FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3047129907> ?p ?o ?g. }

• W3047129907 abstract "Abstract Site-specific RNA functionalization is in high demand, but remains a challenge, particularly for RNAs produced by transcription rather than by total synthesis. Recent studies have described acylimidazole reagents that react in high yields at 2’-OH groups in RNAs. To date, the reactions occur stochastically at non-base-paired regions of RNA, covering much of the RNA in scattered acyl esters. Localized reactions, if possible, could prove useful in many applications, providing functional handles at specific sites and sequences of the biopolymer. Here we describe a DNA-directed strategy for in vitro functionalization of RNA at site-localized 2’-OH groups. The method, RNA Acylation at Induced Loops (RAIL), utilizes complementary helper DNA oligonucleotides that expose gaps or loops at selected positions while protecting the remainder in DNA-RNA duplexes. Reaction with acylimidazole reagents is then carried out, providing high yields of 2’-OH conjugation at predetermined sites. Subsequent removal of the DNA provides the RNA functionalized as desired. Experiments reveal optimal helper oligodeoxynucleotide designs and conditions for the reaction, and tests of the approach were carried out to control ribozyme activities and to label RNAs with dual-color fluorescent dyes. The RAIL approach offers a simple new strategy for site-specific labeling and controlling RNAs of any length and origin." @default.
• W3047129907 created "2020-08-10" @default.
• W3047129907 creator A5007860684 @default.
• W3047129907 creator A5047732965 @default.
• W3047129907 creator A5078529502 @default.
• W3047129907 date "2020-08-06" @default.
• W3047129907 modified "2023-10-02" @default.
• W3047129907 title "Site-specific RNA Functionalization via DNA-induced Structure" @default.
• W3047129907 cites W1640318440 @default.
• W3047129907 cites W1728081620 @default.
• W3047129907 cites W1822176298 @default.
• W3047129907 cites W1914929330 @default.
• W3047129907 cites W1973599988 @default.
• W3047129907 cites W1974454061 @default.
• W3047129907 cites W1978513278 @default.
• W3047129907 cites W1985790087 @default.
• W3047129907 cites W2008865120 @default.
• W3047129907 cites W2011400542 @default.
• W3047129907 cites W2026810966 @default.
• W3047129907 cites W2033741612 @default.
• W3047129907 cites W2040884830 @default.
• W3047129907 cites W2041366444 @default.
• W3047129907 cites W2053742705 @default.
• W3047129907 cites W2057957131 @default.
• W3047129907 cites W2058547049 @default.
• W3047129907 cites W2066444035 @default.
• W3047129907 cites W2066836230 @default.
• W3047129907 cites W2068205741 @default.
• W3047129907 cites W2071755348 @default.
• W3047129907 cites W2072608774 @default.
• W3047129907 cites W2083413121 @default.
• W3047129907 cites W2083543262 @default.
• W3047129907 cites W2087104623 @default.
• W3047129907 cites W2095594869 @default.
• W3047129907 cites W2099053774 @default.
• W3047129907 cites W2105381069 @default.
• W3047129907 cites W2105598902 @default.
• W3047129907 cites W2113231837 @default.
• W3047129907 cites W2120974004 @default.
• W3047129907 cites W2126881800 @default.
• W3047129907 cites W2128536845 @default.
• W3047129907 cites W2136534992 @default.
• W3047129907 cites W2146685096 @default.
• W3047129907 cites W2149809839 @default.
• W3047129907 cites W2315282308 @default.
• W3047129907 cites W2345270442 @default.
• W3047129907 cites W2388201280 @default.
• W3047129907 cites W2468469919 @default.
• W3047129907 cites W2495368390 @default.
• W3047129907 cites W2537962432 @default.
• W3047129907 cites W2607059961 @default.
• W3047129907 cites W2790726960 @default.
• W3047129907 cites W2792696004 @default.
• W3047129907 cites W2792898135 @default.
• W3047129907 cites W2796502596 @default.
• W3047129907 cites W2808369312 @default.
• W3047129907 cites W2886822966 @default.
• W3047129907 cites W2896600720 @default.
• W3047129907 cites W2899642980 @default.
• W3047129907 cites W2932122507 @default.
• W3047129907 cites W2953503489 @default.
• W3047129907 cites W2989663978 @default.
• W3047129907 cites W2990729484 @default.
• W3047129907 cites W2991644156 @default.
• W3047129907 cites W2997964087 @default.
• W3047129907 doi "https://doi.org/10.1101/2020.08.06.238576" @default.
• W3047129907 hasPublicationYear "2020" @default.
• W3047129907 type Work @default.
• W3047129907 sameAs 3047129907 @default.
• W3047129907 citedByCount "0" @default.
• W3047129907 crossrefType "posted-content" @default.
• W3047129907 hasAuthorship W3047129907A5007860684 @default.
• W3047129907 hasAuthorship W3047129907A5047732965 @default.
• W3047129907 hasAuthorship W3047129907A5078529502 @default.
• W3047129907 hasBestOaLocation W30471299071 @default.
• W3047129907 hasConcept C104317684 @default.
• W3047129907 hasConcept C115537861 @default.
• W3047129907 hasConcept C129312508 @default.
• W3047129907 hasConcept C138885662 @default.
• W3047129907 hasConcept C147789679 @default.
• W3047129907 hasConcept C161790260 @default.
• W3047129907 hasConcept C178790620 @default.
• W3047129907 hasConcept C179926584 @default.
• W3047129907 hasConcept C185592680 @default.
• W3047129907 hasConcept C193557335 @default.
• W3047129907 hasConcept C21951064 @default.
• W3047129907 hasConcept C40875361 @default.
• W3047129907 hasConcept C41895202 @default.
• W3047129907 hasConcept C552990157 @default.
• W3047129907 hasConcept C55493867 @default.
• W3047129907 hasConcept C67705224 @default.
• W3047129907 hasConcept C70721500 @default.
• W3047129907 hasConcept C76346623 @default.
• W3047129907 hasConcept C86803240 @default.
• W3047129907 hasConceptScore W3047129907C104317684 @default.
• W3047129907 hasConceptScore W3047129907C115537861 @default.
• W3047129907 hasConceptScore W3047129907C129312508 @default.
• W3047129907 hasConceptScore W3047129907C138885662 @default.
• W3047129907 hasConceptScore W3047129907C147789679 @default.
• W3047129907 hasConceptScore W3047129907C161790260 @default.

• next