Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044506520> ?p ?o ?g. }
- W3044506520 endingPage "101654" @default.
- W3044506520 startingPage "101654" @default.
- W3044506520 abstract "In recent years, the gasotransmitter hydrogen sulphide (H2S), produced by the transsulphuration pathway, has been recognized as a biological mediator playing an important role under normal conditions and in various pathologies in both eukaryotes and prokaryotes. The transsulphuration pathway (TSP) includes the conversion of homocysteine to cysteine following the breakdown of methionine. In Drosophila melanogaster and other eukaryotes, H2S is produced by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulphurtransferase (MST). In the experiments performed in this study, we were able to explore the CRISPR/Cas9 technique to obtain single and double deletions in homozygotes of these three major genes responsible for H2S production in Drosophila melanogaster. In most cases, the deletion of one studied gene does not result in the compensatory induction of two other genes responsible for H2S production. Transcriptomic studies demonstrated that the deletions of the above CBS and CSE genes alter genome expression to different degrees, with a more pronounced effect being exerted by deletion of the CBS gene. Furthermore, the double deletion of both CBS and CSE resulted in a cumulative effect on transcription in the resulting strains. Overall, we found that the obtained deletions affect numerous genes involved in various biological pathways. Specifically, genes involved in the oxidative reduction process, stress-response genes, housekeeping genes, and genes participating in olfactory and reproduction are among the most strongly affected. Furthermore, characteristic differences in the response to the deletions of the studied genes are apparently organ-specific and have clear-cut sex-specific characteristics. Single and double deletions of the three genes responsible for the production of H2S helped to elucidate new aspects of the biological significance of this vital physiological mediator." @default.
- W3044506520 created "2020-07-29" @default.
- W3044506520 creator A5011805562 @default.
- W3044506520 creator A5016059874 @default.
- W3044506520 creator A5018408278 @default.
- W3044506520 creator A5023848026 @default.
- W3044506520 creator A5031996878 @default.
- W3044506520 creator A5036451566 @default.
- W3044506520 creator A5052916599 @default.
- W3044506520 creator A5056684612 @default.
- W3044506520 creator A5071575806 @default.
- W3044506520 creator A5074698747 @default.
- W3044506520 date "2020-09-01" @default.
- W3044506520 modified "2023-10-11" @default.
- W3044506520 title "Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster" @default.
- W3044506520 cites W1079142787 @default.
- W3044506520 cites W1548082920 @default.
- W3044506520 cites W1636270625 @default.
- W3044506520 cites W1855102004 @default.
- W3044506520 cites W1952752962 @default.
- W3044506520 cites W1963674655 @default.
- W3044506520 cites W1965123365 @default.
- W3044506520 cites W1967138582 @default.
- W3044506520 cites W1969256525 @default.
- W3044506520 cites W1969322923 @default.
- W3044506520 cites W1971439989 @default.
- W3044506520 cites W1977409932 @default.
- W3044506520 cites W1980014735 @default.
- W3044506520 cites W1982206418 @default.
- W3044506520 cites W1983058328 @default.
- W3044506520 cites W1983321171 @default.
- W3044506520 cites W1984206366 @default.
- W3044506520 cites W1984537876 @default.
- W3044506520 cites W1988097700 @default.
- W3044506520 cites W1992691152 @default.
- W3044506520 cites W1994251118 @default.
- W3044506520 cites W2001671099 @default.
- W3044506520 cites W2002216056 @default.
- W3044506520 cites W2003261639 @default.
- W3044506520 cites W2005493046 @default.
- W3044506520 cites W2007064404 @default.
- W3044506520 cites W2007419421 @default.
- W3044506520 cites W2009836431 @default.
- W3044506520 cites W2011653432 @default.
- W3044506520 cites W2012164150 @default.
- W3044506520 cites W2017744076 @default.
- W3044506520 cites W2021215174 @default.
- W3044506520 cites W2022031165 @default.
- W3044506520 cites W2023985961 @default.
- W3044506520 cites W2028644023 @default.
- W3044506520 cites W2030116088 @default.
- W3044506520 cites W2030135028 @default.
- W3044506520 cites W2033323717 @default.
- W3044506520 cites W2045787491 @default.
- W3044506520 cites W2049065271 @default.
- W3044506520 cites W2049229433 @default.
- W3044506520 cites W2055539787 @default.
- W3044506520 cites W2056558785 @default.
- W3044506520 cites W2057128621 @default.
- W3044506520 cites W2068308584 @default.
- W3044506520 cites W2070478726 @default.
- W3044506520 cites W2071210519 @default.
- W3044506520 cites W2071674582 @default.
- W3044506520 cites W2073632680 @default.
- W3044506520 cites W2074418236 @default.
- W3044506520 cites W2074840190 @default.
- W3044506520 cites W2082752342 @default.
- W3044506520 cites W2087795198 @default.
- W3044506520 cites W2090478056 @default.
- W3044506520 cites W2098086227 @default.
- W3044506520 cites W2112079415 @default.
- W3044506520 cites W2113526393 @default.
- W3044506520 cites W2127121072 @default.
- W3044506520 cites W2130906350 @default.
- W3044506520 cites W2130923760 @default.
- W3044506520 cites W2131123393 @default.
- W3044506520 cites W2135140367 @default.
- W3044506520 cites W2137961859 @default.
- W3044506520 cites W2141261198 @default.
- W3044506520 cites W2143224843 @default.
- W3044506520 cites W2147372470 @default.
- W3044506520 cites W2156667412 @default.
- W3044506520 cites W2172161196 @default.
- W3044506520 cites W2225379276 @default.
- W3044506520 cites W2244217889 @default.
- W3044506520 cites W2315657766 @default.
- W3044506520 cites W2346687813 @default.
- W3044506520 cites W2411356319 @default.
- W3044506520 cites W2461013690 @default.
- W3044506520 cites W2552043470 @default.
- W3044506520 cites W2614590942 @default.
- W3044506520 cites W2743408232 @default.
- W3044506520 cites W2749718141 @default.
- W3044506520 cites W2787873962 @default.
- W3044506520 cites W2791913728 @default.
- W3044506520 cites W2792937385 @default.
- W3044506520 cites W2808850074 @default.
- W3044506520 cites W2898204371 @default.