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- W3044339056 abstract "Abstract The Cre- loxP recombination system is a powerful tool for genetic manipulation. However, there are widely recognized limitations with chemically inducible Cre- loxP systems, and the UV and blue-light induced systems have phototoxicity and minimal capacity for deep tissue penetration. Here, we develop a far-red light-induced split Cre- loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL). The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination. Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver. Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery. Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems." @default.
- W3044339056 created "2020-07-29" @default.
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- W3044339056 date "2020-07-24" @default.
- W3044339056 modified "2023-10-07" @default.
- W3044339056 title "A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice" @default.
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- W3044339056 doi "https://doi.org/10.1038/s41467-020-17530-9" @default.
- W3044339056 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7381682" @default.
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