FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3045464598> ?p ?o ?g. }

• W3045464598 abstract "Small conductance, calcium-activated (SK3) potassium channels control the intrinsic excitability of dopaminergic neurons (DN) in the midbrain and modulate their susceptibility to toxic insults during development.We evaluated the age-dependency of the neuroprotective effect of an SK3 agonist, 1-Ethyl-1,3-dihydro-2H-benzimidazol-2-one (1-EBIO), on Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) excitotoxicity to DN in ventral mesencephalon (VM) organotypic cultures.Most tyrosine hydroxylase (TH)+ neurons were also SK3+; SK3+/TH- cells (DN+) were common at each developmental stage but more prominently at day in vitro (DIV) 8. Young DN+ neurons were small bipolar and fusiform, whereas mature ones were large and multipolar. Exposure of organotypic cultures to AMPA (100 μm, 16 h) had no effect on the survival of DN+ at DIV 8, but caused significant toxicity at DIV 15 (n = 15, p = 0.005) and DIV 22 (n = 15, p<0.001). These results indicate that susceptibility of DN to AMPA excitotoxicity is developmental stage-dependent in embryonic VM organotypic cultures. Immature DN+ (small, bipolar) were increased after AMPA (100 μm, 16 h) at DIV 8, at the expense of the number of differentiated (large, multipolar) DN+ (p = 0.039). This effect was larger at DIV 15 (p<<<0.0001) and at DIV 22 (p<<<0.0001). At DIV 8, 30 μM 1-EBIO resulted in a large increase in DN+. At DIV 15, AMPA toxicity was prevented by exposure to 30 μM, but not 100 μM 1-EBIO. At DIV 22, excitotoxicity was unaffected by 30 μM 1-EBIO, and partially reduced by 100 μM 1-EBIO.The effects of the SK3 channel agonist 1-EBIO on the survival of SK3-expressing dopaminergic neurons were concentration-dependent and influenced by neuronal developmental stage." @default.
• W3045464598 created "2020-07-29" @default.
• W3045464598 creator A5002923739 @default.
• W3045464598 creator A5016688715 @default.
• W3045464598 creator A5023333046 @default.
• W3045464598 creator A5052669707 @default.
• W3045464598 creator A5057374532 @default.
• W3045464598 creator A5068989151 @default.
• W3045464598 creator A5086741096 @default.
• W3045464598 creator A5064150561 @default.
• W3045464598 date "2020-07-23" @default.
• W3045464598 modified "2023-09-24" @default.
• W3045464598 title "Age-dependent neuroprotective effect of an SK3 channel agonist on excitotoxity to dopaminergic neurons in organotypic culture" @default.
• W3045464598 cites W118456516 @default.
• W3045464598 cites W1533604136 @default.
• W3045464598 cites W1542145035 @default.
• W3045464598 cites W1823302046 @default.
• W3045464598 cites W1971104810 @default.
• W3045464598 cites W1973874549 @default.
• W3045464598 cites W1974737922 @default.
• W3045464598 cites W1982253693 @default.
• W3045464598 cites W1988100039 @default.
• W3045464598 cites W1997857782 @default.
• W3045464598 cites W1999281581 @default.
• W3045464598 cites W2000072607 @default.
• W3045464598 cites W2001043844 @default.
• W3045464598 cites W2001551525 @default.
• W3045464598 cites W2012070790 @default.
• W3045464598 cites W2022062323 @default.
• W3045464598 cites W2027899918 @default.
• W3045464598 cites W2035034074 @default.
• W3045464598 cites W2035511726 @default.
• W3045464598 cites W2039578967 @default.
• W3045464598 cites W2040416693 @default.
• W3045464598 cites W2047523290 @default.
• W3045464598 cites W2047968402 @default.
• W3045464598 cites W2049007928 @default.
• W3045464598 cites W2049944911 @default.
• W3045464598 cites W2067510074 @default.
• W3045464598 cites W2082917853 @default.
• W3045464598 cites W2084142451 @default.
• W3045464598 cites W2092934163 @default.
• W3045464598 cites W2093852589 @default.
• W3045464598 cites W2095212000 @default.
• W3045464598 cites W2100068597 @default.
• W3045464598 cites W2109104280 @default.
• W3045464598 cites W2116739536 @default.
• W3045464598 cites W2122289819 @default.
• W3045464598 cites W2124346415 @default.
• W3045464598 cites W2129114637 @default.
• W3045464598 cites W2135687823 @default.
• W3045464598 cites W2139826597 @default.
• W3045464598 cites W2145387500 @default.
• W3045464598 cites W2158125090 @default.
• W3045464598 cites W2274641679 @default.
• W3045464598 cites W2546820741 @default.
• W3045464598 cites W2566718637 @default.
• W3045464598 cites W2736942460 @default.
• W3045464598 cites W2896883303 @default.
• W3045464598 cites W2903796247 @default.
• W3045464598 cites W2948094573 @default.
• W3045464598 cites W2069559939 @default.
• W3045464598 doi "https://doi.org/10.1371/journal.pone.0223633" @default.
• W3045464598 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7377472" @default.
• W3045464598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32701951" @default.
• W3045464598 hasPublicationYear "2020" @default.
• W3045464598 type Work @default.
• W3045464598 sameAs 3045464598 @default.
• W3045464598 citedByCount "0" @default.
• W3045464598 crossrefType "journal-article" @default.
• W3045464598 hasAuthorship W3045464598A5002923739 @default.
• W3045464598 hasAuthorship W3045464598A5016688715 @default.
• W3045464598 hasAuthorship W3045464598A5023333046 @default.
• W3045464598 hasAuthorship W3045464598A5052669707 @default.
• W3045464598 hasAuthorship W3045464598A5057374532 @default.
• W3045464598 hasAuthorship W3045464598A5064150561 @default.
• W3045464598 hasAuthorship W3045464598A5068989151 @default.
• W3045464598 hasAuthorship W3045464598A5086741096 @default.
• W3045464598 hasBestOaLocation W30454645981 @default.
• W3045464598 hasConcept C126322002 @default.
• W3045464598 hasConcept C134018914 @default.
• W3045464598 hasConcept C137183658 @default.
• W3045464598 hasConcept C160268369 @default.
• W3045464598 hasConcept C169760540 @default.
• W3045464598 hasConcept C170493617 @default.
• W3045464598 hasConcept C185592680 @default.
• W3045464598 hasConcept C25498285 @default.
• W3045464598 hasConcept C2778674702 @default.
• W3045464598 hasConcept C2778938600 @default.
• W3045464598 hasConcept C2781012912 @default.
• W3045464598 hasConcept C513476851 @default.
• W3045464598 hasConcept C61174792 @default.
• W3045464598 hasConcept C71924100 @default.
• W3045464598 hasConcept C86803240 @default.
• W3045464598 hasConcept C98274493 @default.
• W3045464598 hasConceptScore W3045464598C126322002 @default.
• W3045464598 hasConceptScore W3045464598C134018914 @default.
• W3045464598 hasConceptScore W3045464598C137183658 @default.
• W3045464598 hasConceptScore W3045464598C160268369 @default.
• W3045464598 hasConceptScore W3045464598C169760540 @default.

• next