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- W3045572242 abstract "ABSTRACT Our understanding of how the obligate intracellular bacterium Chlamydia trachomatis reprograms the cell biology of host cells in the upper genital tract is largely based on observations made in cell culture with transformed epithelial cell lines. Here we describe a primary spherical organoid system derived from endometrial tissue to recapitulate epithelial cell diversity, polarity, and ensuing responses to Chlamydia infection. Using high-resolution and time-lapse microscopy, we catalogue the infection process in organoids from invasion to egress, including the reorganization of the cytoskeleton and positioning of intracellular organelles. We show this model is amenable to screening C. trachomatis mutants for defects in the fusion of pathogenic vacuoles, the recruitment of intracellular organelles, and inhibition of cell death. Moreover, we reconstructed a primary immune cell response by co-culturing infected organoids with neutrophils, and determined that the effector TepP limits the recruitment of neutrophils to infected organoids. Collectively, our model details a system to study the cell biology of Chlamydia infections in three dimensional structures that better reflect the diversity of cell types and polarity encountered by Chlamydia upon infection of their animal hosts. Summary statement 3D endometrial organoids to model Chlamydia infection and the role of secreted virulence factors in reprogramming host epithelial cells and immune cell recruitment" @default.
- W3045572242 created "2020-08-03" @default.
- W3045572242 creator A5020319196 @default.
- W3045572242 creator A5021498852 @default.
- W3045572242 date "2020-07-29" @default.
- W3045572242 modified "2023-09-27" @default.
- W3045572242 title "An endometrial organoid model ofChlamydia-epithelial and immune cell interactions" @default.
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- W3045572242 doi "https://doi.org/10.1101/2020.07.29.226969" @default.
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