FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048003079> ?p ?o ?g. }

• W3048003079 abstract "Abstract There is an urgent need for an accurate antibody test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we have developed 3 ELISA methods, trimer spike IgA, trimer spike IgG, and nucleocapsid IgG, for detecting anti-SARS-CoV-2 antibodies. We evaluated their performance in comparison with four commercial ELISAs, EDI™ Novel Coronavirus COVID-19 ELISA IgG and IgM, Euroimmun Anti-SARS-CoV-2 ELISA IgG and IgA, and one lateral flow assay, DPP® COVID-19 IgM/IgG System (Chembio). Both sensitivity and specificity were evaluated and the causes of false-positive reactions were determined. The assays were compared using 300 pre-epidemic samples and 100 PCR-confirmed COVID-19 samples. The sensitivities and specificities of the assays were as follows: 90%/100% (in-house trimer spike IgA), 90%/99.3% (in-house trimer spike IgG), 89%/98.3% (in-house nucleocapsid IgG), 73.7%/100% (EDI nucleocapsid IgM), 84.5%/95.1% (EDI nucleocapsid IgG), 95%/93.7% (Euroimmun S1 IgA), 82.8%/99.7% (Euroimmun S1 IgG), 82.0%/91.7% (Chembio nucleocapsid IgM), 92%/93.3% (Chembio nucleocapsid IgG). The presumed causes of positive signals from pre-epidemic samples in commercial and in-house assays were mixed. In some cases, positivity varied with assay repetition. In other cases, reactivity was abrogated by competitive inhibition (spiking the sample with analyte prior to performing the assay). In other cases, reactivity was consistently detected but not abrogated by analyte spiking. Overall, there was wide variability in assay performance using our samples, with in-house tests exhibiting the highest combined sensitivity and specificity. The causes of “false positivity” in pre-epidemic samples may be due to plasma antibodies apparently reacting with the analyte, or spurious reactivity may be directed against non-specific components in the assay system. Identification of these targets will be essential to improving assay performance." @default.
• W3048003079 created "2020-08-13" @default.
• W3048003079 creator A5017392719 @default.
• W3048003079 creator A5022617156 @default.
• W3048003079 creator A5030545878 @default.
• W3048003079 creator A5034495835 @default.
• W3048003079 creator A5040769857 @default.
• W3048003079 creator A5040770925 @default.
• W3048003079 creator A5043752885 @default.
• W3048003079 creator A5049799410 @default.
• W3048003079 creator A5056535179 @default.
• W3048003079 creator A5073854380 @default.
• W3048003079 date "2020-08-07" @default.
• W3048003079 modified "2023-09-25" @default.
• W3048003079 title "Specificity and Performance of Nucleocapsid and Spike-based SARS-CoV-2 Serologic Assays" @default.
• W3048003079 cites W1662940398 @default.
• W3048003079 cites W2115484790 @default.
• W3048003079 cites W2152575748 @default.
• W3048003079 cites W2156819923 @default.
• W3048003079 cites W2165671198 @default.
• W3048003079 cites W2612594162 @default.
• W3048003079 cites W3003217347 @default.
• W3048003079 cites W3012540309 @default.
• W3048003079 cites W3015940872 @default.
• W3048003079 cites W3018166943 @default.
• W3048003079 cites W3018638113 @default.
• W3048003079 cites W3021431776 @default.
• W3048003079 cites W3021590963 @default.
• W3048003079 cites W3024972049 @default.
• W3048003079 cites W3027576812 @default.
• W3048003079 doi "https://doi.org/10.1101/2020.08.05.20168476" @default.
• W3048003079 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7418763" @default.
• W3048003079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32793933" @default.
• W3048003079 hasPublicationYear "2020" @default.
• W3048003079 type Work @default.
• W3048003079 sameAs 3048003079 @default.
• W3048003079 citedByCount "15" @default.
• W3048003079 countsByYear W30480030792020 @default.
• W3048003079 countsByYear W30480030792021 @default.
