FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3046082854> ?p ?o ?g. }

• W3046082854 endingPage "382" @default.
• W3046082854 startingPage "372" @default.
• W3046082854 abstract "Abstract Purpose With the increase of especially hospital-acquired infections, timely and accurate diagnosis of bacterial infections is crucial for effective patient care. Molecular imaging has the potential for specific and sensitive detection of infections. Siderophores are iron-specific chelators recognized by specific bacterial transporters, representing one of few fundamental differences between bacterial and mammalian cells. Replacing iron by gallium-68 without loss of bioactivity is possible allowing molecular imaging by positron emission tomography (PET). Here, we report on the preclinical evaluation of the clinically used siderophore, desferrioxamine-B (Desferal®, DFO-B), radiolabelled with 68 Ga for imaging of bacterial infections. Methods In vitro characterization of [ 68 Ga]Ga-DFO-B included partition coefficient, protein binding and stability determination. Specific uptake of [ 68 Ga]Ga-DFO-B was tested in vitro in different microbial cultures. In vivo biodistribution was studied in healthy mice and dosimetric estimation for human setting performed. PET/CT imaging was carried out in animal infection models, representing the most common pathogens. Results DFO-B was labelled with 68 Ga with high radiochemical purity and displayed hydrophilic properties, low protein binding and high stability in human serum and PBS. The high in vitro uptake of [ 68 Ga]Ga-DFO-B in selected strains of Pseudomonas aeruginosa , Staphylococcus aureus and Streptococcus agalactiae could be blocked with an excess of iron-DFO-B. [ 68 Ga]Ga-DFO-B showed rapid renal excretion and minimal retention in blood and other organs in healthy mice. Estimated human absorbed dose was 0.02 mSv/MBq. PET/CT images of animal infection models displayed high and specific accumulation of [ 68 Ga]Ga-DFO-B in both P. aeruginosa and S. aureus infections with excellent image contrast. No uptake was found in sterile inflammation, heat-inactivated P. aeruginosa or S. aureus and Escherichia coli lacking DFO-B transporters. Conclusion DFO-B can be easily radiolabelled with 68 Ga and displayed suitable in vitro characteristics and excellent pharmacokinetics in mice. The high and specific uptake of [ 68 Ga]Ga-DFO-B by P. aeruginosa and S. aureus was confirmed both in vitro and in vivo, proving the potential of [ 68 Ga]Ga-DFO-B for specific imaging of bacterial infections. As DFO-B is used in clinic for many years and the estimated radiation dose is lower than for other 68 Ga-labelled radiopharmaceuticals, we believe that [ 68 Ga]Ga-DFO-B has a great potential for clinical translation." @default.
• W3046082854 created "2020-08-03" @default.
• W3046082854 creator A5004358070 @default.
• W3046082854 creator A5006169657 @default.
• W3046082854 creator A5016827125 @default.
• W3046082854 creator A5017213061 @default.
• W3046082854 creator A5026503568 @default.
• W3046082854 creator A5030725736 @default.
• W3046082854 creator A5067471151 @default.
• W3046082854 creator A5082845375 @default.
• W3046082854 creator A5082889749 @default.
• W3046082854 creator A5085313408 @default.
• W3046082854 creator A5088212408 @default.
• W3046082854 date "2020-07-30" @default.
• W3046082854 modified "2023-10-17" @default.
• W3046082854 title "68Ga-labelled desferrioxamine-B for bacterial infection imaging" @default.
• W3046082854 cites W1993548586 @default.
• W3046082854 cites W1995094885 @default.
• W3046082854 cites W2044493144 @default.
• W3046082854 cites W2044678473 @default.
• W3046082854 cites W2060976020 @default.
• W3046082854 cites W2075326272 @default.
• W3046082854 cites W2079608198 @default.
• W3046082854 cites W2099143902 @default.
• W3046082854 cites W2099164168 @default.
• W3046082854 cites W2102816693 @default.
