FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W30443154> ?p ?o ?g. }

• W30443154 abstract "Biomass derived products will play a significant role in the development towards a sustainable society. One major issue in the production of bio-based chemicals, using microorganisms as cell-factories, is the high and therefore inhibitory concentration of acetic acid, released during pretreatment of lignocellulose raw material. Acetic acid toxicity in Saccharomyces cerevisiae has been explained by reduced intracellular pH [1], accumulation of the acetate anion [2], and signaling effects triggering cell death [3]. The undissociated form of acetic acid enters the cell by passive diffusion over the lipid bilayer, although recent publications also suggest the involvement of Fps1p, aquaglyceroporin channel in the acetate uptake [4]. The aim of the present study is to investigate the potential relationship between plasma membrane lipid composition and acetic acid resistance, in the two yeasts S. cerevisiae and Zygosaccharomyces bailii. Z. bailii is a common food spoilage yeast, typically isolated from acetic acid rich environments such as vinegar or pickles and is used in this study as a model for acetic acid resistance. Previous publications demonstrated that Z. bailii unlike S. cerevisiae exhibit retained intracellular pH [5] and plasma membrane integrity [6] upon exposure to acetic acid. Detailed lipidomic profiling of glycerophospholipids, sphingolipids and sterols using multiple-reaction-monitoring mass spectrometry (MRM-MS) has been performed with total lipids extracts from S. cerevisiae and Z. bailii cultured in the absence and presence of acetic acid. Lipidome analysis pointed out sphingolipids as a key mediator in acetic acid resistance with strong increase in both S. cerevisiae and Z. bailii, when cultured with acetic acid. First, the basal levels of complex sphingolipids were 13 times higher in Z. bailii, possibly explaining the higher acetic acid resistance of this yeast species in comparison to S. cerevisiae. Moreover Z. bailii showed an astounding ability to remodel its membrane composition upon acetic acid stress, with strong increase in complex sphingolipids and a drastic reduction of glycerophospholipids. The next step of this study will be to use the lipid data to develop a strategy to engineer the lipid metabolism of S. cerevisiae towards increased acetic acid resistance. [1] Casal et al. FEMS Microbiol Rev (2008) 32(6): 974; [2] Russel. J. Appl Bacteriol (1992) 73: 363, [3] Ludovico et al. Microbiology (2001) 147:2409, [4] Mollapour et al. Mol Cell Biol (2007) 27(18):6446, [5] Arneborg et al. Archives of Microbiology (2000) 174(1-2):125, [6] Prudencio et al. Cytometry (1998) 31(4):307" @default.
• W30443154 created "2016-06-24" @default.
• W30443154 creator A5001617316 @default.
• W30443154 creator A5034405101 @default.
• W30443154 creator A5069748190 @default.
• W30443154 creator A5070933720 @default.
• W30443154 creator A5075140394 @default.
• W30443154 date "2013-01-01" @default.
• W30443154 modified "2023-09-27" @default.
• W30443154 title "Sphingolipids: A potential key to acetic acid resistance in Zygosaccharomyces bailii" @default.
• W30443154 hasPublicationYear "2013" @default.
• W30443154 type Work @default.
• W30443154 sameAs 30443154 @default.
• W30443154 citedByCount "0" @default.
• W30443154 crossrefType "journal-article" @default.
• W30443154 hasAuthorship W30443154A5001617316 @default.
• W30443154 hasAuthorship W30443154A5034405101 @default.
• W30443154 hasAuthorship W30443154A5069748190 @default.
• W30443154 hasAuthorship W30443154A5070933720 @default.
• W30443154 hasAuthorship W30443154A5075140394 @default.
• W30443154 hasConcept C185592680 @default.
• W30443154 hasConcept C2776673659 @default.
• W30443154 hasConcept C2779222958 @default.
• W30443154 hasConcept C2780241833 @default.
• W30443154 hasConcept C55493867 @default.
• W30443154 hasConcept C58017582 @default.
• W30443154 hasConcept C61705674 @default.
• W30443154 hasConcept C86803240 @default.
• W30443154 hasConceptScore W30443154C185592680 @default.
• W30443154 hasConceptScore W30443154C2776673659 @default.
• W30443154 hasConceptScore W30443154C2779222958 @default.
• W30443154 hasConceptScore W30443154C2780241833 @default.
• W30443154 hasConceptScore W30443154C55493867 @default.
• W30443154 hasConceptScore W30443154C58017582 @default.
• W30443154 hasConceptScore W30443154C61705674 @default.
• W30443154 hasConceptScore W30443154C86803240 @default.
• W30443154 hasLocation W304431541 @default.
• W30443154 hasOpenAccess W30443154 @default.
• W30443154 hasPrimaryLocation W304431541 @default.
• W30443154 hasRelatedWork W1011039798 @default.
• W30443154 hasRelatedWork W117178064 @default.
• W30443154 hasRelatedWork W1740382656 @default.
• W30443154 hasRelatedWork W1982143394 @default.
• W30443154 hasRelatedWork W1997281662 @default.
• W30443154 hasRelatedWork W2024151434 @default.
• W30443154 hasRelatedWork W2033093530 @default.
• W30443154 hasRelatedWork W2095202805 @default.
• W30443154 hasRelatedWork W2098609634 @default.
• W30443154 hasRelatedWork W2115422703 @default.
• W30443154 hasRelatedWork W2146412303 @default.
• W30443154 hasRelatedWork W2336231424 @default.
• W30443154 hasRelatedWork W2501748230 @default.
• W30443154 hasRelatedWork W2616472592 @default.
• W30443154 hasRelatedWork W2626607916 @default.
• W30443154 hasRelatedWork W27387244 @default.
• W30443154 hasRelatedWork W27637728 @default.
• W30443154 hasRelatedWork W2765155618 @default.
• W30443154 hasRelatedWork W2999703745 @default.
• W30443154 hasRelatedWork W592546175 @default.
• W30443154 isParatext "false" @default.
• W30443154 isRetracted "false" @default.
• W30443154 magId "30443154" @default.
• W30443154 workType "article" @default.