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• W3046251505 endingPage "e0227529" @default.
• W3046251505 startingPage "e0227529" @default.
• W3046251505 abstract "The pretreatment of biomass remains a critical requirement for bio-renewable fuel production from lignocellulose. Although current processes primarily involve chemical and physical approaches, the biological breakdown of lignin using enzymes and microorganisms is quickly becoming an interesting eco-friendly alternative to classical processes. As a result, bioprospection of wild fungi from naturally occurring lignin-rich sources remains a suitable method to uncover and isolate new species exhibiting ligninolytic activity. In this study, wild species of white rot fungi were collected from Colombian forests based on their natural wood decay ability and high capacity to secrete oxidoreductases with high affinity for phenolic polymers such as lignin. Based on high activity obtained from solid-state fermentation using a lignocellulose source from oil palm as matrix, we describe the isolation and whole-genome sequencing of Dictyopanus pusillus, a wild basidiomycete fungus exhibiting ABTS oxidation as an indication of laccase activity. Functional characterization of a crude enzymatic extract identified laccase activity as the main enzymatic contributor to fungal extracts, an observation supported by the identification of 13 putative genes encoding for homologous laccases in the genome. To the best of our knowledge, this represents the first report of an enzymatic extract exhibiting laccase activity in the Dictyopanus genera, offering means to exploit this species and its enzymes for the delignification process of lignocellulosic by-products from oil palm." @default.
• W3046251505 created "2020-08-07" @default.
• W3046251505 creator A5001973072 @default.
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• W3046251505 date "2020-07-30" @default.
• W3046251505 modified "2023-10-18" @default.
• W3046251505 title "Genome sequencing and functional characterization of a Dictyopanus pusillus fungal enzymatic extract offers a promising alternative for lignocellulose pretreatment of oil palm residues" @default.
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• W3046251505 doi "https://doi.org/10.1371/journal.pone.0227529" @default.
• W3046251505 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7392265" @default.
• W3046251505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32730337" @default.
• W3046251505 hasPublicationYear "2020" @default.
• W3046251505 type Work @default.
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