FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3046973944> ?p ?o ?g. }

• W3046973944 endingPage "1754" @default.
• W3046973944 startingPage "1744" @default.
• W3046973944 abstract "Abstract The ATP6V1G1 subunit (V1G1) of the vacuolar proton ATPase (V-ATPase) pump is crucial for glioma stem cells (GSC) maintenance and in vivo tumorigenicity. Moreover, V-ATPase reprograms the tumor microenvironment through acidification and release of extracellular vesicles (EV). Therefore, we investigated the role of V1G1 in GSC small EVs and their effects on primary brain cultures. To this end, small EVs were isolated from patients-derived GSCs grown as neurospheres (NS) with high (V1G1HIGH-NS) or low (V1G1LOW-NS) V1G1 expression and analyzed for V-ATPase subunits presence, miRNA contents, and cellular responses in recipient cultures. Our results show that NS-derived small EVs stimulate proliferation and motility of recipient cells, with small EV derived from V1G1HIGH-NS showing the most pronounced activity. This involved activation of ERK1/2 signaling, in a response reversed by V-ATPase inhibition in NS-producing small EV. The miRNA profile of V1G1HIGH-NS–derived small EVs differed significantly from that of V1G1LOW-NS, which included miRNAs predicted to target MAPK/ERK signaling. Mechanistically, forced expression of a MAPK-targeting pool of miRNAs in recipient cells suppressed MAPK/ERK pathway activation and blunted the prooncogenic effects of V1G1HIGH small EV. These findings propose that the GSC influences the brain milieu through a V1G1-coordinated EVs release of MAPK/ERK-targeting miRNAs. Interfering with V-ATPase activity could prevent ERK-dependent oncogenic reprogramming of the microenvironment, potentially hampering local GBM infiltration. Implications: Our data identify a novel molecular mechanism of gliomagenesis specific of the GBM stem cell niche, which coordinates a V-ATPase–dependent reprogramming of the brain microenvironment through the release of specialized EVs." @default.
• W3046973944 created "2020-08-10" @default.
• W3046973944 creator A5005608989 @default.
• W3046973944 creator A5007639428 @default.
• W3046973944 creator A5037434863 @default.
• W3046973944 creator A5038705314 @default.
• W3046973944 creator A5040774134 @default.
• W3046973944 creator A5067688250 @default.
• W3046973944 creator A5073583842 @default.
• W3046973944 creator A5082346201 @default.
• W3046973944 creator A5089929964 @default.
• W3046973944 date "2020-11-01" @default.
• W3046973944 modified "2023-10-16" @default.
• W3046973944 title "Interplay Between V-ATPase G1 and Small EV-miRNAs Modulates ERK1/2 Activation in GBM Stem Cells and Nonneoplastic Milieu" @default.
• W3046973944 cites W1520761649 @default.
• W3046973944 cites W1571111796 @default.
• W3046973944 cites W1839188703 @default.
• W3046973944 cites W1910005513 @default.
• W3046973944 cites W1964232997 @default.
• W3046973944 cites W1968587972 @default.
• W3046973944 cites W1987146298 @default.
• W3046973944 cites W2007499410 @default.
• W3046973944 cites W2034583414 @default.
• W3046973944 cites W2038744035 @default.
• W3046973944 cites W2080418767 @default.
• W3046973944 cites W2128891846 @default.
• W3046973944 cites W2137579115 @default.
• W3046973944 cites W2138806334 @default.
• W3046973944 cites W2143108459 @default.
• W3046973944 cites W2159401023 @default.
• W3046973944 cites W2168031418 @default.
• W3046973944 cites W2169894204 @default.
• W3046973944 cites W2345399180 @default.
• W3046973944 cites W2487212353 @default.
• W3046973944 cites W2578061423 @default.
• W3046973944 cites W2594599876 @default.
• W3046973944 cites W2607768992 @default.
• W3046973944 cites W2611696724 @default.
• W3046973944 cites W2614567943 @default.
• W3046973944 cites W2623830099 @default.
• W3046973944 cites W2732056586 @default.
• W3046973944 cites W2782029005 @default.
• W3046973944 cites W2895877601 @default.
• W3046973944 cites W2900569176 @default.
• W3046973944 cites W2900756811 @default.
• W3046973944 cites W2908517774 @default.
• W3046973944 cites W2913334650 @default.
• W3046973944 cites W2913917535 @default.
• W3046973944 cites W2920869071 @default.
• W3046973944 cites W2927797227 @default.
• W3046973944 cites W2928305023 @default.
• W3046973944 cites W2945824415 @default.
• W3046973944 cites W2946916533 @default.
• W3046973944 cites W2958493709 @default.
• W3046973944 cites W4210471029 @default.
• W3046973944 doi "https://doi.org/10.1158/1541-7786.mcr-20-0078" @default.
• W3046973944 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7642188" @default.
• W3046973944 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32753475" @default.
• W3046973944 hasPublicationYear "2020" @default.
• W3046973944 type Work @default.
• W3046973944 sameAs 3046973944 @default.
• W3046973944 citedByCount "2" @default.
• W3046973944 countsByYear W30469739442021 @default.
• W3046973944 countsByYear W30469739442023 @default.
• W3046973944 crossrefType "journal-article" @default.
• W3046973944 hasAuthorship W3046973944A5005608989 @default.
• W3046973944 hasAuthorship W3046973944A5007639428 @default.
• W3046973944 hasAuthorship W3046973944A5037434863 @default.
• W3046973944 hasAuthorship W3046973944A5038705314 @default.
• W3046973944 hasAuthorship W3046973944A5040774134 @default.
• W3046973944 hasAuthorship W3046973944A5067688250 @default.
• W3046973944 hasAuthorship W3046973944A5073583842 @default.
• W3046973944 hasAuthorship W3046973944A5082346201 @default.
• W3046973944 hasAuthorship W3046973944A5089929964 @default.
• W3046973944 hasBestOaLocation W30469739441 @default.
• W3046973944 hasConcept C104317684 @default.
• W3046973944 hasConcept C145059251 @default.
• W3046973944 hasConcept C148738053 @default.
• W3046973944 hasConcept C162746124 @default.
• W3046973944 hasConcept C20875687 @default.
• W3046973944 hasConcept C28328180 @default.
• W3046973944 hasConcept C502942594 @default.
• W3046973944 hasConcept C55493867 @default.
• W3046973944 hasConcept C57074206 @default.
• W3046973944 hasConcept C62478195 @default.
• W3046973944 hasConcept C86803240 @default.
• W3046973944 hasConcept C95444343 @default.
• W3046973944 hasConceptScore W3046973944C104317684 @default.
• W3046973944 hasConceptScore W3046973944C145059251 @default.
• W3046973944 hasConceptScore W3046973944C148738053 @default.
• W3046973944 hasConceptScore W3046973944C162746124 @default.
• W3046973944 hasConceptScore W3046973944C20875687 @default.
• W3046973944 hasConceptScore W3046973944C28328180 @default.
• W3046973944 hasConceptScore W3046973944C502942594 @default.
• W3046973944 hasConceptScore W3046973944C55493867 @default.
• W3046973944 hasConceptScore W3046973944C57074206 @default.
• W3046973944 hasConceptScore W3046973944C62478195 @default.
• W3046973944 hasConceptScore W3046973944C86803240 @default.

• next