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- W3044406368 abstract "Viral-induced exacerbation of asthma remains a major cause of hospitalization and mortality. New human-relevant models of the airways are urgently needed to understand how respiratory infections may trigger asthma attacks and to advance treatment development. Here, we describe a new human-relevant model of rhinovirus-induced asthma exacerbation that recapitulates viral infection of asthmatic airway epithelium and neutrophil transepithelial migration, and enables evaluation of immunomodulatory therapy. Specifically, a microengineered model of fully differentiated human mucociliary airway epithelium was stimulated with IL-13 to induce a T-helper cell type 2 asthmatic phenotype and infected with live human rhinovirus 16 (HRV16) to reproduce key features of viral-induced asthma exacerbation. We observed that the infection with HRV16 replicated key hallmarks of the cytopathology and inflammatory responses observed in human airways. Generation of a T-helper cell type 2 microenvironment through exogenous IL-13 stimulation induced features of asthmatic airways, including goblet cell hyperplasia, reduction of cilia beating frequency, and endothelial activation, but did not alter rhinovirus infectivity or replication. High-resolution kinetic analysis of secreted inflammatory markers revealed that IL-13 treatment altered IL-6, IFN-λ1, and CXCL10 secretion in response to HRV16. Neutrophil transepithelial migration was greatest when viral infection was combined with IL-13 treatment, whereas treatment with MK-7123, a CXCR2 antagonist, reduced neutrophil diapedesis in all conditions. In conclusion, our microengineered Airway Lung-Chip provides a novel human-relevant platform for exploring the complex mechanisms underlying viral-induced asthma exacerbation. Our data suggest that IL-13 may impair the hosts’ ability to mount an appropriate and coordinated immune response to rhinovirus infection. We also show that the Airway Lung-Chip can be used to assess the efficacy of modulators of the immune response." @default.
- W3044406368 created "2020-07-29" @default.
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- W3044406368 date "2020-11-01" @default.
- W3044406368 modified "2023-10-10" @default.
- W3044406368 title "A Microengineered Airway Lung Chip Models Key Features of Viral-induced Exacerbation of Asthma" @default.
- W3044406368 cites W1868988913 @default.
- W3044406368 cites W1928785183 @default.
- W3044406368 cites W1948268265 @default.
- W3044406368 cites W1963632824 @default.
- W3044406368 cites W1964779747 @default.
- W3044406368 cites W1966647482 @default.
- W3044406368 cites W1980361506 @default.
- W3044406368 cites W1981720530 @default.
- W3044406368 cites W1982146873 @default.
- W3044406368 cites W1982839782 @default.
- W3044406368 cites W1984804064 @default.
- W3044406368 cites W1990470477 @default.
- W3044406368 cites W1992622607 @default.
- W3044406368 cites W1997794457 @default.
- W3044406368 cites W1998057092 @default.
- W3044406368 cites W2004765105 @default.
- W3044406368 cites W2005503891 @default.
- W3044406368 cites W2007736853 @default.
- W3044406368 cites W2015891257 @default.
- W3044406368 cites W2021351287 @default.
- W3044406368 cites W2023685287 @default.
- W3044406368 cites W2032227357 @default.
- W3044406368 cites W2034904367 @default.
- W3044406368 cites W2043357132 @default.
- W3044406368 cites W2043990114 @default.
- W3044406368 cites W2045650143 @default.
- W3044406368 cites W2053838728 @default.
- W3044406368 cites W2058783927 @default.
- W3044406368 cites W2061435251 @default.
- W3044406368 cites W2065366950 @default.
- W3044406368 cites W2080011794 @default.
- W3044406368 cites W2082484800 @default.
- W3044406368 cites W2086051107 @default.
- W3044406368 cites W2086550564 @default.
- W3044406368 cites W2086611150 @default.
- W3044406368 cites W2088293221 @default.
- W3044406368 cites W2091174106 @default.
- W3044406368 cites W2091467012 @default.
- W3044406368 cites W2097147259 @default.
- W3044406368 cites W2098333708 @default.
- W3044406368 cites W2099642924 @default.
- W3044406368 cites W2108528458 @default.
- W3044406368 cites W2110981320 @default.
- W3044406368 cites W2111704171 @default.
- W3044406368 cites W2112433605 @default.
- W3044406368 cites W2113226741 @default.
- W3044406368 cites W2113663343 @default.
- W3044406368 cites W2114203473 @default.
- W3044406368 cites W2114770406 @default.
- W3044406368 cites W2115693059 @default.
- W3044406368 cites W2117559606 @default.
- W3044406368 cites W2118364170 @default.
- W3044406368 cites W2134554530 @default.
- W3044406368 cites W2137313291 @default.
- W3044406368 cites W2140775892 @default.
- W3044406368 cites W2144165078 @default.
- W3044406368 cites W2147885743 @default.
- W3044406368 cites W2150300306 @default.
- W3044406368 cites W2150925133 @default.
- W3044406368 cites W2156958204 @default.
- W3044406368 cites W2158687521 @default.
- W3044406368 cites W2165397327 @default.
- W3044406368 cites W2177181431 @default.
- W3044406368 cites W2226413734 @default.
- W3044406368 cites W2256963343 @default.
- W3044406368 cites W2540468843 @default.
- W3044406368 cites W2600838862 @default.
- W3044406368 cites W2610113239 @default.
- W3044406368 cites W2623740869 @default.
- W3044406368 cites W2732245134 @default.
- W3044406368 cites W2743335049 @default.
- W3044406368 cites W2754644807 @default.
- W3044406368 cites W2756707886 @default.
- W3044406368 cites W2781782949 @default.
- W3044406368 cites W2791377680 @default.
- W3044406368 cites W2801344019 @default.
- W3044406368 cites W2803753995 @default.
- W3044406368 cites W2946833774 @default.
- W3044406368 cites W2963583927 @default.
- W3044406368 cites W2974149214 @default.