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- W3044505114 abstract "Abstract The fate of extracellular antibiotic resistance genes (eARGs) in waste activated sludge during anaerobic digestion (AD) remained unclear. The current study investigated the changes in seven eARGs (sulI, sulII, tet(A), tet(O), tet(X), blaTEM, and blaSHV) and intI1 during sludge AD at 35 °C and 55 °C. First, the extracellular DNA (eDNA) extraction method from sludge was optimized by adding sodium dodecyl sulfonate, and the eDNA recovery nearly doubled. Second, analysis via qPCR showed that eARGs ranged from 1.5% to 85.1% of the total ARGs, stressing the importance of eARGs in sludge. Besides, the abundances of all detected eARGs decreased following AD, where removal rates ranged from 22.8% to 93.9% at 35 °C and 52.7% to 96.6% at 55 °C. Further analysis showed that the removal rates of eARGs were negatively correlated with their initial abundances (P" @default.
- W3044505114 created "2020-07-29" @default.
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- W3044505114 date "2020-10-01" @default.
- W3044505114 modified "2023-09-24" @default.
- W3044505114 title "Anaerobic digestion reduces extracellular antibiotic resistance genes in waste activated sludge: The effects of temperature and degradation mechanisms" @default.
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- W3044505114 doi "https://doi.org/10.1016/j.envint.2020.105980" @default.
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