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- W3047172301 abstract "Babesia bovis is closely related to the (sporozoan) parasites that cause malaria and toxoplasmosis in humans, and a successful (specific and broad-reaching) vaccine design has been hampered by the lack of understanding of sporozoan molecular genetics. The primary aim of this project was to investigate the molecular genetics of B. bovis. This was the first detailed analysis of nuclear genome organisation for a sporozoan other than for Plasmodium, and the study was successful in identifying new genes and sequence elements required for gene expression. The broader aim of the study was to identify the similarities and differences in sporozoan molecular genetics, and to understand the position in nature. As a result of the study, the molecular genetics of Sporozoa are predicted to be similar to other eukaryotes, yet significant differences are likely to exist between sporozoan genera. Molecular techniques were used to study genome organisation and gene expression in B. bovis. A 10.5 kb nuclear genome segment containing the vaccine candidate 12D3 antigen gene plus a large 5' flanking region was cloned and sequenced. In addition to the 12D3 gene, the segment is predicted to contain six previously undescribed genes for Babesia. The corresponding transcripts identified by Northern analyses, reverse transcription polymerase chain reaction (RT-PCR) and cDNA library screening were cloned and sequenced. Two of the gene products are novel to B. bovis. Four of the gene products contain ancient conserved regions and likely functions were inferred on this basis. The genes encode an L35 ribosomal protein homologue, an RNA-binding protein, a nucleoside monophosphate kinase and an ATP-binding protein. Predictions concerning the nuclear genome were made on the basis of the 10.5 kb segment, representing -0.1% of the genome. The predictions indicate that the genome of B. bovis has an average nucleotide composition of 61% A+T and encodes up to 5,600 genes. Properties such as high gene density, small intron size and the complex arrangement of genes on both DNA strands are consistent with B. bovis possessing a compact genome. Nearly 90% of the genome segment was transcribed. Six genes were transcribed monocistronically whilst some evidence was obtained suggesting one gene may be transcribed as a polycistronic messenger. Two sets of genes may also overlap at their 5' ends. Genome properties including gene density, nucleotide composition and intron structure were similar to the non-parasitic protozoan Saccharomyces cerevisiae and the sporozoan Theileria, and less similar to other sporozoan genera and higher eukaryotes." @default.
- W3047172301 created "2020-08-10" @default.
- W3047172301 creator A5077832490 @default.
- W3047172301 date "2020-06-30" @default.
- W3047172301 modified "2023-09-24" @default.
- W3047172301 title "Molecular genetics of Babesia bovis : nuclear genome organisation and gene expression" @default.
- W3047172301 doi "https://doi.org/10.14264/b9d2eb5" @default.
- W3047172301 hasPublicationYear "2020" @default.
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