Matches in SemOpenAlex for { <https://semopenalex.org/work/W3046121337> ?p ?o ?g. }
- W3046121337 abstract "Abstract Background Furfural and 5-hydroxymethyl furfural (5-HMF) are key furan inhibitors that are generated due to breakdown of lignocellulosic sugars at high temperature and acidic treatment conditions. Both furfural and 5-HMF act in a synergistic manner to inhibit microbial metabolism and resistance to both is a desirable characteristic for efficient conversion of lignocellulosic carbon to ethanol. Genetic manipulations targeted toward increasing cellular NADPH pools have successfully imparted tolerance against furfural and 5-HMF. In present study, deletion of pgi gene as a strategy to augment carbon flow through pentose phosphate pathway (PPP) was studied in ethanologenic Escherichia coli strain SSK101 to impart tolerance towards either furfural or 5-HMFor both inhibitors together. Results A key gene of EMP pathway, pgi , was deleted in an ethanologenic E. coli strain SSK42 to yield strain SSK101. In presence of 1 g/L furfural in minimal AM1 media, the rate of biomass formation for strain SSK101 was up to 1.9-fold higher as compared to parent SSK42 strain, and it was able to clear furfural in half the time. Tolerance to inhibitor was associated with glucose as carbon source and not xylose, and the tolerance advantage of SSK101 was neutralized in LB media. Bioreactor studies were performed under binary stress of furfural and 5-HMF (1 g/L each) and different glucose concentrations in a glucose–xylose mixture with final sugar concentration of 5.5%, mimicking major components of dilute acid treated biomass hydrolysate. In the mixture having 6 g/L and 12 g/L glucose, SSK101 strain produced ~ 18 g/L and 20 g/L ethanol, respectively. Interestingly, the maximum ethanol productivity was better at lower glucose load with 0.46 g/(L.h) between 96 and 120 h, as compared to higher glucose load where it was 0.33 g/(L.h) between 144 and 168 h. Importantly, parent strain SSK42 did not exhibit significant metabolic activity under similar conditions of inhibitor load and sugar concentration. Conclusions E. coli strain SSK101 with pgi deletion had enhanced tolerance against both furfural and 5-HMF, which was associated with presence of glucose in media. Strain SSK101 also had improved fermentation characteristics under both hyperosmotic as well as binary stress of furfural and 5-HMF in media containing glucose–xylose mixture." @default.
- W3046121337 created "2020-08-03" @default.
- W3046121337 creator A5050359447 @default.
- W3046121337 creator A5052718025 @default.
- W3046121337 creator A5058911046 @default.
- W3046121337 creator A5066975867 @default.
- W3046121337 creator A5073667046 @default.
- W3046121337 creator A5077115805 @default.
- W3046121337 date "2020-07-28" @default.
- W3046121337 modified "2023-10-16" @default.
- W3046121337 title "Deletion of pgi gene in E. coli increases tolerance to furfural and 5-hydroxymethyl furfural in media containing glucose–xylose mixture" @default.
- W3046121337 cites W126741892 @default.
- W3046121337 cites W1567678295 @default.
- W3046121337 cites W1579875904 @default.
- W3046121337 cites W1901474357 @default.
- W3046121337 cites W1978153633 @default.
- W3046121337 cites W1984402343 @default.
- W3046121337 cites W1986082851 @default.
- W3046121337 cites W1997488092 @default.
- W3046121337 cites W2005291574 @default.
- W3046121337 cites W2009921752 @default.
- W3046121337 cites W2017030483 @default.
- W3046121337 cites W2023854629 @default.
- W3046121337 cites W2027966927 @default.
- W3046121337 cites W2038024290 @default.
- W3046121337 cites W2038176196 @default.
- W3046121337 cites W2039736177 @default.
- W3046121337 cites W2041048626 @default.
- W3046121337 cites W2053711823 @default.
- W3046121337 cites W2093057648 @default.
- W3046121337 cites W2109193964 @default.
- W3046121337 cites W2114967847 @default.
