FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3046752464> ?p ?o ?g. }

• W3046752464 endingPage "102014" @default.
• W3046752464 startingPage "102014" @default.
• W3046752464 abstract "Using synthetic biology, we can turn algae into bio-factories that produce high-value molecules (e.g. medicines or biofuels) or tackle global challenges (e.g. malnutrition and climate change). This realization has provoked rapid progress towards the creation of genetic tools for multiple algal species, notably Phaeodactylum tricornutum. The power of synthetic biology to generate more useful organisms depends on the ability to produce diverse DNA molecules and rapidly screen them for beneficial variants in chosen hosts. However, it is still relatively expensive to synthesize DNA, and delivering large DNA (>50 kbp) to eukaryotic cellular compartments remains challenging. In this study, we establish a robust system for building designer algal mitochondrial genomes ranging in sizes from approximately 60 to 95 kbp as a practical alternative to DNA synthesis. Our approach permits the inexpensive and rapid generation of mitochondrial derivatives designed for testing targeted DNA delivery. First, we cloned the mitochondrial genome of P. tricornutum into the eukaryotic host Saccharomyces cerevisiae using two different techniques: transformation-associated recombination; and PCR-based cloning. Next, we screened the cloned genomes by multiplex PCR, and transformed correct genomes into the prokaryotic host organism Escherichia coli. These genomes were again analyzed by multiplex PCR, followed by diagnostic digest and complete plasmid sequencing to evaluate the fidelity of each cloning method. Finally, we assessed the burden on eukaryotic and prokaryotic hosts to propagate the cloned genomes. We conclude that our system can reliably generate variants for genome-level engineering of algal mitochondria." @default.
• W3046752464 created "2020-08-07" @default.
• W3046752464 creator A5003586270 @default.
• W3046752464 creator A5004177913 @default.
• W3046752464 creator A5016108826 @default.
• W3046752464 creator A5044481199 @default.
• W3046752464 creator A5052179215 @default.
• W3046752464 creator A5058279665 @default.
• W3046752464 creator A5058546523 @default.
• W3046752464 creator A5070285273 @default.
• W3046752464 creator A5075632480 @default.
• W3046752464 creator A5082114171 @default.
• W3046752464 date "2020-09-01" @default.
• W3046752464 modified "2023-09-26" @default.
• W3046752464 title "Rapid method for generating designer algal mitochondrial genomes" @default.
• W3046752464 cites W1602683653 @default.
• W3046752464 cites W1963511036 @default.
• W3046752464 cites W1971196369 @default.
• W3046752464 cites W1975135854 @default.
• W3046752464 cites W1984248485 @default.
• W3046752464 cites W1988672718 @default.
• W3046752464 cites W1989191870 @default.
• W3046752464 cites W1990060260 @default.
• W3046752464 cites W2002602569 @default.
• W3046752464 cites W2005190180 @default.
• W3046752464 cites W2016417802 @default.
• W3046752464 cites W2019115334 @default.
• W3046752464 cites W2027921745 @default.
• W3046752464 cites W2034927153 @default.
• W3046752464 cites W2036577945 @default.
• W3046752464 cites W2037045595 @default.
• W3046752464 cites W2038226984 @default.
• W3046752464 cites W2040928146 @default.
• W3046752464 cites W2041250319 @default.
• W3046752464 cites W2071524074 @default.
• W3046752464 cites W2073052887 @default.
• W3046752464 cites W2082529446 @default.
• W3046752464 cites W2085980948 @default.
• W3046752464 cites W2142455927 @default.
• W3046752464 cites W2153108117 @default.
• W3046752464 cites W2161273903 @default.
• W3046752464 cites W2166907286 @default.
• W3046752464 cites W2219394610 @default.
• W3046752464 cites W2304674135 @default.
• W3046752464 cites W2401329037 @default.
• W3046752464 cites W2417180743 @default.
• W3046752464 cites W2514783055 @default.
• W3046752464 cites W2595907366 @default.
• W3046752464 cites W2613197076 @default.
• W3046752464 cites W2732481151 @default.
• W3046752464 cites W2782004254 @default.
• W3046752464 cites W2888288916 @default.
• W3046752464 cites W2944969418 @default.
• W3046752464 cites W2949719113 @default.
• W3046752464 cites W2978295770 @default.
• W3046752464 cites W2980929848 @default.
• W3046752464 cites W2982359977 @default.
• W3046752464 doi "https://doi.org/10.1016/j.algal.2020.102014" @default.
• W3046752464 hasPublicationYear "2020" @default.
• W3046752464 type Work @default.
• W3046752464 sameAs 3046752464 @default.
• W3046752464 citedByCount "14" @default.
• W3046752464 countsByYear W30467524642020 @default.
• W3046752464 countsByYear W30467524642021 @default.
• W3046752464 countsByYear W30467524642022 @default.
• W3046752464 countsByYear W30467524642023 @default.
• W3046752464 crossrefType "journal-article" @default.
• W3046752464 hasAuthorship W3046752464A5003586270 @default.
• W3046752464 hasAuthorship W3046752464A5004177913 @default.
• W3046752464 hasAuthorship W3046752464A5016108826 @default.
• W3046752464 hasAuthorship W3046752464A5044481199 @default.
• W3046752464 hasAuthorship W3046752464A5052179215 @default.
• W3046752464 hasAuthorship W3046752464A5058279665 @default.
• W3046752464 hasAuthorship W3046752464A5058546523 @default.
• W3046752464 hasAuthorship W3046752464A5070285273 @default.
• W3046752464 hasAuthorship W3046752464A5075632480 @default.
• W3046752464 hasAuthorship W3046752464A5082114171 @default.
• W3046752464 hasBestOaLocation W30467524642 @default.
• W3046752464 hasConcept C104317684 @default.
• W3046752464 hasConcept C121050878 @default.
• W3046752464 hasConcept C141231307 @default.
• W3046752464 hasConcept C144501496 @default.
• W3046752464 hasConcept C145290725 @default.
• W3046752464 hasConcept C18903297 @default.
• W3046752464 hasConcept C191908910 @default.
• W3046752464 hasConcept C199360897 @default.
• W3046752464 hasConcept C24586158 @default.
• W3046752464 hasConcept C2780655307 @default.
• W3046752464 hasConcept C41008148 @default.
• W3046752464 hasConcept C54355233 @default.
• W3046752464 hasConcept C559758991 @default.
• W3046752464 hasConcept C70721500 @default.
• W3046752464 hasConcept C86803240 @default.
• W3046752464 hasConceptScore W3046752464C104317684 @default.
• W3046752464 hasConceptScore W3046752464C121050878 @default.
• W3046752464 hasConceptScore W3046752464C141231307 @default.
• W3046752464 hasConceptScore W3046752464C144501496 @default.
• W3046752464 hasConceptScore W3046752464C145290725 @default.

• next