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• W3046977054 abstract "Cyclic peptides with engineered protein-binding activity have gained increasing attention for use in therapeutic and biotechnology applications. We describe the efficient isolation and characterization of cyclic peptide binders from genetically encoded combinatorial libraries using yeast surface display. Here, peptide cyclization is achieved by disuccinimidyl glutarate-mediated cross-linking of amine groups within a linear peptide sequence that is expressed as a yeast cell surface fusion. Using this approach, we first screened a library of cyclic heptapeptides using magnetic selection, followed by fluorescence activated cell sorting (FACS) to isolate binders for a model target (lysozyme) with low micromolar binding affinity (KD ∼ 1.2–3.7 μM). The isolated peptides bind lysozyme selectively and only when cyclized. Importantly, we showed that yeast surface displayed cyclic peptides can be used to efficiently obtain quantitative estimates of binding affinity, circumventing the need for chemical synthesis of the selected peptides. Subsequently, to demonstrate broader applicability of our approach, we isolated cyclic heptapeptides that bind human interleukin-17 (IL-17) using yeast-displayed IL-17 as a target for magnetic selection, followed by FACS using recombinant IL-17. Molecular docking simulations and follow-up experimental analyses identified a candidate cyclic peptide that likely binds IL-17 in its receptor binding region with moderate apparent affinity (KD ∼ 300 nM). Taken together, our results show that yeast surface display can be used to efficiently isolate and characterize cyclic peptides generated by chemical modification from combinatorial libraries." @default.
• W3046977054 created "2020-08-10" @default.
• W3046977054 creator A5023824047 @default.
• W3046977054 creator A5026794701 @default.
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• W3046977054 creator A5052722663 @default.
• W3046977054 creator A5055960888 @default.
• W3046977054 creator A5064012428 @default.
• W3046977054 date "2020-08-03" @default.
• W3046977054 modified "2023-10-16" @default.
• W3046977054 title "Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display" @default.
• W3046977054 cites W103571734 @default.
• W3046977054 cites W13307236 @default.
• W3046977054 cites W1512686244 @default.
• W3046977054 cites W1526206219 @default.
• W3046977054 cites W1584021532 @default.
• W3046977054 cites W1664785395 @default.
• W3046977054 cites W1969350101 @default.
• W3046977054 cites W1975304761 @default.
• W3046977054 cites W1979715704 @default.
• W3046977054 cites W1981300064 @default.
• W3046977054 cites W1982293452 @default.
• W3046977054 cites W1986184008 @default.
• W3046977054 cites W1989622506 @default.
• W3046977054 cites W1994757111 @default.
• W3046977054 cites W1995094552 @default.
• W3046977054 cites W1998233841 @default.
• W3046977054 cites W2001597478 @default.
• W3046977054 cites W2006241461 @default.
• W3046977054 cites W2006446936 @default.
• W3046977054 cites W2007109740 @default.
• W3046977054 cites W2014171031 @default.
• W3046977054 cites W2016583991 @default.
• W3046977054 cites W2021832765 @default.
• W3046977054 cites W2023018411 @default.
• W3046977054 cites W2024642459 @default.
• W3046977054 cites W2025116694 @default.
• W3046977054 cites W2028046413 @default.
• W3046977054 cites W2028493269 @default.
• W3046977054 cites W2030189518 @default.
• W3046977054 cites W2031168104 @default.
• W3046977054 cites W2031717394 @default.
• W3046977054 cites W2032299291 @default.
• W3046977054 cites W2037185463 @default.
• W3046977054 cites W2040347730 @default.
• W3046977054 cites W2041189078 @default.
• W3046977054 cites W2043438605 @default.
• W3046977054 cites W2043714541 @default.
• W3046977054 cites W2044721777 @default.
• W3046977054 cites W2053538195 @default.
• W3046977054 cites W2057305507 @default.
• W3046977054 cites W2059852435 @default.
• W3046977054 cites W2060542507 @default.
• W3046977054 cites W2065838297 @default.
• W3046977054 cites W2066837313 @default.
• W3046977054 cites W2070136617 @default.
• W3046977054 cites W2070360032 @default.
• W3046977054 cites W2072554880 @default.
• W3046977054 cites W2080356101 @default.
• W3046977054 cites W2084781832 @default.
• W3046977054 cites W2091245340 @default.
• W3046977054 cites W2093057097 @default.
• W3046977054 cites W2095719702 @default.
• W3046977054 cites W2096078734 @default.
• W3046977054 cites W2096391885 @default.
• W3046977054 cites W2097888801 @default.
• W3046977054 cites W2099580557 @default.
• W3046977054 cites W2106383417 @default.
• W3046977054 cites W2107409328 @default.
• W3046977054 cites W2114926334 @default.
• W3046977054 cites W2115513456 @default.
• W3046977054 cites W2121543947 @default.
• W3046977054 cites W2129842163 @default.
• W3046977054 cites W2135351284 @default.
• W3046977054 cites W2135586616 @default.
• W3046977054 cites W2138567388 @default.
• W3046977054 cites W2142350160 @default.
• W3046977054 cites W2142868265 @default.
• W3046977054 cites W2144842198 @default.
• W3046977054 cites W2145352278 @default.
• W3046977054 cites W2148893085 @default.
• W3046977054 cites W2153983456 @default.
• W3046977054 cites W2154019529 @default.
• W3046977054 cites W2158314009 @default.
• W3046977054 cites W2160259785 @default.
• W3046977054 cites W2166087321 @default.
• W3046977054 cites W2166765429 @default.
• W3046977054 cites W2170551524 @default.
• W3046977054 cites W2234017926 @default.
• W3046977054 cites W2275655681 @default.
• W3046977054 cites W2298564532 @default.
• W3046977054 cites W2314173824 @default.
• W3046977054 cites W2331643317 @default.
• W3046977054 cites W2397565578 @default.
• W3046977054 cites W2410221116 @default.
• W3046977054 cites W2554477349 @default.
• W3046977054 cites W2562506588 @default.
• W3046977054 cites W2565952096 @default.

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