FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3047441700> ?p ?o ?g. }

• W3047441700 endingPage "F551" @default.
• W3047441700 startingPage "F541" @default.
• W3047441700 abstract "Plasma phosphate (P i ) levels are tightly controlled, and elevated plasma P i levels are associated with an increased risk of cardiovascular complications and death. Two renal transport proteins mediate the majority of P i reabsorption: Na + -phosphate cotransporters Npt2a and Npt2c, with Npt2a accounting for 70–80% of P i reabsorption. The aim of the present study was to determine the in vitro effects of a novel Npt2a inhibitor (PF-06869206) in opossum kidney (OK) cells as well as determine its selectivity in vivo in Npt2a knockout (Npt2a −/− ) mice. In OK cells, Npt2a inhibitor caused dose-dependent reductions of Na + -dependent P i uptake (IC 50 : ~1.4 μmol/L), whereas the unselective Npt2 inhibitor phosphonoformic acid (PFA) resulted in an ~20% stronger inhibition of P i uptake. The dose-dependent inhibitory effects were present after 24 h of incubation with both low- and high-P i media. Michaelis-Menten kinetics in OK cells identified an ~2.4-fold higher K m for P i in response to Npt2a inhibition with no significant change in apparent V max . Higher parathyroid hormone concentrations decreased P i uptake equivalent to the maximal inhibitory effect of Npt2a inhibitor. In vivo, the Npt2a inhibitor induced a dose-dependent increase in urinary P i excretion in wild-type mice (ED 50 : ~23 mg/kg), which was completely absent in Npt2a −/− mice, alongside a lack of decrease in plasma P i . Of note, the Npt2a inhibitor-induced dose-dependent increase in urinary Na + excretion was still present in Npt2a −/− mice, a response possibly mediated by an off-target acute inhibitory effect of the Npt2a inhibitor on open probability of the epithelial Na + channel in the cortical collecting duct." @default.
• W3047441700 created "2020-08-10" @default.
• W3047441700 creator A5014657118 @default.
• W3047441700 creator A5019574921 @default.
• W3047441700 creator A5041014294 @default.
• W3047441700 creator A5058300732 @default.
• W3047441700 creator A5064579974 @default.
• W3047441700 creator A5091511300 @default.
• W3047441700 date "2020-09-01" @default.
• W3047441700 modified "2023-10-15" @default.
• W3047441700 title "PF-06869206 is a selective inhibitor of renal P_itransport: evidence from in vitro and in vivo studies" @default.
• W3047441700 cites W1573628824 @default.
• W3047441700 cites W1596631230 @default.
• W3047441700 cites W1912328376 @default.
• W3047441700 cites W1932664859 @default.
• W3047441700 cites W1967555998 @default.
• W3047441700 cites W1973638806 @default.
• W3047441700 cites W1989087528 @default.
• W3047441700 cites W1990709199 @default.
• W3047441700 cites W1997478832 @default.
• W3047441700 cites W2000513641 @default.
• W3047441700 cites W2000775104 @default.
• W3047441700 cites W2000967798 @default.
• W3047441700 cites W2003607999 @default.
• W3047441700 cites W2015668150 @default.
• W3047441700 cites W2025984691 @default.
• W3047441700 cites W2030174339 @default.
• W3047441700 cites W2045847718 @default.
• W3047441700 cites W2048466021 @default.
• W3047441700 cites W2051483612 @default.
• W3047441700 cites W2059629870 @default.
• W3047441700 cites W2070063902 @default.
• W3047441700 cites W2073196054 @default.
• W3047441700 cites W2079813075 @default.
• W3047441700 cites W2083288593 @default.
• W3047441700 cites W2085051313 @default.
• W3047441700 cites W2088477824 @default.
• W3047441700 cites W2091129466 @default.
• W3047441700 cites W2100820099 @default.
• W3047441700 cites W2106107456 @default.
• W3047441700 cites W2107207648 @default.
• W3047441700 cites W2109012519 @default.
• W3047441700 cites W2114669693 @default.
• W3047441700 cites W2115596685 @default.
• W3047441700 cites W2122944904 @default.
• W3047441700 cites W2127674924 @default.
• W3047441700 cites W2131395923 @default.
• W3047441700 cites W2131854193 @default.
• W3047441700 cites W2134391819 @default.
• W3047441700 cites W2134698248 @default.
• W3047441700 cites W2136991956 @default.
• W3047441700 cites W2139989191 @default.
• W3047441700 cites W2143657892 @default.
• W3047441700 cites W2161630557 @default.
• W3047441700 cites W2171005064 @default.
• W3047441700 cites W2348562354 @default.
• W3047441700 cites W2409598385 @default.
• W3047441700 cites W2412762867 @default.
• W3047441700 cites W2432466048 @default.
• W3047441700 cites W2589579829 @default.
• W3047441700 cites W2605277235 @default.
• W3047441700 cites W2763746713 @default.
• W3047441700 cites W2774948007 @default.
• W3047441700 cites W2791427360 @default.
• W3047441700 cites W2792605613 @default.
• W3047441700 cites W2793768780 @default.
• W3047441700 cites W2798066213 @default.
• W3047441700 cites W2888776119 @default.
• W3047441700 cites W2896372898 @default.
• W3047441700 cites W2899214267 @default.
• W3047441700 cites W2922795099 @default.
• W3047441700 cites W2948067051 @default.
• W3047441700 cites W2948505973 @default.
• W3047441700 cites W2967158864 @default.
• W3047441700 cites W2977682063 @default.
• W3047441700 cites W2980063695 @default.
• W3047441700 cites W3012227429 @default.
• W3047441700 doi "https://doi.org/10.1152/ajprenal.00146.2020" @default.
• W3047441700 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7509280" @default.
• W3047441700 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32744087" @default.
• W3047441700 hasPublicationYear "2020" @default.
• W3047441700 type Work @default.
• W3047441700 sameAs 3047441700 @default.
• W3047441700 citedByCount "10" @default.
• W3047441700 countsByYear W30474417002021 @default.
• W3047441700 countsByYear W30474417002022 @default.
• W3047441700 countsByYear W30474417002023 @default.
• W3047441700 crossrefType "journal-article" @default.
• W3047441700 hasAuthorship W3047441700A5014657118 @default.
• W3047441700 hasAuthorship W3047441700A5019574921 @default.
• W3047441700 hasAuthorship W3047441700A5041014294 @default.
• W3047441700 hasAuthorship W3047441700A5058300732 @default.
• W3047441700 hasAuthorship W3047441700A5064579974 @default.
• W3047441700 hasAuthorship W3047441700A5091511300 @default.
• W3047441700 hasBestOaLocation W30474417001 @default.
• W3047441700 hasConcept C126322002 @default.
• W3047441700 hasConcept C134018914 @default.
• W3047441700 hasConcept C207001950 @default.

• next