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- W3045817313 abstract "Thermophilic microbes are an attractive bioproduction platform due to their inherently lower contamination risk and their ability to perform thermostable enzymatic processes which may be required for biomass processing and other industrial applications. The engineering of microbes for industrial scale processes requires a suite of genetic engineering tools to optimize existing biological systems as well as to design and incorporate new metabolic pathways within strains. Yet, such tools are often lacking and/or inadequate for novel microbes, especially thermophiles. This chapter focuses on genetic tool development and engineering strategies, in addition to challenges, for thermophilic microbes. We provide detailed instructions and techniques for tool development for an anaerobic thermophile, Caldanaerobacter subterraneus subsp. tengcongensis, including culturing, plasmid construction, transformation, and selection. This establishes a foundation for advanced genetic tool development necessary for the metabolic engineering of this microbe and potentially other thermophilic organisms." @default.
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- W3045817313 date "2020-01-01" @default.
- W3045817313 modified "2023-09-25" @default.
- W3045817313 title "Gene Editing Technologies for Biofuel Production in Thermophilic Microbes" @default.
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- W3045817313 doi "https://doi.org/10.1007/978-1-0716-0195-2_12" @default.
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