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- W3047988077 endingPage "1160" @default.
- W3047988077 startingPage "1160" @default.
- W3047988077 abstract "The ribonome interconnects the proteome and the transcriptome. Specific biology is situated at this interface, which can be studied in bulk using omics approaches or specifically by targeting an individual protein or RNA species. In this review, we focus on both RNA- and ribonucleoprotein-(RNP) centric methods. These methods can be used to study the dynamics of the ribonome in response to a stimulus or to identify the proteins that interact with a specific RNA species. The purpose of this review is to provide and discuss an overview of strategies to cross-link RNA to proteins and the currently available RNA- and RNP-centric approaches to study RNPs. We elaborate on some major challenges common to most methods, involving RNP yield, purity and experimental cost. We identify the origin of these difficulties and propose to combine existing approaches to overcome these challenges. The solutions provided build on the recently developed organic phase separation protocols, such as Cross-Linked RNA eXtraction (XRNAX), orthogonal organic phase separation (OOPS) and Phenol-Toluol extraction (PTex)." @default.
- W3047988077 created "2020-08-13" @default.
- W3047988077 creator A5038561351 @default.
- W3047988077 creator A5042890277 @default.
- W3047988077 creator A5057161421 @default.
- W3047988077 date "2020-08-07" @default.
- W3047988077 modified "2023-10-15" @default.
- W3047988077 title "Single and Combined Methods to Specifically or Bulk-Purify RNA–Protein Complexes" @default.
- W3047988077 cites W1177610565 @default.
- W3047988077 cites W133826215 @default.
- W3047988077 cites W1746621838 @default.
- W3047988077 cites W176288343 @default.
- W3047988077 cites W1969161156 @default.
- W3047988077 cites W1974880213 @default.
- W3047988077 cites W1977076773 @default.
- W3047988077 cites W1979768717 @default.
- W3047988077 cites W1985468501 @default.
- W3047988077 cites W1986911150 @default.
- W3047988077 cites W1987197853 @default.
- W3047988077 cites W1992212213 @default.
- W3047988077 cites W1994910752 @default.
- W3047988077 cites W1997535872 @default.
- W3047988077 cites W2000391476 @default.
- W3047988077 cites W2002679195 @default.
- W3047988077 cites W2015175027 @default.
- W3047988077 cites W2019522436 @default.
- W3047988077 cites W2024244702 @default.
- W3047988077 cites W2025075195 @default.
- W3047988077 cites W2030974598 @default.
- W3047988077 cites W2032640007 @default.
- W3047988077 cites W2033509582 @default.
- W3047988077 cites W2037423297 @default.
- W3047988077 cites W2040356728 @default.
- W3047988077 cites W2041440237 @default.
- W3047988077 cites W2041770101 @default.
- W3047988077 cites W2044283421 @default.
- W3047988077 cites W2053758959 @default.
- W3047988077 cites W2053998703 @default.
- W3047988077 cites W2058303505 @default.
- W3047988077 cites W2059756152 @default.
- W3047988077 cites W2060203628 @default.
- W3047988077 cites W2072762554 @default.
- W3047988077 cites W2077527436 @default.
- W3047988077 cites W2089717004 @default.
- W3047988077 cites W2094652474 @default.
- W3047988077 cites W2113955947 @default.
- W3047988077 cites W2114824080 @default.
- W3047988077 cites W2125741255 @default.
- W3047988077 cites W2136724086 @default.
- W3047988077 cites W2137513571 @default.
- W3047988077 cites W2137969903 @default.
- W3047988077 cites W2141157874 @default.
- W3047988077 cites W2144137532 @default.
- W3047988077 cites W2145662716 @default.
- W3047988077 cites W2146372143 @default.
- W3047988077 cites W2152623715 @default.
- W3047988077 cites W2153114644 @default.
- W3047988077 cites W2154786191 @default.
- W3047988077 cites W2163677890 @default.
- W3047988077 cites W2163850141 @default.
- W3047988077 cites W2166229810 @default.
- W3047988077 cites W2167728354 @default.
- W3047988077 cites W2178708565 @default.
- W3047988077 cites W2182726182 @default.
- W3047988077 cites W2220329357 @default.
- W3047988077 cites W2257857567 @default.
- W3047988077 cites W2311921258 @default.
- W3047988077 cites W2326879335 @default.
- W3047988077 cites W2337834530 @default.
- W3047988077 cites W2343356574 @default.
- W3047988077 cites W2398358518 @default.
- W3047988077 cites W2460436629 @default.
- W3047988077 cites W2464741734 @default.
- W3047988077 cites W2470137797 @default.
- W3047988077 cites W2487752912 @default.
- W3047988077 cites W2514595599 @default.
- W3047988077 cites W2514803014 @default.
- W3047988077 cites W2520875945 @default.
- W3047988077 cites W2531933060 @default.
- W3047988077 cites W2538746205 @default.
- W3047988077 cites W2546956694 @default.
- W3047988077 cites W2561700798 @default.
- W3047988077 cites W2571952481 @default.
- W3047988077 cites W2604778851 @default.
- W3047988077 cites W2610260244 @default.
- W3047988077 cites W2643811244 @default.
- W3047988077 cites W2726257020 @default.
- W3047988077 cites W2752698886 @default.
- W3047988077 cites W2769941852 @default.
- W3047988077 cites W2776399449 @default.
- W3047988077 cites W2785329615 @default.
- W3047988077 cites W2786230719 @default.
- W3047988077 cites W2789834529 @default.
- W3047988077 cites W2809050290 @default.
- W3047988077 cites W2810359426 @default.
- W3047988077 cites W2894828038 @default.
- W3047988077 cites W2894897551 @default.
- W3047988077 cites W2897333681 @default.