• W3048003079 countsByYear W30480030792022 @default.
• W3048003079 countsByYear W30480030792023 @default.
• W3048003079 crossrefType "posted-content" @default.
• W3048003079 hasAuthorship W3048003079A5017392719 @default.
• W3048003079 hasAuthorship W3048003079A5022617156 @default.
• W3048003079 hasAuthorship W3048003079A5030545878 @default.
• W3048003079 hasAuthorship W3048003079A5034495835 @default.
• W3048003079 hasAuthorship W3048003079A5040769857 @default.
• W3048003079 hasAuthorship W3048003079A5040770925 @default.
• W3048003079 hasAuthorship W3048003079A5043752885 @default.
• W3048003079 hasAuthorship W3048003079A5049799410 @default.
• W3048003079 hasAuthorship W3048003079A5056535179 @default.
• W3048003079 hasAuthorship W3048003079A5073854380 @default.
• W3048003079 hasBestOaLocation W30480030791 @default.
• W3048003079 hasConcept C10390740 @default.
• W3048003079 hasConcept C142724271 @default.
• W3048003079 hasConcept C159047783 @default.
• W3048003079 hasConcept C159654299 @default.
• W3048003079 hasConcept C178790620 @default.
• W3048003079 hasConcept C185592680 @default.
• W3048003079 hasConcept C203014093 @default.
• W3048003079 hasConcept C2776403692 @default.
• W3048003079 hasConcept C2776553844 @default.
• W3048003079 hasConcept C2777648638 @default.
• W3048003079 hasConcept C2779134260 @default.
• W3048003079 hasConcept C2779546866 @default.
• W3048003079 hasConcept C2780898057 @default.
• W3048003079 hasConcept C2909509916 @default.
• W3048003079 hasConcept C3007834351 @default.
• W3048003079 hasConcept C3008058167 @default.
• W3048003079 hasConcept C43617362 @default.
• W3048003079 hasConcept C45189115 @default.
• W3048003079 hasConcept C524204448 @default.
• W3048003079 hasConcept C71924100 @default.
• W3048003079 hasConceptScore W3048003079C10390740 @default.
• W3048003079 hasConceptScore W3048003079C142724271 @default.
• W3048003079 hasConceptScore W3048003079C159047783 @default.
• W3048003079 hasConceptScore W3048003079C159654299 @default.
• W3048003079 hasConceptScore W3048003079C178790620 @default.
• W3048003079 hasConceptScore W3048003079C185592680 @default.
• W3048003079 hasConceptScore W3048003079C203014093 @default.
• W3048003079 hasConceptScore W3048003079C2776403692 @default.
• W3048003079 hasConceptScore W3048003079C2776553844 @default.
• W3048003079 hasConceptScore W3048003079C2777648638 @default.
• W3048003079 hasConceptScore W3048003079C2779134260 @default.
• W3048003079 hasConceptScore W3048003079C2779546866 @default.
• W3048003079 hasConceptScore W3048003079C2780898057 @default.
• W3048003079 hasConceptScore W3048003079C2909509916 @default.
• W3048003079 hasConceptScore W3048003079C3007834351 @default.
• W3048003079 hasConceptScore W3048003079C3008058167 @default.
• W3048003079 hasConceptScore W3048003079C43617362 @default.
• W3048003079 hasConceptScore W3048003079C45189115 @default.
• W3048003079 hasConceptScore W3048003079C524204448 @default.
• W3048003079 hasConceptScore W3048003079C71924100 @default.
• W3048003079 hasLocation W30480030791 @default.
• W3048003079 hasLocation W30480030792 @default.
• W3048003079 hasOpenAccess W3048003079 @default.
• W3048003079 hasPrimaryLocation W30480030791 @default.
• W3048003079 hasRelatedWork W2099244599 @default.
• W3048003079 hasRelatedWork W2105300830 @default.
• W3048003079 hasRelatedWork W3013048436 @default.

• next