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- W3046409348 abstract "目的 通过构建成纤维细胞生长因子-21基因（fibroblast growth factor 21，FGF-21）荧光素酶报告基因载体，观察has-miR-577和has-miR-583对FGF-21基因的靶向调控。方法 利用生物学信息网站对has-miR-577和has-miR-583靶向结合FGF-21 3′UTR区的位点进行分析，设计合成包含与has-miR-577和has-miR-583结合序列及其突变序列的FGF-21基因片段，构建野生型（psiCHECK2-FGF-21）和突变型（psiCHECK2-FGF-21-mut）FGF-21双荧光素酶报告基因载体。进行双酶切电泳和测序鉴定后，FGF-21野生型与突变型双荧光素酶报告基因载体分别+〔hsa-miR-577模拟物、hsa-miR-583模拟物和miR无义序列阴性对照 (miR negative control，miR-NC)〕转染293T 细胞，检测荧光素酶活性，观察has-miR-577和has-miR-583对FGF-21表达的影响。结果 双酶切电泳和测序结果显示，野生型（psiCHECK2-FGF-21）和突变型（psiCHECK2-FGF-21-mut）基因载体片段大小、序列结果与实验预期一致，载体构建成功。has-miR-577和has-miR-583可抑制野生型FGF-21载体的荧光表达(PFGF-21载体的荧光表达无抑制作用。结论 has-miR-577和has-miR-583可以靶向调控FGF-21的表达。" @default.
- W3046409348 created "2020-08-07" @default.
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- W3046409348 date "2016-11-21" @default.
- W3046409348 modified "2023-09-23" @default.
- W3046409348 title "双荧光素酶报告基因系统鉴定has-miR-577和has-miR-583对FGF-21基因的靶向调控" @default.
- W3046409348 hasPublicationYear "2016" @default.
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