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- W3048007410 abstract "Spectrally separated fluorophores allow the observation of multiple targets simultaneously inside living cells, leading to a deeper understanding of the molecular interplay that regulates cell function and fate. Chemogenetic systems combining a tag and a synthetic fluorophore provide certain advantages over fluorescent proteins since there is no requirement for chromophore maturation. Here, we present the engineering of a set of spectrally orthogonal fluorogen-activating tags based on the fluorescence-activating and absorption shifting tag (FAST) that are compatible with two-color, live-cell imaging. The resulting tags, greenFAST and redFAST, demonstrate orthogonality not only in their fluorogen recognition capabilities, but also in their one- and two-photon absorption profiles. This pair of orthogonal tags allowed the creation of a two-color cell cycle sensor capable of detecting very short, early cell cycles in zebrafish development and the development of split complementation systems capable of detecting multiple protein-protein interactions by live-cell fluorescence microscopy." @default.
- W3048007410 created "2020-08-13" @default.
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- W3048007410 date "2020-08-10" @default.
- W3048007410 modified "2023-10-10" @default.
- W3048007410 title "Orthogonal fluorescent chemogenetic reporters for multicolor imaging" @default.
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- W3048007410 doi "https://doi.org/10.1038/s41589-020-0611-0" @default.
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