Matches in SemOpenAlex for { <https://semopenalex.org/work/W3044804562> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W3044804562 endingPage "462" @default.
- W3044804562 startingPage "458" @default.
- W3044804562 abstract "To investigate the effects of mannose, the major component of Lycium barbarum polysaccharide, and its potential target metabolite, inositol, on mouse islet β-TC6 cells.Different concentrations(0, 4. 6875, 9. 375, 18. 75, 37. 5, 75 and 150 μg/mL) of mannose or inositol were used to intervene β-TC6 cells for 24 hours, and the proliferation activity of cells was determined by CCK-8 method. Enzyme-linked immunosorbent assay was used to detect insulin secretion after the intervention of the β-TC6 cells from different concentration of the mannose or inositol(0, 18. 75, 75 and 150 μg/mL) combining with glucose stimulation(20 mmol/L) for 60 minutes. Pioglitazone(3. 92 mg/L) was set up as positive group, and after intervention of the mannose or inositol(0, 9. 375, 18. 75, 75 and 150 μg/mL) for 24 h, the expression levels of insulin, glucose kinase(GK), glucose transporter 4(GLUT4) and glycogen synthase(GS) mRNA were detected by real-time quantitative PCR.Compared with the control group, mannose and inositol promoted the proliferation of β-TC6 cells in a concentration-dependent manner(18. 75-150 μg/mL)(P<0. 05). Although the inositol solution of 4. 6875 μg/mL and 9. 375 μg/mL had a tendency to promote cell proliferation, there was no statistical difference(P>0. 05). After stimulation with 20 mmol/L glucose combining with different intervention concentrations(18. 75, 75 and 150 μg/mL) of mannose or inositol, no significant difference was observed in the insulin secretion of each group(P>0. 05) comparing with the control group. RT-qPCR result showed that 150 μg/mL mannose increased the expression level of GLUT4(P<0. 01) and the expression levels of GK and GLUT4 genes in the 75 μg/mL inositol group were significantly increased(P<0. 01). The expression level of GLUT4 was improved only when the concentration was decreased to 18. 75 μg/mL in inositol group(P<0. 01).Mannose and inositol can improve the expression of GLUT4 mRNA, which may help to increase glucose uptake by peripheral cells. In addition, inositol can improve insulin sensitivity and glucose metabolism by increasing the expression level of GK mRNA." @default.
- W3044804562 created "2020-07-29" @default.
- W3044804562 creator A5018851857 @default.
- W3044804562 creator A5031555538 @default.
- W3044804562 creator A5049460049 @default.
- W3044804562 creator A5050775888 @default.
- W3044804562 creator A5056001730 @default.
- W3044804562 creator A5084980661 @default.
- W3044804562 date "2020-05-01" @default.
- W3044804562 modified "2023-09-25" @default.
- W3044804562 title "[Effects of the mannose, the major component of Lycium barbarum polysaccharides, and its potential target metabolite inositol on mouse β-TC6 cells]." @default.
- W3044804562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32693897" @default.
- W3044804562 hasPublicationYear "2020" @default.
- W3044804562 type Work @default.
- W3044804562 sameAs 3044804562 @default.
- W3044804562 citedByCount "0" @default.
- W3044804562 crossrefType "journal-article" @default.
- W3044804562 hasAuthorship W3044804562A5018851857 @default.
- W3044804562 hasAuthorship W3044804562A5031555538 @default.
- W3044804562 hasAuthorship W3044804562A5049460049 @default.
- W3044804562 hasAuthorship W3044804562A5050775888 @default.
- W3044804562 hasAuthorship W3044804562A5056001730 @default.
- W3044804562 hasAuthorship W3044804562A5084980661 @default.
- W3044804562 hasConcept C126322002 @default.
- W3044804562 hasConcept C134018914 @default.
- W3044804562 hasConcept C161573976 @default.
- W3044804562 hasConcept C170493617 @default.
- W3044804562 hasConcept C185592680 @default.
- W3044804562 hasConcept C2775887612 @default.
- W3044804562 hasConcept C2776188179 @default.
- W3044804562 hasConcept C2777427919 @default.
- W3044804562 hasConcept C2777477808 @default.
- W3044804562 hasConcept C2779306644 @default.
- W3044804562 hasConcept C55493867 @default.
- W3044804562 hasConcept C71924100 @default.
- W3044804562 hasConcept C86803240 @default.
- W3044804562 hasConceptScore W3044804562C126322002 @default.
- W3044804562 hasConceptScore W3044804562C134018914 @default.
- W3044804562 hasConceptScore W3044804562C161573976 @default.
- W3044804562 hasConceptScore W3044804562C170493617 @default.
- W3044804562 hasConceptScore W3044804562C185592680 @default.
- W3044804562 hasConceptScore W3044804562C2775887612 @default.
- W3044804562 hasConceptScore W3044804562C2776188179 @default.
- W3044804562 hasConceptScore W3044804562C2777427919 @default.
- W3044804562 hasConceptScore W3044804562C2777477808 @default.
- W3044804562 hasConceptScore W3044804562C2779306644 @default.
- W3044804562 hasConceptScore W3044804562C55493867 @default.
- W3044804562 hasConceptScore W3044804562C71924100 @default.
- W3044804562 hasConceptScore W3044804562C86803240 @default.
- W3044804562 hasIssue "3" @default.
- W3044804562 hasLocation W30448045621 @default.
- W3044804562 hasOpenAccess W3044804562 @default.
- W3044804562 hasPrimaryLocation W30448045621 @default.
- W3044804562 hasRelatedWork W1964898152 @default.
- W3044804562 hasRelatedWork W1990439561 @default.
- W3044804562 hasRelatedWork W2079678199 @default.
- W3044804562 hasRelatedWork W2087143164 @default.
- W3044804562 hasRelatedWork W2168667092 @default.
- W3044804562 hasRelatedWork W2356701840 @default.
- W3044804562 hasRelatedWork W2364471633 @default.
- W3044804562 hasRelatedWork W280628162 @default.
- W3044804562 hasRelatedWork W2901878533 @default.
- W3044804562 hasRelatedWork W3044804562 @default.
- W3044804562 hasVolume "49" @default.
- W3044804562 isParatext "false" @default.
- W3044804562 isRetracted "false" @default.
- W3044804562 magId "3044804562" @default.
- W3044804562 workType "article" @default.