Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044149188> ?p ?o ?g. }
- W3044149188 abstract "In the last decades, the scientific community spared no effort to elucidate the therapeutic potential of mesenchymal stromal cells (MSCs). Unfortunately, in vitro cellular senescence occurring along with a loss of proliferative capacity is a major drawback in view of future therapeutic applications of these cells in the field of regenerative medicine. Even though insight into the mechanisms of replicative senescence in human medicine has evolved dramatically, knowledge about replicative senescence of canine MSCs is still scarce. Thus, we developed a high-content analysis workflow to simultaneously investigate three important characteristics of senescence in canine adipose-derived MSCs (cAD-MSCs): morphological changes, activation of the cell cycle arrest machinery and increased activity of the senescence-associated beta-galactosidase. We took advantage of this tool to demonstrate that passaging of cAD-MSCs results in the appearance of a senescence phenotype and proliferation arrest. This was partially prevented upon immortalization of these cells using a newly designed PiggyBac(TM) Transposon System, which allows for the expression of the human polycomb ring finger proto-oncogene BMI1 and the human telomerase reverse transcriptase under the same promotor. Our results indicate that cAD-MSCs immortalized with this new vector maintain their proliferation capacity and differentiation potential for a longer time than untreated cAD-MSCs. This study not only offers a workflow to investigate replicative senescence in eukaryotic cells with a high-content analysis approach but also paves the way for a rapid and effective generation of immortalized MSC lines. This promotes a better understanding of these cells in view of future applications in regenerative medicine." @default.
- W3044149188 created "2020-07-29" @default.
- W3044149188 creator A5024572016 @default.
- W3044149188 creator A5026101071 @default.
- W3044149188 creator A5031417486 @default.
- W3044149188 creator A5049539404 @default.
- W3044149188 creator A5061116391 @default.
- W3044149188 creator A5075477708 @default.
- W3044149188 date "2020-07-27" @default.
- W3044149188 modified "2023-09-25" @default.
- W3044149188 title "High-content analysis reveals senescence of cultured canine adipose-derived mesenchymal stromal cells to be reversible with a novel immortalization approach" @default.
- W3044149188 cites W1758822526 @default.
- W3044149188 cites W1914290020 @default.
- W3044149188 cites W1965041833 @default.
- W3044149188 cites W1970924670 @default.
- W3044149188 cites W1984936995 @default.
- W3044149188 cites W1990419270 @default.
- W3044149188 cites W1992165517 @default.
- W3044149188 cites W1995205742 @default.
- W3044149188 cites W1995276190 @default.
- W3044149188 cites W2007416316 @default.
- W3044149188 cites W2022322309 @default.
- W3044149188 cites W2025371271 @default.
- W3044149188 cites W2038454445 @default.
- W3044149188 cites W2049446122 @default.
- W3044149188 cites W2050742889 @default.
- W3044149188 cites W2066852179 @default.
- W3044149188 cites W2072527270 @default.
- W3044149188 cites W2075881401 @default.
- W3044149188 cites W2080961798 @default.
- W3044149188 cites W2082144896 @default.
- W3044149188 cites W2083256869 @default.
- W3044149188 cites W2110934787 @default.
- W3044149188 cites W2117653463 @default.
- W3044149188 cites W2140251048 @default.
- W3044149188 cites W2146841456 @default.
- W3044149188 cites W2164005910 @default.
- W3044149188 cites W2167712338 @default.
- W3044149188 cites W2193686370 @default.
- W3044149188 cites W2204746834 @default.
- W3044149188 cites W2297680570 @default.
- W3044149188 cites W2403915156 @default.
- W3044149188 cites W2494515430 @default.
- W3044149188 cites W2504155943 @default.
- W3044149188 cites W2560890620 @default.
- W3044149188 cites W2582117034 @default.
- W3044149188 cites W2614375081 @default.
- W3044149188 cites W2617594540 @default.
- W3044149188 cites W2741732319 @default.
- W3044149188 cites W2766180797 @default.
- W3044149188 cites W2788548733 @default.
- W3044149188 cites W2790571094 @default.
- W3044149188 cites W2804527144 @default.
- W3044149188 cites W2808355836 @default.
- W3044149188 cites W2950854031 @default.
- W3044149188 cites W2960919705 @default.
- W3044149188 doi "https://doi.org/10.1101/2020.07.27.217596" @default.
- W3044149188 hasPublicationYear "2020" @default.
- W3044149188 type Work @default.
- W3044149188 sameAs 3044149188 @default.
- W3044149188 citedByCount "0" @default.
- W3044149188 crossrefType "posted-content" @default.
- W3044149188 hasAuthorship W3044149188A5024572016 @default.
- W3044149188 hasAuthorship W3044149188A5026101071 @default.
- W3044149188 hasAuthorship W3044149188A5031417486 @default.
- W3044149188 hasAuthorship W3044149188A5049539404 @default.
- W3044149188 hasAuthorship W3044149188A5061116391 @default.
- W3044149188 hasAuthorship W3044149188A5075477708 @default.
- W3044149188 hasBestOaLocation W30441491881 @default.
- W3044149188 hasConcept C104317684 @default.
- W3044149188 hasConcept C10854531 @default.
- W3044149188 hasConcept C127716648 @default.
- W3044149188 hasConcept C1491633281 @default.
- W3044149188 hasConcept C16930146 @default.
- W3044149188 hasConcept C198826908 @default.
- W3044149188 hasConcept C28328180 @default.
- W3044149188 hasConcept C29537977 @default.
- W3044149188 hasConcept C502942594 @default.
- W3044149188 hasConcept C522857546 @default.
- W3044149188 hasConcept C54355233 @default.
- W3044149188 hasConcept C6045943 @default.
- W3044149188 hasConcept C81885089 @default.
- W3044149188 hasConcept C86803240 @default.
- W3044149188 hasConcept C94581717 @default.
- W3044149188 hasConcept C95444343 @default.
- W3044149188 hasConceptScore W3044149188C104317684 @default.
- W3044149188 hasConceptScore W3044149188C10854531 @default.
- W3044149188 hasConceptScore W3044149188C127716648 @default.
- W3044149188 hasConceptScore W3044149188C1491633281 @default.
- W3044149188 hasConceptScore W3044149188C16930146 @default.
- W3044149188 hasConceptScore W3044149188C198826908 @default.
- W3044149188 hasConceptScore W3044149188C28328180 @default.
- W3044149188 hasConceptScore W3044149188C29537977 @default.
- W3044149188 hasConceptScore W3044149188C502942594 @default.
- W3044149188 hasConceptScore W3044149188C522857546 @default.
- W3044149188 hasConceptScore W3044149188C54355233 @default.
- W3044149188 hasConceptScore W3044149188C6045943 @default.
- W3044149188 hasConceptScore W3044149188C81885089 @default.
- W3044149188 hasConceptScore W3044149188C86803240 @default.
- W3044149188 hasConceptScore W3044149188C94581717 @default.