FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3047443676> ?p ?o ?g. }

• W3047443676 endingPage "189" @default.
• W3047443676 startingPage "181" @default.
• W3047443676 abstract "Extracellular ATP (eATP) is a potent singling molecule in activation of fish innate immunity while the molecular determinants for eATP release in fish were not completely understood. Connexin32 (Cx32) is a member of gap junction protein family that plays important immunological functions in mammals. However, the immune relevance of Cx32 and its role in ATP release in fish has not been investigated. Here, we identified, characterized three Cx32 isoform genes (Cx32.2, Cx32.2x and Cx32.7) from the Japanese flounder Paralichthys olivaceus, and investigated their role in inflammation-induced ATP release in fish. Expression analysis revealed that even though all the three Cx32 genes are constitutively expressed in all examined Japanese flounder tissues, Cx32.2 and Cx32.2x are dominantly expressed in liver, and Cx32.7 is highly expressed in intestine and head kidney macrophages. In addition, we showed that gene expression of all the three Cx32 isoforms was modulated by cAMP stimulation and inflammatory challenges. Furthermore, we revealed that Cx32 expression was upregulated in TNF-alpha overexpressed Japanese flounder FG-9307 cells. Moreover, overexpression of the three Cx32 isoforms significantly reduced the gene expression level of LPS-induced pro-inflammatory cytokine IL-8 and TNF-alpha, indicating that Cx32 is involved in modulating inflammatory response in fish. Finally, we showed that inflammation-induced ATP release was significantly increased in Cx32-overexpressed Japanese flounder FG-9307 cells, and this increased ATP release could be attenuated by pre-incubation with gap junction protein blocker carbenoxolone. Taken together, we for the first time reported the involvement of Cx32 in fish immunity. Our findings suggested that in addition to Cx43 and pannexin1 channels, Cx32 also plays a role in inflammation-induced ATP release in fish." @default.
• W3047443676 created "2020-08-10" @default.
• W3047443676 creator A5007612048 @default.
• W3047443676 creator A5010465278 @default.
• W3047443676 creator A5020654618 @default.
• W3047443676 creator A5043296622 @default.
• W3047443676 creator A5061698874 @default.
• W3047443676 creator A5091207696 @default.
• W3047443676 date "2020-11-01" @default.
• W3047443676 modified "2023-10-13" @default.
• W3047443676 title "Characterization of three connexin32 genes and their role in inflammation-induced ATP release in the Japanese flounder Paralichthys olivaceus" @default.
• W3047443676 cites W1589379532 @default.
• W3047443676 cites W1769687268 @default.
• W3047443676 cites W1965070595 @default.
• W3047443676 cites W1977869287 @default.
• W3047443676 cites W1986144590 @default.
• W3047443676 cites W2008275141 @default.
• W3047443676 cites W2012654459 @default.
• W3047443676 cites W2032565394 @default.
• W3047443676 cites W2041221777 @default.
• W3047443676 cites W2051465829 @default.
• W3047443676 cites W2057077884 @default.
• W3047443676 cites W2057984382 @default.
• W3047443676 cites W2084877421 @default.
• W3047443676 cites W2090876087 @default.
• W3047443676 cites W2141752309 @default.
• W3047443676 cites W2146046916 @default.
• W3047443676 cites W2250409198 @default.
• W3047443676 cites W2330314962 @default.
• W3047443676 cites W2414052407 @default.
• W3047443676 cites W2473514416 @default.
• W3047443676 cites W2549327669 @default.
• W3047443676 cites W2605296449 @default.
• W3047443676 cites W2618435273 @default.
• W3047443676 cites W2627012524 @default.
• W3047443676 cites W2884211436 @default.
• W3047443676 cites W2917448503 @default.
• W3047443676 cites W2944305626 @default.
• W3047443676 cites W3000383616 @default.
• W3047443676 doi "https://doi.org/10.1016/j.fsi.2020.07.066" @default.
• W3047443676 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32768708" @default.
• W3047443676 hasPublicationYear "2020" @default.
• W3047443676 type Work @default.
• W3047443676 sameAs 3047443676 @default.
• W3047443676 citedByCount "7" @default.
• W3047443676 countsByYear W30474436762021 @default.
• W3047443676 countsByYear W30474436762022 @default.
• W3047443676 countsByYear W30474436762023 @default.
• W3047443676 crossrefType "journal-article" @default.
• W3047443676 hasAuthorship W3047443676A5007612048 @default.
• W3047443676 hasAuthorship W3047443676A5010465278 @default.
• W3047443676 hasAuthorship W3047443676A5020654618 @default.
• W3047443676 hasAuthorship W3047443676A5043296622 @default.
• W3047443676 hasAuthorship W3047443676A5061698874 @default.
• W3047443676 hasAuthorship W3047443676A5091207696 @default.
• W3047443676 hasConcept C136449434 @default.
• W3047443676 hasConcept C153911025 @default.
• W3047443676 hasConcept C158157758 @default.
• W3047443676 hasConcept C203014093 @default.
• W3047443676 hasConcept C2776070231 @default.
• W3047443676 hasConcept C2776367567 @default.
• W3047443676 hasConcept C2776533291 @default.
• W3047443676 hasConcept C2776914184 @default.
• W3047443676 hasConcept C2909208804 @default.
• W3047443676 hasConcept C505870484 @default.
• W3047443676 hasConcept C79879829 @default.
• W3047443676 hasConcept C86803240 @default.
• W3047443676 hasConcept C8891405 @default.
• W3047443676 hasConcept C95444343 @default.
• W3047443676 hasConceptScore W3047443676C136449434 @default.
• W3047443676 hasConceptScore W3047443676C153911025 @default.
• W3047443676 hasConceptScore W3047443676C158157758 @default.
• W3047443676 hasConceptScore W3047443676C203014093 @default.
• W3047443676 hasConceptScore W3047443676C2776070231 @default.
• W3047443676 hasConceptScore W3047443676C2776367567 @default.
• W3047443676 hasConceptScore W3047443676C2776533291 @default.
• W3047443676 hasConceptScore W3047443676C2776914184 @default.
• W3047443676 hasConceptScore W3047443676C2909208804 @default.
• W3047443676 hasConceptScore W3047443676C505870484 @default.
• W3047443676 hasConceptScore W3047443676C79879829 @default.
• W3047443676 hasConceptScore W3047443676C86803240 @default.
• W3047443676 hasConceptScore W3047443676C8891405 @default.
• W3047443676 hasConceptScore W3047443676C95444343 @default.
• W3047443676 hasFunder F4320323993 @default.
• W3047443676 hasFunder F4320326709 @default.
• W3047443676 hasLocation W30474436761 @default.
• W3047443676 hasOpenAccess W3047443676 @default.
• W3047443676 hasPrimaryLocation W30474436761 @default.
• W3047443676 hasRelatedWork W2040715146 @default.
• W3047443676 hasRelatedWork W2080940408 @default.
• W3047443676 hasRelatedWork W2155668182 @default.
• W3047443676 hasRelatedWork W2253876867 @default.
• W3047443676 hasRelatedWork W2316646150 @default.
• W3047443676 hasRelatedWork W2326645750 @default.
• W3047443676 hasRelatedWork W2335752955 @default.
• W3047443676 hasRelatedWork W2382227936 @default.
• W3047443676 hasRelatedWork W2592491021 @default.
• W3047443676 hasRelatedWork W2897603805 @default.

• next