 @default.
- W3044054966 cites W2016780222 @default.
- W3044054966 cites W2020366340 @default.
- W3044054966 cites W2028603053 @default.
- W3044054966 cites W2040432888 @default.
- W3044054966 cites W2042231801 @default.
- W3044054966 cites W2046737353 @default.
- W3044054966 cites W2047360542 @default.
- W3044054966 cites W2047510552 @default.
- W3044054966 cites W2053558244 @default.
- W3044054966 cites W2061410357 @default.
- W3044054966 cites W2061659241 @default.
- W3044054966 cites W2068017263 @default.
- W3044054966 cites W2076264079 @default.
- W3044054966 cites W2082806783 @default.
- W3044054966 cites W2107277218 @default.
- W3044054966 cites W2117593281 @default.
- W3044054966 cites W2118640842 @default.
- W3044054966 cites W2120100753 @default.
- W3044054966 cites W2123093125 @default.
- W3044054966 cites W2124374420 @default.
- W3044054966 cites W2129434797 @default.
- W3044054966 cites W2132963003 @default.
- W3044054966 cites W2134582538 @default.
- W3044054966 cites W2138143337 @default.
- W3044054966 cites W2147982990 @default.
- W3044054966 cites W2165427247 @default.
- W3044054966 cites W2170023904 @default.
- W3044054966 cites W2171993759 @default.
- W3044054966 cites W2277779396 @default.
- W3044054966 cites W2324591890 @default.
- W3044054966 cites W2554960318 @default.
- W3044054966 cites W2562950907 @default.
- W3044054966 cites W2586637020 @default.
- W3044054966 cites W2589298666 @default.
- W3044054966 cites W2768878520 @default.
- W3044054966 cites W2770591493 @default.
- W3044054966 cites W2804644735 @default.
- W3044054966 cites W2810074425 @default.
- W3044054966 cites W2810982945 @default.
- W3044054966 cites W2884086582 @default.
- W3044054966 cites W2912479692 @default.
- W3044054966 cites W2921451487 @default.
- W3044054966 cites W4236034745 @default.
- W3044054966 doi "https://doi.org/10.1016/j.bej.2020.107739" @default.
- W3044054966 hasPublicationYear "2020" @default.
- W3044054966 type Work @default.
- W3044054966 sameAs 3044054966 @default.
- W3044054966 citedByCount "11" @default.
- W3044054966 countsByYear W30440549662021 @default.
- W3044054966 countsByYear W30440549662022 @default.
- W3044054966 countsByYear W30440549662023 @default.
- W3044054966 crossrefType "journal-article" @default.
- W3044054966 hasAuthorship W3044054966A5030519104 @default.
- W3044054966 hasAuthorship W3044054966A5077155551 @default.
- W3044054966 hasAuthorship W3044054966A5081040608 @default.
- W3044054966 hasConcept C101762097 @default.
- W3044054966 hasConcept C104317684 @default.
- W3044054966 hasConcept C114847915 @default.
- W3044054966 hasConcept C145640855 @default.
- W3044054966 hasConcept C150194340 @default.
- W3044054966 hasConcept C153911025 @default.
- W3044054966 hasConcept C22744801 @default.
- W3044054966 hasConcept C2777272437 @default.
- W3044054966 hasConcept C2780374374 @default.
- W3044054966 hasConcept C40767141 @default.
- W3044054966 hasConcept C523546767 @default.
- W3044054966 hasConcept C54355233 @default.
- W3044054966 hasConcept C547475151 @default.
- W3044054966 hasConcept C67705224 @default.
- W3044054966 hasConcept C67905577 @default.
- W3044054966 hasConcept C86803240 @default.
- W3044054966 hasConcept C88478588 @default.