Matches in SemOpenAlex for { <https://semopenalex.org/work/W3045900210> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3045900210 endingPage "19714" @default.
- W3045900210 startingPage "19702" @default.
- W3045900210 abstract "This study developed a new rapid transcription activator-like effector nuclease (TALEN) preparation protocol by thoroughly redesigning the widely used Golden Gate TALEN and TAL Effector Kit 2.0. The new protocol can be used to prepare any custom 18-bp binding TALENs in just one day (about 12 h), more rapidly than CRISPR. This protocol used a set of linear monomers, a final TALE-FokI backbone plasmid, and a pipeline to assemble the ready-to-use TALEN expression plasmid, which were all newly developed for this study. The set of linear monomers can be easily produced and reproduced by high-fidelity polymerase chain reaction (PCR) amplification in a 96-well plate using a pair of universal primers. Most important of all, our rapid TALEN construction pipeline can easily obtain many positive colonies with high efficiency (over 80%). By preparing five pairs of TALENs targeting five NF-κB genes (RELA, RELB, CREL,NFKB1, and NFKB2) and editing these genes in different cell lines (293T, HepG2, and PANC1), this study demonstrated that the new protocol has high efficiency, reproducibility, reliability, and applicability. Moreover, this study showed that the fabricated TALEN has much higher editing efficiency than CRISPR. Finally, this study developed a dual-tagging system for simultaneously tagging target proteins and successfully edited cells with a streptavidin-binding peptide (SBP) or AviTag via homology-directed repair, which could have wide applications in protein (antigen) preparation, immunoprecipitation, and a transcription factor chromatin immunoprecipitation assay." @default.
- W3045900210 created "2020-08-03" @default.
- W3045900210 creator A5027065447 @default.
- W3045900210 creator A5085960035 @default.
- W3045900210 creator A5090613548 @default.
- W3045900210 date "2020-07-29" @default.
- W3045900210 modified "2023-09-28" @default.
- W3045900210 title "One-Day TALEN Assembly Protocol and a Dual-Tagging System for Genome Editing" @default.
- W3045900210 cites W1976312969 @default.
- W3045900210 cites W1978129709 @default.
- W3045900210 cites W1991389689 @default.
- W3045900210 cites W1997250478 @default.
- W3045900210 cites W2002767268 @default.
- W3045900210 cites W2018509153 @default.
- W3045900210 cites W2050757906 @default.
- W3045900210 cites W2065471902 @default.
- W3045900210 cites W2073377349 @default.
- W3045900210 cites W2073873559 @default.
- W3045900210 cites W2076840637 @default.
- W3045900210 cites W2077659966 @default.
- W3045900210 cites W2082131511 @default.
- W3045900210 cites W2087264052 @default.
- W3045900210 cites W2088816616 @default.
- W3045900210 cites W2101027021 @default.
- W3045900210 cites W2102484627 @default.
- W3045900210 cites W2106859063 @default.
- W3045900210 cites W2112006537 @default.
- W3045900210 cites W2115268444 @default.
- W3045900210 cites W2121670270 @default.
- W3045900210 cites W2130931930 @default.
- W3045900210 cites W2137209165 @default.
- W3045900210 cites W2165447216 @default.
- W3045900210 cites W2168245648 @default.
- W3045900210 cites W2170551524 @default.
- W3045900210 cites W2461122889 @default.
- W3045900210 cites W2583070206 @default.
- W3045900210 cites W2597423497 @default.
- W3045900210 cites W2616802735 @default.
- W3045900210 cites W2727344742 @default.
- W3045900210 cites W2918057622 @default.
- W3045900210 doi "https://doi.org/10.1021/acsomega.0c02396" @default.
- W3045900210 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7424704" @default.
- W3045900210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32803065" @default.
- W3045900210 hasPublicationYear "2020" @default.
- W3045900210 type Work @default.
- W3045900210 sameAs 3045900210 @default.
- W3045900210 citedByCount "8" @default.
- W3045900210 countsByYear W30459002102021 @default.
- W3045900210 countsByYear W30459002102022 @default.
- W3045900210 countsByYear W30459002102023 @default.
- W3045900210 crossrefType "journal-article" @default.
- W3045900210 hasAuthorship W3045900210A5027065447 @default.
- W3045900210 hasAuthorship W3045900210A5085960035 @default.
- W3045900210 hasAuthorship W3045900210A5090613548 @default.
- W3045900210 hasBestOaLocation W30459002101 @default.
- W3045900210 hasConcept C104317684 @default.
- W3045900210 hasConcept C144501496 @default.
- W3045900210 hasConcept C31909778 @default.
- W3045900210 hasConcept C41008148 @default.
- W3045900210 hasConcept C51785407 @default.
- W3045900210 hasConcept C54355233 @default.
- W3045900210 hasConcept C70721500 @default.
- W3045900210 hasConcept C86803240 @default.
- W3045900210 hasConcept C95444343 @default.
- W3045900210 hasConcept C98108389 @default.
- W3045900210 hasConceptScore W3045900210C104317684 @default.
- W3045900210 hasConceptScore W3045900210C144501496 @default.
- W3045900210 hasConceptScore W3045900210C31909778 @default.
- W3045900210 hasConceptScore W3045900210C41008148 @default.
- W3045900210 hasConceptScore W3045900210C51785407 @default.
- W3045900210 hasConceptScore W3045900210C54355233 @default.
- W3045900210 hasConceptScore W3045900210C70721500 @default.
- W3045900210 hasConceptScore W3045900210C86803240 @default.
- W3045900210 hasConceptScore W3045900210C95444343 @default.
- W3045900210 hasConceptScore W3045900210C98108389 @default.
- W3045900210 hasFunder F4320321001 @default.
- W3045900210 hasIssue "31" @default.
- W3045900210 hasLocation W30459002101 @default.
- W3045900210 hasLocation W30459002102 @default.
- W3045900210 hasLocation W30459002103 @default.
- W3045900210 hasLocation W30459002104 @default.
- W3045900210 hasOpenAccess W3045900210 @default.
- W3045900210 hasPrimaryLocation W30459002101 @default.
- W3045900210 hasRelatedWork W1905889610 @default.
- W3045900210 hasRelatedWork W2078846065 @default.
- W3045900210 hasRelatedWork W2125469076 @default.
- W3045900210 hasRelatedWork W2391066184 @default.
- W3045900210 hasRelatedWork W2478165495 @default.
- W3045900210 hasRelatedWork W2519421663 @default.
- W3045900210 hasRelatedWork W3208910293 @default.
- W3045900210 hasRelatedWork W4319265337 @default.
- W3045900210 hasRelatedWork W4320919304 @default.
- W3045900210 hasRelatedWork W4366975683 @default.
- W3045900210 hasVolume "5" @default.
- W3045900210 isParatext "false" @default.
- W3045900210 isRetracted "false" @default.
- W3045900210 magId "3045900210" @default.
- W3045900210 workType "article" @default.