FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044221262> ?p ?o ?g. }

• W3044221262 endingPage "108081" @default.
• W3044221262 startingPage "108081" @default.
• W3044221262 abstract "Cellulase cocktails formulated to degrade crystalline cellulose generally contain cellobiohydrolases (CBHs), referred to as CBHI (Cel7A) and CBHII (Cel6A), as the major constituents. The combined hydrolytic activities of CBHI and CBHII improve the release of fermentable sugars (β-1,4-cellobiose as the main product) from crystalline cellulose. In this study, a novel cellobiohydrolase (Exg-D) sourced from a metagenome of hindgut bacterial symbionts of a termite was heterologouly expressed, purified, and functionally characterised. Exg-D specific activity was higher on insoluble barley β-glucan (38.94 U/mg protein), soluble wheat flour β-glucan (12.71 U/mg protein) and oat β-glucan (8.89 U/mg protein) compared to cellulosic substrates; Avicel and CMC. We further explored Exg-D activity on the unpretreated or NaOH-pretreated (mercerised) Avicel and compared its activity to commercially available CBHI and CBHII on these celluloses. CBHI displayed the highest activity of 4.74 U/mg protein on mercerised cellulose followed by CBHII (2.14 U/mg protein), while Exg-D activity on untreated and mercerised cellulose was 1.66 and 1.67 U/mg protein, respectively. The high activity of CBHI was supported by binding assays, which revealed that CBHI has a higher binding capacity towards crystalline cellulose compared to Exg-D and CBHII. Only CBHI and CBHII showed synergism during the hydrolysis of mercerised Avicel, showing a degree of synergy (DS) of about 1.299 and yielded about 1.43 μmol/ml of reducing sugars higher than control. In contrast, Exg-D and CBHII displayed synergism during β-glucan degradation, displaying a DS of about 1.22. Thus, we propose that Exg-D should only be used synergistically with other CBHs to degrade mixed linked-β-(1,3)-(1,4)-glucan." @default.
• W3044221262 created "2020-07-29" @default.
• W3044221262 creator A5040319254 @default.
• W3044221262 creator A5049630676 @default.
• W3044221262 creator A5084351062 @default.
• W3044221262 date "2020-09-01" @default.
• W3044221262 modified "2023-09-27" @default.
• W3044221262 title "Delineating functional properties of a cello-oligosaccharide and β-glucan specific cellobiohydrolase (GH5_38): Its synergism with Cel6A and Cel7A for β-(1,3)-(1,4)-glucan degradation" @default.
• W3044221262 cites W1967229972 @default.
• W3044221262 cites W1969337506 @default.
• W3044221262 cites W1986343490 @default.
• W3044221262 cites W1988966516 @default.
• W3044221262 cites W1989089659 @default.
• W3044221262 cites W2009674410 @default.
• W3044221262 cites W2009690832 @default.
• W3044221262 cites W2014536330 @default.
• W3044221262 cites W2020443558 @default.
• W3044221262 cites W2034690533 @default.
• W3044221262 cites W2053359950 @default.
• W3044221262 cites W2063737089 @default.
• W3044221262 cites W2072799506 @default.
• W3044221262 cites W2100837269 @default.
• W3044221262 cites W2103990390 @default.
• W3044221262 cites W2104852630 @default.
• W3044221262 cites W2120548956 @default.
• W3044221262 cites W2120733511 @default.
• W3044221262 cites W2121306887 @default.
• W3044221262 cites W2141203896 @default.
• W3044221262 cites W2141860357 @default.
• W3044221262 cites W2144228064 @default.
• W3044221262 cites W2340811384 @default.
• W3044221262 cites W2464631400 @default.
• W3044221262 cites W2944479921 @default.
• W3044221262 cites W4293247451 @default.
• W3044221262 doi "https://doi.org/10.1016/j.carres.2020.108081" @default.
• W3044221262 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32738516" @default.
• W3044221262 hasPublicationYear "2020" @default.
• W3044221262 type Work @default.
• W3044221262 sameAs 3044221262 @default.
• W3044221262 citedByCount "13" @default.
• W3044221262 countsByYear W30442212622021 @default.
• W3044221262 countsByYear W30442212622022 @default.
• W3044221262 countsByYear W30442212622023 @default.
• W3044221262 crossrefType "journal-article" @default.
• W3044221262 hasAuthorship W3044221262A5040319254 @default.
• W3044221262 hasAuthorship W3044221262A5049630676 @default.
• W3044221262 hasAuthorship W3044221262A5084351062 @default.
• W3044221262 hasConcept C141603559 @default.
• W3044221262 hasConcept C185592680 @default.
• W3044221262 hasConcept C196032511 @default.
• W3044221262 hasConcept C2776123893 @default.
• W3044221262 hasConcept C2777726330 @default.
• W3044221262 hasConcept C2779251873 @default.
• W3044221262 hasConcept C2780412358 @default.
• W3044221262 hasConcept C43617362 @default.
• W3044221262 hasConcept C55493867 @default.
• W3044221262 hasConcept C94412978 @default.
• W3044221262 hasConceptScore W3044221262C141603559 @default.
• W3044221262 hasConceptScore W3044221262C185592680 @default.
• W3044221262 hasConceptScore W3044221262C196032511 @default.
• W3044221262 hasConceptScore W3044221262C2776123893 @default.
• W3044221262 hasConceptScore W3044221262C2777726330 @default.
• W3044221262 hasConceptScore W3044221262C2779251873 @default.
• W3044221262 hasConceptScore W3044221262C2780412358 @default.
• W3044221262 hasConceptScore W3044221262C43617362 @default.
• W3044221262 hasConceptScore W3044221262C55493867 @default.
• W3044221262 hasConceptScore W3044221262C94412978 @default.
• W3044221262 hasFunder F4320314988 @default.
• W3044221262 hasFunder F4320320671 @default.
• W3044221262 hasFunder F4320320682 @default.
• W3044221262 hasFunder F4320320683 @default.
• W3044221262 hasFunder F4320320691 @default.
• W3044221262 hasFunder F4320326716 @default.
• W3044221262 hasLocation W30442212621 @default.
• W3044221262 hasOpenAccess W3044221262 @default.
• W3044221262 hasPrimaryLocation W30442212621 @default.
• W3044221262 hasRelatedWork W1994894136 @default.
• W3044221262 hasRelatedWork W2017651669 @default.
• W3044221262 hasRelatedWork W2042871475 @default.
• W3044221262 hasRelatedWork W2053751536 @default.
• W3044221262 hasRelatedWork W2061896302 @default.
• W3044221262 hasRelatedWork W2072331814 @default.
• W3044221262 hasRelatedWork W2098308085 @default.
• W3044221262 hasRelatedWork W2134972352 @default.
• W3044221262 hasRelatedWork W2388038485 @default.
• W3044221262 hasRelatedWork W4296620618 @default.
• W3044221262 hasVolume "495" @default.
• W3044221262 isParatext "false" @default.
• W3044221262 isRetracted "false" @default.
• W3044221262 magId "3044221262" @default.
• W3044221262 workType "article" @default.