FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3047603755> ?p ?o ?g. }

• W3047603755 abstract "Legionella pneumophila (L. pneumophila) is a gram-negative bacterium found ubiquitously in natural water sources where it replicates within amoebae, such as Acanthamoeba castellanii (A. castellanii). The evolution in amoebal hosts allows L. pneumophila to ‘accidentally’ infect human lungs after inhalation of aerosols containing L. pneumophila. In both human macrophages and amoebae, L. pneumophila replicates intracellularly within a vacuole known as the Legionella containing vacuole (LCV) that avoids fusion with the endocytic pathway. Fundamental to this process is the translocation of over 330 proteins by L. pneumophila into host cells by the Dot/Icm type 4 secretion system. To date, only a subset of Dot/Icm effector proteins has been characterised. These proteins are involved in manipulating host cellular processes such as ER vesicle recruitment, post-translational modifications and host cell survival. The majority of L. pneumophila effector proteins remain uncharacterised due to functional redundancy of effector proteins and complex effector protein regulation. In addition, despite the ecological and evolutionary significance of the intra-A. castellanii stage of the L. pneumophila life cycle, very little is known about this host-pathogen interaction. In this study, we constructed in-frame markerless mutants in L. pneumophila 130b according to genomic regions enriched for putative Dot/Icm effector genes and revealed host-specific genomic regions that are required for optimal L. pneumophila replication in A. castellanii. Specifically, by identifying a putative glutamate transporter necessary for replication in A. castellanii, but not in macrophages, this study suggests an aspect of L. pneumophila metabolism adaptation in different hosts.A. castellanii presents two life stages: active trophozoites and dormant cysts. Trophozoites act as reservoirs for L. pneumophila and are hijacked by L. pneumophila for bacterial replication. Cysts are resistant to anti-microbial treatments and were previously thought to facilitate L. pneumophila persistence and dissemination. However, in this study, we showed that the infection of A. castellanii with L. pneumophila led to inhibition of the transition from trophozoites to cysts in a Dot/Icm dependent manner. This inhibition partially required the gene letA, which encodes the regulator of two-component system LetAS. Pathogenic amoeba cysts prose a human health problem. This study provides a biological model for anti-cyst research and provides clues into critical encystment pathways.L. pneumophila can interfere with a broad range of host cellular pathways to establish the replication niche. Here, we investigate A. castellanii transcriptional response with L. pneumophila infection by RNA sequencing analysis. As a result, A. castellanii genes associated with mitosis, DNA replication and cell wall synthesis were significantly downregulated at late infection phase, which might contribute to the inhibition of A. castellanii proliferation and encystment. Compared with transcriptional profile in macrophage, L. pneumophila displayed different regulation on host eEF1a expression and genes associated with ATP production. Furthermore, a gene encoding sirtuin family protein, Sir6f, was upregulated during L. pneumophila infection, and silencing of sir6f led to decreased bacterial replication, suggesting that Sir6f is a host factor that facilitates L. pneumophila replication in A. castellanii. Evolution of L. pneumophila in amoebal hosts is hypothesized to allow its adaptations to survive in macrophages. Overall, this study highlights a number of strategies utilised by L. pneumophila during A. castellanii infection, including host-specific virulence factors, inhibition of A. castellanii encystment, affect host transcriptome and hijack of host factors. These microbial strategies will add valuable insights and provide potential targets regarding the development of new mechanisms for L. pneumophila control and prevention." @default.
• W3047603755 created "2020-08-10" @default.
• W3047603755 creator A5014394467 @default.
• W3047603755 date "2020-01-01" @default.
• W3047603755 modified "2023-09-27" @default.
• W3047603755 title "Amoebae as a host for Legionella replication" @default.
• W3047603755 hasPublicationYear "2020" @default.
• W3047603755 type Work @default.
• W3047603755 sameAs 3047603755 @default.
• W3047603755 citedByCount "0" @default.
• W3047603755 crossrefType "dissertation" @default.
• W3047603755 hasAuthorship W3047603755A5014394467 @default.
• W3047603755 hasConcept C102568950 @default.
• W3047603755 hasConcept C190062978 @default.
• W3047603755 hasConcept C2776118409 @default.
• W3047603755 hasConcept C2779164008 @default.
• W3047603755 hasConcept C51785407 @default.
• W3047603755 hasConcept C523546767 @default.
• W3047603755 hasConcept C54355233 @default.
• W3047603755 hasConcept C86803240 @default.
• W3047603755 hasConcept C89423630 @default.
• W3047603755 hasConcept C95444343 @default.
• W3047603755 hasConceptScore W3047603755C102568950 @default.
• W3047603755 hasConceptScore W3047603755C190062978 @default.
• W3047603755 hasConceptScore W3047603755C2776118409 @default.
• W3047603755 hasConceptScore W3047603755C2779164008 @default.
• W3047603755 hasConceptScore W3047603755C51785407 @default.
• W3047603755 hasConceptScore W3047603755C523546767 @default.
• W3047603755 hasConceptScore W3047603755C54355233 @default.
• W3047603755 hasConceptScore W3047603755C86803240 @default.
• W3047603755 hasConceptScore W3047603755C89423630 @default.
• W3047603755 hasConceptScore W3047603755C95444343 @default.
• W3047603755 hasLocation W30476037551 @default.
• W3047603755 hasOpenAccess W3047603755 @default.
• W3047603755 hasPrimaryLocation W30476037551 @default.
• W3047603755 hasRelatedWork W1513321252 @default.
• W3047603755 hasRelatedWork W1971510761 @default.
• W3047603755 hasRelatedWork W1981180892 @default.
• W3047603755 hasRelatedWork W1999355125 @default.
• W3047603755 hasRelatedWork W2004101686 @default.
• W3047603755 hasRelatedWork W2040667795 @default.
• W3047603755 hasRelatedWork W2091390126 @default.
• W3047603755 hasRelatedWork W2107539594 @default.
• W3047603755 hasRelatedWork W2116612937 @default.
• W3047603755 hasRelatedWork W2156223995 @default.
• W3047603755 hasRelatedWork W2169600942 @default.
• W3047603755 hasRelatedWork W2359718201 @default.
• W3047603755 hasRelatedWork W2548792267 @default.
• W3047603755 hasRelatedWork W2769755511 @default.
• W3047603755 hasRelatedWork W2887303977 @default.
• W3047603755 hasRelatedWork W2974855504 @default.
• W3047603755 hasRelatedWork W3035394415 @default.
• W3047603755 hasRelatedWork W3111713397 @default.
• W3047603755 hasRelatedWork W3132780031 @default.
• W3047603755 hasRelatedWork W4225979795 @default.
• W3047603755 isParatext "false" @default.
• W3047603755 isRetracted "false" @default.
• W3047603755 magId "3047603755" @default.
• W3047603755 workType "dissertation" @default.