- W3046121337 cites W2116137883 @default.
- W3046121337 cites W2122293981 @default.
- W3046121337 cites W2125652910 @default.
- W3046121337 cites W2131985559 @default.
- W3046121337 cites W2134293661 @default.
- W3046121337 cites W2137100180 @default.
- W3046121337 cites W2140485535 @default.
- W3046121337 cites W2146599462 @default.
- W3046121337 cites W2148033653 @default.
- W3046121337 cites W2165126425 @default.
- W3046121337 cites W2553578439 @default.
- W3046121337 cites W2729776710 @default.
- W3046121337 cites W2909884327 @default.
- W3046121337 cites W3188915507 @default.
- W3046121337 doi "https://doi.org/10.1186/s12934-020-01414-0" @default.
- W3046121337 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7389444" @default.
- W3046121337 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32723338" @default.
- W3046121337 hasPublicationYear "2020" @default.
- W3046121337 type Work @default.
- W3046121337 sameAs 3046121337 @default.
- W3046121337 citedByCount "14" @default.
- W3046121337 countsByYear W30461213372021 @default.
- W3046121337 countsByYear W30461213372022 @default.
- W3046121337 countsByYear W30461213372023 @default.
- W3046121337 crossrefType "journal-article" @default.
- W3046121337 hasAuthorship W3046121337A5050359447 @default.
- W3046121337 hasAuthorship W3046121337A5052718025 @default.
- W3046121337 hasAuthorship W3046121337A5058911046 @default.
- W3046121337 hasAuthorship W3046121337A5066975867 @default.
- W3046121337 hasAuthorship W3046121337A5073667046 @default.
- W3046121337 hasAuthorship W3046121337A5077115805 @default.
- W3046121337 hasBestOaLocation W30461213371 @default.
- W3046121337 hasConcept C100544194 @default.
- W3046121337 hasConcept C161790260 @default.
- W3046121337 hasConcept C185592680 @default.
- W3046121337 hasConcept C2777108408 @default.
- W3046121337 hasConcept C2778234585 @default.
- W3046121337 hasConcept C2779719330 @default.
- W3046121337 hasConcept C2780294150 @default.
- W3046121337 hasConcept C28800991 @default.
- W3046121337 hasConcept C31903555 @default.
- W3046121337 hasConcept C55493867 @default.
- W3046121337 hasConcept C94412978 @default.
- W3046121337 hasConceptScore W3046121337C100544194 @default.
- W3046121337 hasConceptScore W3046121337C161790260 @default.
- W3046121337 hasConceptScore W3046121337C185592680 @default.
- W3046121337 hasConceptScore W3046121337C2777108408 @default.
- W3046121337 hasConceptScore W3046121337C2778234585 @default.
- W3046121337 hasConceptScore W3046121337C2779719330 @default.
- W3046121337 hasConceptScore W3046121337C2780294150 @default.
- W3046121337 hasConceptScore W3046121337C28800991 @default.
- W3046121337 hasConceptScore W3046121337C31903555 @default.
- W3046121337 hasConceptScore W3046121337C55493867 @default.
- W3046121337 hasConceptScore W3046121337C94412978 @default.
- W3046121337 hasFunder F4320320717 @default.
- W3046121337 hasIssue "1" @default.
- W3046121337 hasLocation W30461213371 @default.
- W3046121337 hasLocation W30461213372 @default.
- W3046121337 hasLocation W30461213373 @default.
- W3046121337 hasLocation W30461213374 @default.
- W3046121337 hasOpenAccess W3046121337 @default.
- W3046121337 hasPrimaryLocation W30461213371 @default.
- W3046121337 hasRelatedWork W1974540837 @default.
- W3046121337 hasRelatedWork W2036200277 @default.
- W3046121337 hasRelatedWork W2064671512 @default.
- W3046121337 hasRelatedWork W2094954630 @default.
- W3046121337 hasRelatedWork W2768169160 @default.
- W3046121337 hasRelatedWork W2896185329 @default.