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• W3044783947 abstract "Abstract Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766." @default.
• W3044783947 created "2020-07-29" @default.
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• W3044783947 date "2020-07-23" @default.
• W3044783947 modified "2023-09-26" @default.
• W3044783947 title "Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants" @default.
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• W3044783947 doi "https://doi.org/10.1093/infdis/jiaa448" @default.
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