• W3046082854 cites W2104523238 @default.
• W3046082854 cites W2112309782 @default.
• W3046082854 cites W2115269770 @default.
• W3046082854 cites W2121041639 @default.
• W3046082854 cites W2144929147 @default.
• W3046082854 cites W2157355263 @default.
• W3046082854 cites W2161377113 @default.
• W3046082854 cites W2167835598 @default.
• W3046082854 cites W2312698043 @default.
• W3046082854 cites W2347102183 @default.
• W3046082854 cites W2356983239 @default.
• W3046082854 cites W2530663934 @default.
• W3046082854 cites W2563679383 @default.
• W3046082854 cites W2583405382 @default.
• W3046082854 cites W2584360689 @default.
• W3046082854 cites W2621078430 @default.
• W3046082854 cites W2739735646 @default.
• W3046082854 cites W2750282724 @default.
• W3046082854 cites W2783494381 @default.
• W3046082854 cites W2810361189 @default.
• W3046082854 cites W2896710856 @default.
• W3046082854 cites W2901222459 @default.
• W3046082854 cites W2907121739 @default.
• W3046082854 cites W2934656438 @default.
• W3046082854 cites W2948068236 @default.
• W3046082854 cites W2971878379 @default.
• W3046082854 cites W2985445683 @default.
• W3046082854 cites W3003244256 @default.
• W3046082854 cites W3005684149 @default.
• W3046082854 cites W3191084815 @default.
• W3046082854 doi "https://doi.org/10.1007/s00259-020-04948-y" @default.
• W3046082854 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7835195" @default.
• W3046082854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32734456" @default.
• W3046082854 hasPublicationYear "2020" @default.
• W3046082854 type Work @default.
• W3046082854 sameAs 3046082854 @default.
• W3046082854 citedByCount "43" @default.
• W3046082854 countsByYear W30460828542020 @default.
• W3046082854 countsByYear W30460828542021 @default.
• W3046082854 countsByYear W30460828542022 @default.
• W3046082854 countsByYear W30460828542023 @default.
• W3046082854 crossrefType "journal-article" @default.
• W3046082854 hasAuthorship W3046082854A5004358070 @default.
• W3046082854 hasAuthorship W3046082854A5006169657 @default.
• W3046082854 hasAuthorship W3046082854A5016827125 @default.
• W3046082854 hasAuthorship W3046082854A5017213061 @default.
• W3046082854 hasAuthorship W3046082854A5026503568 @default.
• W3046082854 hasAuthorship W3046082854A5030725736 @default.
• W3046082854 hasAuthorship W3046082854A5067471151 @default.
• W3046082854 hasAuthorship W3046082854A5082845375 @default.
• W3046082854 hasAuthorship W3046082854A5082889749 @default.
• W3046082854 hasAuthorship W3046082854A5085313408 @default.
• W3046082854 hasAuthorship W3046082854A5088212408 @default.
• W3046082854 hasBestOaLocation W30460828541 @default.
• W3046082854 hasConcept C150903083 @default.
• W3046082854 hasConcept C185592680 @default.
• W3046082854 hasConcept C202751555 @default.
• W3046082854 hasConcept C207001950 @default.
• W3046082854 hasConcept C2775842073 @default.
• W3046082854 hasConcept C2777807558 @default.
• W3046082854 hasConcept C2777860557 @default.
• W3046082854 hasConcept C2778276568 @default.
• W3046082854 hasConcept C2779489039 @default.
• W3046082854 hasConcept C2989005 @default.
• W3046082854 hasConcept C523546767 @default.
• W3046082854 hasConcept C54355233 @default.
• W3046082854 hasConcept C55493867 @default.
• W3046082854 hasConcept C71924100 @default.
• W3046082854 hasConcept C86803240 @default.
• W3046082854 hasConcept C89423630 @default.
• W3046082854 hasConceptScore W3046082854C150903083 @default.
• W3046082854 hasConceptScore W3046082854C185592680 @default.

• next