Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048155269> ?p ?o ?g. }
- W3048155269 endingPage "3593" @default.
- W3048155269 startingPage "3580" @default.
- W3048155269 abstract "Abstract Formylglycine‐generating enzymes specifically oxidize cysteine within the consensus sequence CxPxR to C α ‐formylglycine (FGly). This noncanonical electrophilic amino acid can subsequently be addressed selectively by bioorthogonal hydrazino‐ iso ‐Pictet‐Spengler (HIPS) or Knoevenagel ligation to attach payloads like fluorophores or drugs to proteins to obtain a defined payload‐to‐protein ratio. However, the disadvantages of these conjugation techniques include the need for a large excess of conjugation building block, comparably low reaction rates and limited stability of FGly‐containing proteins. Therefore, functionalized clickable HIPS and tandem Knoevenagel building blocks were synthesized, conjugated to small proteins (DARPins) and subsequently linked to strained alkyne‐containing payloads for protein labeling. This procedure allowed the selective bioconjugation of one or two DBCO‐carrying payloads with nearly stoichiometric amounts at low concentrations. Furthermore, an azide‐modified tandem Knoevenagel building block enabled the synthesis of branched PEG linkers and the conjugation of two fluorophores, resulting in an improved signal‐to‐noise ratio in live‐cell fluorescence‐imaging experiments targeting the EGF receptor." @default.
- W3048155269 created "2020-08-13" @default.
- W3048155269 creator A5009571345 @default.
- W3048155269 creator A5013810790 @default.
- W3048155269 creator A5031475047 @default.
- W3048155269 creator A5031744284 @default.
- W3048155269 creator A5068192515 @default.
- W3048155269 creator A5077845134 @default.
- W3048155269 creator A5084111609 @default.
- W3048155269 date "2020-09-18" @default.
- W3048155269 modified "2023-10-18" @default.
- W3048155269 title "Bifunctional Reagents for Formylglycine Conjugation: Pitfalls and Breakthroughs" @default.
- W3048155269 cites W1670498114 @default.
- W3048155269 cites W1868869536 @default.
- W3048155269 cites W1923025228 @default.
- W3048155269 cites W1969360694 @default.
- W3048155269 cites W1974414796 @default.
- W3048155269 cites W1975140798 @default.
- W3048155269 cites W1978513278 @default.
- W3048155269 cites W1983639577 @default.
- W3048155269 cites W1984111577 @default.
- W3048155269 cites W1989223590 @default.
- W3048155269 cites W1997537633 @default.
- W3048155269 cites W2001444550 @default.
- W3048155269 cites W2009510047 @default.
- W3048155269 cites W2010621614 @default.
- W3048155269 cites W2022897757 @default.
- W3048155269 cites W2023753056 @default.
- W3048155269 cites W2029661897 @default.
- W3048155269 cites W2032021124 @default.
- W3048155269 cites W2034184453 @default.
- W3048155269 cites W2034403740 @default.
- W3048155269 cites W2039730742 @default.
- W3048155269 cites W2043825890 @default.
- W3048155269 cites W2060746246 @default.
- W3048155269 cites W2064352582 @default.
- W3048155269 cites W2070720042 @default.
- W3048155269 cites W2072471477 @default.
- W3048155269 cites W2086003789 @default.
- W3048155269 cites W2087893133 @default.
- W3048155269 cites W2089082987 @default.
- W3048155269 cites W2090371323 @default.
- W3048155269 cites W2097114111 @default.
- W3048155269 cites W2111010990 @default.
- W3048155269 cites W2121942664 @default.
- W3048155269 cites W2122625654 @default.
- W3048155269 cites W2134152126 @default.
- W3048155269 cites W2145912253 @default.
- W3048155269 cites W2146913917 @default.
- W3048155269 cites W2151709132 @default.
- W3048155269 cites W2152707194 @default.
- W3048155269 cites W2158381930 @default.
- W3048155269 cites W2165954990 @default.
- W3048155269 cites W2172121653 @default.
- W3048155269 cites W2322951142 @default.
- W3048155269 cites W2325871243 @default.
- W3048155269 cites W2326085690 @default.
- W3048155269 cites W2327630133 @default.
- W3048155269 cites W2341028562 @default.
- W3048155269 cites W2417897260 @default.
- W3048155269 cites W2468409204 @default.
- W3048155269 cites W2516624091 @default.
- W3048155269 cites W2551998204 @default.
- W3048155269 cites W2731987565 @default.
- W3048155269 cites W2732018881 @default.
- W3048155269 cites W2743985231 @default.
- W3048155269 cites W2785821396 @default.
- W3048155269 cites W2789550049 @default.
- W3048155269 cites W2793484199 @default.
- W3048155269 cites W2794201919 @default.
- W3048155269 cites W2802923922 @default.
- W3048155269 cites W2808751881 @default.
- W3048155269 cites W2901226998 @default.
- W3048155269 cites W2903485693 @default.
- W3048155269 cites W2909708488 @default.
- W3048155269 cites W2910492756 @default.
- W3048155269 cites W2914858703 @default.
- W3048155269 cites W2931897757 @default.
- W3048155269 cites W2953265721 @default.
- W3048155269 cites W3004668339 @default.
- W3048155269 cites W4205980955 @default.
- W3048155269 cites W4206270724 @default.
- W3048155269 cites W4210472753 @default.
- W3048155269 cites W4231385139 @default.
- W3048155269 cites W4239203788 @default.
- W3048155269 cites W4242773223 @default.
- W3048155269 cites W4244689731 @default.
- W3048155269 doi "https://doi.org/10.1002/cbic.202000416" @default.
- W3048155269 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7756428" @default.
- W3048155269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32767537" @default.
- W3048155269 hasPublicationYear "2020" @default.
- W3048155269 type Work @default.
- W3048155269 sameAs 3048155269 @default.
- W3048155269 citedByCount "11" @default.
- W3048155269 countsByYear W30481552692021 @default.
- W3048155269 countsByYear W30481552692022 @default.
- W3048155269 countsByYear W30481552692023 @default.
- W3048155269 crossrefType "journal-article" @default.