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- W3045037117 abstract "Abstract Background: In absence of effective vaccines, infection prevention and control of SARS-CoV-2 through diagnostic testing and quarantine is critical. Early detection and differential diagnosis of respiratory infections increases the chances for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the current standard for molecular diagnosis with high sensitivity. However, the highest specificity confirmation target ORF1ab gene is considered to be less sensitive than other targets in clinical application. In addition, a large amount of recent evidence indicates that the initial missed diagnosis of asymptomatic patients with SARS-CoV-2 and discharged patients with “re-examination positive” may be due to low viral load, and the ability of rapid mutation of SARS-CoV-2 also increases the rate of false negative results. Moreover, the current used mixed sample nucleic acid detection is helpful to seek out the early community transmission of SARS-CoV-2, but the detection kit needs ultra-high detection sensitivity. Methods: From January to March 2020, 10 confirmed specimens of 2019-nCoV were recruited from three 2019-nCOV nucleic acid testing center. Five different amplification kits with three different primers and probes sources were selected. RT-PCR and continuous amplification of nasopharyngeal and oropharyngeal swabs and environmental specimens were performed to validate the sensitivity of the commercially available Nucleic acid test kits. Results: The results showed that ORF1ab gene can still be reported as positive at 1:10 dilution and the N gene even at 1:40 dilution with kit-1 through the verification of multiple positive samples, While other kits have less sensitive. The results in the suspicious range of weakly positive nasopharyngeal and oropharyngeal swabs and environmental specimens could be reported as positive after another re-amplification. Conclusions: Through evaluate the sensitivity of different nucleic acid detection kits, this study provide direct evidence for the selection of kits for mixed sample detection or make recommendations for the selection of validation kit, which is of great significance for the prevention and control of the current epidemic and the discharge criteria of low viral load patients." @default.
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- W3045037117 date "2020-07-07" @default.
- W3045037117 modified "2023-09-28" @default.
- W3045037117 title "Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative" @default.
- W3045037117 doi "https://doi.org/10.21203/rs.3.rs-40414/v1" @default.
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