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- W3044672302 abstract "The xantha phenotype of barley is observable as a yellow plant and caused by an inability to correctly synthesize chlorophyll. Chlorophyll is a tetrapyrrolic pigment containing magnesium, and is integral for photosynthesis in plants. The phenotype can be caused by a number of mutations, and through methods like linkage mapping and sequencing it is possible to find the causative genotype. In this project xantha mutants xan-m and xan-j were investigated. The xan-m mutants were thought to possess mutations in the uncategorized gene HORVU3Hr1G050010 and xan-j a mutation in the chlorophyll synthase gene. The candidate gene of xan-m mutants is very large, and so only its transcribed mRNA was investigated through mRNA-selective RT-PCR and sequencing of the gene in eight fragments. A previously known mutation in the candidate gene of mutant xan-m.53 was in this project confirmed through sequencing. However, no other sequenced xan-m mutants possessed mutations in the candidate gene.This suggests that the candidate gene mutation is not the one causing the xantha phenotype. xan-j investigation through western blotting was during the project unsuccessful, due to ordered antibodies not being of sufficient concentration. (Less)" @default.
- W3044672302 created "2020-07-29" @default.
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- W3044672302 date "2020-01-01" @default.
- W3044672302 modified "2023-09-26" @default.
- W3044672302 title "Analysis of chlorophyll synthesis mutants xantha-m and xantha-j of Hordeum vulgare L." @default.
- W3044672302 hasPublicationYear "2020" @default.
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