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• W3048122913 startingPage "119739" @default.
• W3048122913 abstract "A key consideration in the clinical translation of nanomedicines is determining their in vivo biodistribution in preclinical studies, which is important for predicting and correlating therapeutic efficacy and safety. There are a number of techniques available for analyzing the in vivo biodistribution of nanoparticles, with each having its own advantages and limitations. However, conventional techniques are limited by their inability to image the three-dimensional (3D) association of nanoparticles with cells, vasculature and other biological structures in whole organs at a subcellular level. Recently, optical clearing techniques have been used to evaluate the biodistribution of nanoparticles by 3D organ imaging. Optical clearing is a procedure that is increasingly being used to improve the imaging of biological tissues, whereby light scattering substances are removed to better match the refractive indices of different tissue layers. The use of optical clearing techniques has the potential to transform the way we evaluate the biodistribution of new and existing nanomedicines, as it allows the visualization of the interaction of nanoparticles with the biological environment in intact tissues. This review will compare the main optical clearing techniques and will address the considerations for the use of these techniques to evaluate nanoparticle biodistribution." @default.
• W3048122913 created "2020-08-13" @default.
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• W3048122913 date "2020-10-01" @default.
• W3048122913 modified "2023-10-12" @default.
• W3048122913 title "Considerations for using optical clearing techniques for 3D imaging of nanoparticle biodistribution" @default.
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• W3048122913 doi "https://doi.org/10.1016/j.ijpharm.2020.119739" @default.
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