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• W3046056234 abstract "The objective of this study is to visualize the ability of cell proliferation based on molecular beacons (MB). Two types of MB to detect messenger RNA (mRNA) were used. One is a Ki67 MB of a target for cell proliferation ability. The other one is a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) MB as a control of stable fluorescence in cells. To enhance the MB internalization into cells, the MB were incorporated into cationized gelatin nanospheres (cGNS). There was no difference in the physicochemical properties and the cell internalization between the cGNSKi67 MB and cGNSGAP MB. When basic fibroblast growth factor (bFGF) was added to KUM6 cells of a mouse bone marrow-derived mesenchymal stem cell line, the expression of Ki67 and the cell proliferation increased with the bFGF concentration. After the incubation for the cell internalization of cGNS incorporating MB (cGNSMB), the cells were further incubated for 24 h with or without different concentrations of bFGF. The fluorescence of cGNSKi67 MB significantly increased with the increase of bFGF concentration, whereas that of cGNSGAP MB was constant, irrespective of the bFGF concentration. A time-lapse imaging assay revealed a fast enhancement of cGNSKi67 MB fluorescence after the bFGF addition compared with no bFGF addition. On the other hand, for cGNSGAP MB, a constant fluorescence was observed even at any time point after the bFGF addition. It is concluded that the cGNSMB system is promising for the chronological visualization of proliferation ability in living cells." @default.
• W3046056234 created "2020-08-03" @default.
• W3046056234 creator A5012775133 @default.
• W3046056234 creator A5055033211 @default.
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• W3046056234 date "2020-09-18" @default.
• W3046056234 modified "2023-10-16" @default.
• W3046056234 title "Molecular Beacon Imaging to Visualize Ki67 mRNA for Cell Proliferation Ability" @default.
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• W3046056234 doi "https://doi.org/10.1089/ten.tea.2020.0127" @default.
• W3046056234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32723028" @default.
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