FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044442901> ?p ?o ?g. }

• W3044442901 abstract "This project sought to establish how lanthanide metals (Ln) bind to cell membranes, whether they are transported into cells and how they are distributed in vivo. Europium (Eu), chosen as a representative of the Ln series, as the chloride salt, formed stable aggregates (microprecipitates) in solution at neutral pH, even in phosphate- and bicarbonate-free conditions. The uptake of EuCI3 by intestinal brush-border membrane vesicles (BBMV) was difficult to determine because the aggregates interfered with the BBMV uptake assay and the concentration of available Eu3+ was not known. When tested as Eu-citrate, Eu-nitrilotriacetate and other complexes (ratio of Eu:ligand, 1:2) of intermediate stability constants (log KS of about 7 - 12) Eu was available for uptake by BBMV in phosphate- and bicarbonate-free conditions. Complexes with lower log KS did not keep Eu in solution whilst those of higher stability did not donate it to BBMV. The relationship, expressed as a reverse-sigmoidal curve, between log Ks and uptake of Eu by BBMV was consistent for most complexes with the exception of Eu-catechol complex. Eu bound to BBMV at two sites, as judged by displacement with the powerful ligand diethylenetriaminepentaacetic acid (DTPA). Binding to the DTP A-sensitive (membrane exterior) site predominated at 20°C. Uptake by the DTPA-resistant site was markedly enhanced at 37°C, suggesting a strong association with the membrane. Only small amounts (< 10 %) of bound Eu were released into the vesicle interior, confirming the poor membrane penetration of Ln ions. Thus Ln binding may be responsible for cytoprotective effects and changes in BBMV shape which were observed with Ln. Eu precipitation from most complexes was promoted in solutions containing phosphate and bicarbonate. However, the Eu-DTPA complex was stable in physiological solutions. Pre-formed Eu-albumin, despite a relatively low log KS (6. 4), was more stable in physiological solutions than complexes of log KS of 7 - 12. Eu precipitation in physiological solutions could also be prevented by increasing ligand:Eu ratio. When injected intravenously into rats, EuCI3 was sequestered by the liver and spleen. Eu in the liver was present in parenchymal and non-parenchymal cells. Eu injected as the Eu-albumin and Eu-DTPA complexes was rapidly excreted in the urine; a small but significant amount of Eu was retained in the kidney. EuCl3 did not effect Ca2+ uptake by BBMV and HoCI3 increased the calcium content of isolated hepatocytes, suggesting that Ln might not inhibitors of Ca2+ transport in all biological systems. These results question the validity of ubiquitously using Ln as analogues of calcium." @default.
• W3044442901 created "2020-07-29" @default.
• W3044442901 creator A5055176294 @default.
• W3044442901 date "1992-01-01" @default.
• W3044442901 modified "2023-09-27" @default.
• W3044442901 title "Cellular Uptake and Distribution of Lanthanides." @default.
• W3044442901 cites W1023756099 @default.
• W3044442901 cites W1044951679 @default.
• W3044442901 cites W105091413 @default.
• W3044442901 cites W1501295033 @default.
• W3044442901 cites W1525820657 @default.
• W3044442901 cites W1544673104 @default.
• W3044442901 cites W1564829038 @default.
• W3044442901 cites W1579144739 @default.
• W3044442901 cites W1616220687 @default.
• W3044442901 cites W1625881288 @default.
• W3044442901 cites W1775749144 @default.
• W3044442901 cites W1786092303 @default.
• W3044442901 cites W1962765392 @default.
• W3044442901 cites W1970587738 @default.
• W3044442901 cites W1970901862 @default.
• W3044442901 cites W1972487977 @default.
• W3044442901 cites W1976133945 @default.
• W3044442901 cites W1976984864 @default.
• W3044442901 cites W1988105325 @default.
• W3044442901 cites W1988388977 @default.
• W3044442901 cites W1989626681 @default.
• W3044442901 cites W1992479089 @default.
• W3044442901 cites W1993005923 @default.
• W3044442901 cites W1994853113 @default.
• W3044442901 cites W1995922817 @default.
• W3044442901 cites W1995976462 @default.
• W3044442901 cites W1997759067 @default.
• W3044442901 cites W1998007530 @default.
• W3044442901 cites W1998373022 @default.
• W3044442901 cites W2000958871 @default.
• W3044442901 cites W2004207541 @default.
• W3044442901 cites W2004912987 @default.
• W3044442901 cites W2007559239 @default.
• W3044442901 cites W2008051913 @default.
• W3044442901 cites W2012345358 @default.
• W3044442901 cites W2013866418 @default.
• W3044442901 cites W2015859497 @default.
• W3044442901 cites W2022407465 @default.
• W3044442901 cites W2023148968 @default.
• W3044442901 cites W2026150382 @default.
• W3044442901 cites W2027089014 @default.
• W3044442901 cites W2029785826 @default.
• W3044442901 cites W2032458072 @default.
• W3044442901 cites W2034231595 @default.
• W3044442901 cites W2034878493 @default.
• W3044442901 cites W2035564590 @default.
• W3044442901 cites W2037761808 @default.
• W3044442901 cites W2042375106 @default.
• W3044442901 cites W2049514922 @default.
• W3044442901 cites W2057123878 @default.
• W3044442901 cites W2061728105 @default.
• W3044442901 cites W2064240780 @default.
• W3044442901 cites W20659841 @default.
• W3044442901 cites W2067814812 @default.
• W3044442901 cites W2072224568 @default.
• W3044442901 cites W2072721486 @default.
• W3044442901 cites W2073219495 @default.
• W3044442901 cites W2077422189 @default.
• W3044442901 cites W2077586572 @default.
• W3044442901 cites W2079054020 @default.
• W3044442901 cites W2080580789 @default.
• W3044442901 cites W2086198136 @default.
• W3044442901 cites W2091144616 @default.
• W3044442901 cites W2091656567 @default.
• W3044442901 cites W2110408216 @default.
• W3044442901 cites W2113943014 @default.
• W3044442901 cites W2134027848 @default.
• W3044442901 cites W2136330395 @default.
• W3044442901 cites W2136891864 @default.
• W3044442901 cites W2144136652 @default.
• W3044442901 cites W2152054410 @default.
• W3044442901 cites W2155230665 @default.
• W3044442901 cites W2158065200 @default.
• W3044442901 cites W2167590372 @default.
• W3044442901 cites W2174751052 @default.
• W3044442901 cites W2176061808 @default.
• W3044442901 cites W2184972586 @default.
• W3044442901 cites W2235348649 @default.
• W3044442901 cites W2265650765 @default.
• W3044442901 cites W2286462737 @default.
• W3044442901 cites W2319902937 @default.
• W3044442901 cites W2329802702 @default.
• W3044442901 cites W2408852316 @default.
• W3044442901 cites W2409073701 @default.
• W3044442901 cites W2440591304 @default.
• W3044442901 cites W2489993894 @default.
• W3044442901 cites W2509560316 @default.
• W3044442901 cites W3166029991 @default.
• W3044442901 cites W572553075 @default.
• W3044442901 cites W591832344 @default.
• W3044442901 cites W604312725 @default.
• W3044442901 cites W2027162582 @default.
• W3044442901 cites W3111355423 @default.
• W3044442901 hasPublicationYear "1992" @default.

• next