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- W3045767937 abstract "目的为探究脂多糖对O1、O78血清型禽致病性大肠杆菌（Avian pathogenic Escherichia coli，APEC）致病作用的影响。 方法选取负责脂质A生物合成相关基因lpxL和lpxM，利用λ噬菌体的Red同源重组系统分别构建APEC E516（O1血清型）和APEC E522（O78血清型）缺失株E516ΔlpxL、E516ΔlpxM、E516ΔlpxLΔlpxM、E522ΔlpxL、E522ΔlpxM和E522ΔlpxLΔlpxM，并通过体内外试验对其生物学特性及致病性进行研究。 结果各菌株生长速度基本一致。E516ΔlpxL、E516ΔlpxLΔlpxM、E522ΔlpxM和E522ΔlpxLΔlpxM的抗血清补体杀菌能力和抗鸡巨噬细胞HD-11吞噬能力较野生株显著下降，而缺失株E516ΔlpxM、E522ΔlpxL与野生株相比无明显差异；半数致死剂量测定结果显示，除E516ΔlpxM、E522ΔlpxL外，各缺失株毒力降低1000倍左右；SPF鸡体内动态分布试验结果显示，各缺失株在鸡体内定殖能力较野生株显著下降，但回补株的毒力未能恢复至野生株水平。 结论lpxL和lpxM基因与O1血清型APEC E516株和O78血清型APEC E522株的毒力有关，但是lpxL和lpxM基因对E516和E522菌株毒力的影响存在差异。" @default.
- W3045767937 created "2020-08-03" @default.
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- W3045767937 date "2020-07-04" @default.
- W3045767937 modified "2023-09-25" @default.
- W3045767937 title "lpxL 和 lpxM 基因对O1、O78血清型禽致病性大肠杆菌毒力的影响" @default.
- W3045767937 doi "https://doi.org/10.13343/j.cnki.wsxb.20200047" @default.
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